A lipidomic approach for profiling and distinguishing seed oils of Hibiscus manihot L., flaxseed, and oil sunflower

Abstract

As important oil crops in Inner Mongolia, sunflower, and flaxseed had distinct lipid profiles in seeds. As an emerging cash crop, Hibiscus manihot L. has strong potential market competitiveness. In this study, the lipidome, fatty acid composition and quality characteristics of flaxseed, H. manihot L., and sunflower seed oils were analyzed and compared. A total of 270 distinct lipids were identified and analyzed with an emerging detection approach-lipidomics, which illustrated the tremendous difference among the samples. triacylglycerol, diacylglycerol and polar lipids were the most abundant lipids in all samples. H. manihot L. seeds contained higher saturated and monounsaturated fatty acids and lower polyunsaturated fatty acids. H. manihot L. seed oil had the longest oxidative stability index time, high content of vitamin E and total phenolics, while flaxseed oil embodied the lowest oxidative stability. The peroxide value and acid value of the three oils were within the allowable range of Chinese national standards.

Study on the lipid and flavor compounds of Hibiscus manihot L. oil induced by different pressing method

“…As for the hot-pressing oil, the seeds were roasted in an oven at 160°C for 10 min, then extracted with the same way. Fatty acid (FA) composition and content were studied by derivatization to their methyl esters, which was consistent with the method described in Yang's article (Yang et al, 2022). The gas chromatography flame ionization detector (GC-FID; Agilent 7890A, Palo Alto, CA, USA) coupled with a capillary column (FFAP, 30 m, 0.25 mm i.d., 0.5 μm film thickness) was used.…”

“…The remaining supernatant was further extracted with 4 mL of dichloromethane, repeated twice, and the extracted liquids were combined. After nitrogen blowing, re-dissolve in 1 mL of isopropanol, filter through a 0.22 μm organic membrane to obtain the sample for testing (Yang et al, 2022). The sample extracts were analyzed using a UPLC-QTOF-MS system (HPLC, shimadzu UPLC LC-30A system; MS, AB Sciex TripleTOF ® 6600).…”

“…Vitamin E was detected by HPLC procedure according to the food safety national standard of China (GB5009.82-2016). The sample pre-treatment procedure was the same with Yang's (Yang et al, 2022). The samples were detected by HPLC of Shimadzu LC-20AT system equipped with a Thermo Scientific Acclaim C30 column (4.6×250mm, 5µm).…”

“…The content of Vitamin E were expressed in terms of α-tocopherol equivalents. The testing was carried out with three independent samples, with each sample detected in triplicate (Yang et al, 2022).…”

“…The total phenolic content was determined with the Folin-Ciocalteu's method. The sample pre-treatment procedure was the same with Yang's (Yang et al, 2022). Take 3 mL of sample liquid, add 1 mL of Folin phenol reagent, shake for 1 min and then add 3 mL of 10% Na 2 CO 3 solution, shake well and add water to 10 mL, and water bath in 45°C for 1.5 h. Use the solution without standard as a blank control, and determine the absorbance at 765 nm.…”