A lentiviral vector with expression controlled by E2F-1: A potential tool for the study and treatment of proliferative diseases

Strauss, Bryan E.; Patrício, Juliana Rotelli; Carvalho, Anna Carolina Vieira de; Bajgelman, Marcio C.

doi:10.1016/j.bbrc.2006.08.007

Cited by 14 publications

(13 citation statements)

References 35 publications

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“…Compared to the saline-injected control cochlea, the injection of the vector into the inner ear does not increase the inflammatory reaction in the cochlea. Single lymphocytes were also detected in [2,19]. The HOX-GFP and WOX-GFP lentivirus vectors have been used previously by Pellinen et al and Salmon et al [2,20].…”

Section: Detection Of Cd4-and Cd8-b-positive Cells In the Inner Earmentioning

confidence: 99%

HOX-GFP and WOX-GFP lentivirus vectors for inner ear gene transfer

Pietola

Aarnisalo

Joensuu

et al. 2008

Acta Oto-Laryngologica

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HOX-GFP and WOX-GFP expression was restricted to the lining cells of the scala tympani and scala vestibuli. No GFP expression was seen in the organ of Corti or the spiral ganglion. Aminoglycoside treatment had no effect on the expression of these vectors. The distant spread of lentivirus vectors was minimal; only the liver of one animal showed some GFP expression. Inflammatory reaction caused by these vectors was mild. Few inflammatory cells were found in the perilymphatic space of the cochlea and in the vestibular organ.

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Section: Detection Of Cd4-and Cd8-b-positive Cells In the Inner Earmentioning

confidence: 99%

HOX-GFP and WOX-GFP lentivirus vectors for inner ear gene transfer

Pietola

Aarnisalo

Joensuu

et al. 2008

Acta Oto-Laryngologica

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“…Lentivirus was used as a vector to fluorescently‐label ADSCs. Fluorescence‐activated cell sorting and immunostaining of ADSCs transduced with lentivirus vector were performed . We observed that autologous ADSCs had been successfully cultured and labeled with fluorescence through confocal microscopy 72 hour post‐transduction.…”

Section: Methodsmentioning

confidence: 99%

Potential of autologous adipose‐derived stem cells to regenerate atrophied muscle in a rat model

Park

Kwon

2017

Wound Repair Regeneration

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Muscle atrophy results in severe functional impairment and is a significant clinical problem. We examined and characterized the therapeutic effects of autologous adipose-derived stem cells (ADSCs) using an in vivo muscle atrophy rat model. To identify the effect of injected ADSCs into muscle, we developed the following two models of muscle atrophy in rats: induction of denervation by sciatic nerve defects; and nerve repair after severing the sciatic nerve. The inguinal fat pads were harvested from each rat and autologous ADSCs were cultured and ADSCs were injected in the right hind limbs as the experimental group, while normal saline was injected in the left hind limbs, which served as the control group. After 2 weeks, gross examination and histologic analyses were performed. Additionally, to investigate the survival of ADSCs in muscle tissues, we traced the injected ADSCs. The fate of injected ADSCs into muscle was investigated using a green fluorescent protein (GFP) tagging method with lentivirus transfection. The muscle weight and cross-sectional area of muscle were greater and proliferation of connective tissue was less prominent in the ADSC-injected group. Alpha-bungarotoxin binding in the neuromuscular junction was significantly increased, and neoangiogenesis was higher in the ADSCs-injected group. green fluorescent protein-labeled ADSCs survived in the gastrocnemius muscle after 2 weeks. These findings could give a support in finding the role of autologous ADSCs as new therapeutic modality for regeneration of atrophied muscle.

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“…The retroviral plasmid pBABE-puro was used to perform S6K overexpression. Fragments of S6K isoforms were cloned into Bam HI/ Sal I restriction sites and retroviral-mediated gene transfer was performed as described previously [ 21 , 22 ].…”

Section: Methodsmentioning

confidence: 99%

S6Ks isoforms contribute to viability, migration, docetaxel resistance and tumor formation of prostate cancer cells

et al. 2016

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BackgroundThe S6 Kinase (S6K) proteins are some of the main downstream effectors of the mammalian Target Of Rapamycin (mTOR) and act as key regulators of protein synthesis and cell growth. S6K is overexpressed in a variety of human tumors and is correlated to poor prognosis in prostate cancer. Due to the current urgency to identify factors involved in prostate cancer progression, we aimed to reveal the cellular functions of three S6K isoforms–p70-S6K1, p85-S6K1 and p54-S6K2–in prostate cancer, as well as their potential as therapeutic targets.MethodsIn this study we performed S6K knockdown and overexpression and investigated its role in prostate cancer cell proliferation, colony formation, viability, migration and resistance to docetaxel treatment. In addition, we measured tumor growth in Nude mice injected with PC3 cells overexpressing S6K isoforms and tested the efficacy of a new available S6K1 inhibitor in vitro.ResultsS6Ks overexpression enhanced PC3-luc cell line viability, migration, resistance to docetaxel and tumor formation in Nude mice. Only S6K2 knockdown rendered prostate cancer cells more sensitive to docetaxel. S6K1 inhibitor PF-4708671 was particularly effective for reducing migration and proliferation of PC3 cell line.ConclusionsThese findings demonstrate that S6Ks play an important role in prostate cancer progression, enhancing cell viability, migration and chemotherapy resistance, and place both S6K1 and S6K2 as a potential targets in advanced prostate cancer. We also provide evidence that S6K1 inhibitor PF-4708671 may be considered as a potential drug for prostate cancer treatment.Electronic supplementary materialThe online version of this article (doi:10.1186/s12885-016-2629-y) contains supplementary material, which is available to authorized users.

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A lentiviral vector with expression controlled by E2F-1: A potential tool for the study and treatment of proliferative diseases

Cited by 14 publications

References 35 publications

HOX-GFP and WOX-GFP lentivirus vectors for inner ear gene transfer

HOX-GFP and WOX-GFP lentivirus vectors for inner ear gene transfer

Potential of autologous adipose‐derived stem cells to regenerate atrophied muscle in a rat model

S6Ks isoforms contribute to viability, migration, docetaxel resistance and tumor formation of prostate cancer cells

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