A light-regulated DNA-binding activity interacts with a conserved region of a Lemna gibba rbcS promoter.

Buzby, Jeffrey S.; Yamada, Takashi; Tobin, Elaine M.

doi:10.1105/tpc.2.8.805

Cited by 68 publications

(34 citation statements)

References 29 publications

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“…GT motifs, characterized in the pea rbcS-3A [ 14] and bean chs [17] genes were present in both positive and negative regulatory elements in the plant genes. The conserved sequence LRF-1, overlapping the TATA box, is associated with ligh-responsive expression in Lemna gibba [7] and tomato rbcS genes [27]. In addition, the Rca 5' sequences contain AT-rich regions which have been shown to enhance expression in rbcS and other genes [8,29,33].…”

Section: Introductionmentioning

confidence: 99%

Localization of light-inducible and tissue-specific regions of the spinach ribulose bisphosphate carboxylase/oxygenase (rubisco) activase promoter in transgenic tobacco plants

Orozco

Ogren

1993

Plant Mol Biol

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Deletions in the spinach rubisco activase (Rca) promoter in transgenic tobacco were analyzed to define the regions necessary for conferring light-inducible and tissue-specific expression. Transgenic plants were constructed with Bal 31 deletions of the Rca promoter fused to the coding region of the bacterial reporter gene beta-glucuronidase (GUS). Analysis of the Rca deletion mutants localized the region conferring normal expression downstream from -294 relative to the Rca transcription start site. A second set of transgenic plants containing the cauliflower mosaic virus (CaMV) 35S enhancer fused to the 3' end of the Rca/GUS constructs demonstrated the presence of a light-responsive element between -150 and -78 active in leaves. Regions 10 bp long within the light-responsive region, which included putative G box and GT elements, were removed by recombinant polymerase chain reaction. Deletion of the G box element resulted in a loss of gene expression in the leaves of transgenic tobacco, while deletion of the GT motif caused a 10-100-fold increase in expression in roots. However, site-directed mutagenesis of the GT motif resulted in expression patterns identical to the normal promoter. These experiments demonstrated that light-inducible and tissue-specific expression of the Rca promoter involves multiple cis elements proximal to the transcription start site, and that interactions between these elements are essential for regulating expression.

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Section: Introductionmentioning

confidence: 99%

Localization of light-inducible and tissue-specific regions of the spinach ribulose bisphosphate carboxylase/oxygenase (rubisco) activase promoter in transgenic tobacco plants

Orozco

Ogren

1993

Plant Mol Biol

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“…Of the reports of DNA-binding proteins associated with photoregulated genes, only one other factor (the Lemna gibba factor LRF-1) has been shown to exhibit a similar light-dependent binding activity to WF-1 in vitro [2]. However, it seems unlikely that WF-1 and LRF-1 are identical, as the LRF-1 binding site contains a conserved GATAAG motif which is not present in any of the fragments to which WF-1 binds.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years a number of transoacting proteins which interact with the upstream sequences of photoregulated genes have been identified [2,4,7,9,21]. In order to understand the mechanisms controlling expression of wheat chloroplast FBPase and SBPase, the genes encoding these enzymes have been isolated and sequenced [ 14,19].…”

Section: Introductionmentioning

confidence: 99%

A light- and developmentally-regulated DNA-binding interaction is common to the upstream sequences of the wheat Calvin cycle bisphosphatase genes

et al. 1993

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We have characterised a DNA-binding interaction common to the upstream sequences of the wheat fructose-1,6-bisphosphatase (FBPase) and sedoheptulose-1,7-bisphosphatase (SBPase) genes. The recognition site for this sequence-specific binding activity, designated wheat FBPase factor (WF-1), is located within 125 bp of the transcription start site of each gene. Within these regions there are no sequence motifs similar to those shown to be important for light-regulated expression in other species. The binding activity was not detected in wheat root nuclear extracts, or in pea leaf extracts. There was a higher level of binding activity in light-grown than in dark-grown wheat leaves. The level was also found to decline when light-grown plants were given an extended dark treatment, but could be reinduced by light. Utilising the gradient of developmental maturity which exists within the wheat leaf it was found that WF-1 activity increases during leaf development.

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“…However, other factors have been identified whose expression or binding activity has been found to be regulated by light [4,5,10,26,43,46,49], and these may co-operate with constitutive factors like GT-1 for full regulatory effect. Many of these factors bind to sequence elements containing a GATA motif.…”

Section: Introductionmentioning

confidence: 99%

The GATA-binding protein CGF-1 is closely related to GT-1

Teakle

Kay

1995

Plant Mol Biol

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Many light-regulated genes contain a conserved GATA motif in their 5'-upstream region. We have characterized in detail the GATA-binding factor, CGF-1, which bonds within a 73 bp TATA-proximal light/circadian regulatory element in the Arabidopsis cab2 promoter and to two more sites farther upstream. CGF-1 was found to be distinct from other metal-dependent GATA-binding factors, but to have the same sequence requirements for binding and similar physical and chemical properties as GT-1, a factor required for light regulation of the tobacco rbcS-3A gene. CGF-1 was found to be constitutively present in extracts and was shown to be immunologically related to GT-1. The close similarity between CGF-1 and GT-1 suggests that a GT-1-like factor is involved in the phytochrome/circadian regulation of the cab2 gene. CGF-1 and GT-1 were also found to have similar sequence specificities to another constitutively-regulated GATA factor, IBF-2b, which binds the I box region of the tomato nitrate reductase gene. Of three complexes detected using an IBF-2b-specific probe, only one was identical to CGF-1/GT-1. The other two were similar to IBF-2b, demonstrating that CGF-1/GT-1, although very similar, are actually distinct from IBF-2b. These data indicate that more than one factor can bind to the same short sequence and may indicate how constitutively present factors like GT-1 can play a role in light regulation.

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A light-regulated DNA-binding activity interacts with a conserved region of a Lemna gibba rbcS promoter.

Cited by 68 publications

References 29 publications

Localization of light-inducible and tissue-specific regions of the spinach ribulose bisphosphate carboxylase/oxygenase (rubisco) activase promoter in transgenic tobacco plants

Localization of light-inducible and tissue-specific regions of the spinach ribulose bisphosphate carboxylase/oxygenase (rubisco) activase promoter in transgenic tobacco plants

A light- and developmentally-regulated DNA-binding interaction is common to the upstream sequences of the wheat Calvin cycle bisphosphatase genes

The GATA-binding protein CGF-1 is closely related to GT-1

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