A liquid chromatographic method for simultaneous determination of amoxicillin sodium and sulbactam sodium in a combination formulation

Wang, Peng; Qi, Meiling; Sun, Yan; Yang, Jianjun

doi:10.1016/j.jpba.2004.07.015

Cited by 21 publications

(7 citation statements)

References 24 publications

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“…fluxacilline and metronidazole was realized by using HPLC [21][22][23][24][25][26], TLC 27 , CE 28 and chemometry 29 . In the present research, selective spectrophotometric estimation of amoxicillin based on the oxidative drug by Ce(IV) and coupling the product Ce(III) with arsenazo III (Scheme 2), which results to formation coloured products in acidic medium.…”

Section: Research Articlementioning

confidence: 99%

Indirect Spectrophotometric Determination of Amoxicillin by Oxidative with Cerium ammonium Nitrate Using Arsenazo III as a Reagent

2019

Chem Sci Trans

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A simple, accurate and sensitive spectrophotometric way used for preparation. The suggested method depends on using arsenazo III as reagent. The current method was depending on oxidative coupling reaction of amoxicillin by cerium ammonium nitrate with arsenazo III in the presence of sulfuric acid as acidic media. Then reaction Ce(III) with arsenazo III to form pink water-soluble dye product that has a maximum absorption at max 500 nm. Beers law is obeyed in the concentration of (1-10 µgmL-1). The molar absorptivity is 3x10 4 L.mol-1 .cm-1 , a sandal sensitivity of 0.012 µ gcm-2. Limit of detection (LOD) and limit of quantitation are 0.00874 µgmL-1 , (LOQ) 0.0291 µgmL-1 respectively. The analytical recovery of the new method was good (100.72%). The method successfully applied for the determination of Amoxicillin in pharmaceutical preparations.

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Section: Research Articlementioning

confidence: 99%

Indirect Spectrophotometric Determination of Amoxicillin by Oxidative with Cerium ammonium Nitrate Using Arsenazo III as a Reagent

2019

Chem Sci Trans

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“…A comprehensive review of the literature has revealed that there are several articles proposing methods for the simultaneous determination of amoxicillin and sulbactam [12][13][14][15][16]. Nevertheless, only one of them studied the quantification of sulbactam in the SP prodrug form [16].…”

Section: Introductionmentioning

confidence: 99%

A new reliable and rapid HPLC method for the simultaneous determination of amoxicillin and sulbactam pivoxil in pharmaceuticals. Application to both assay and dissolution samples

Razuc¹,

CABRERA²,

Garrido³

et al. 2022

jrp

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A fast, efficient and simple high performance liquid chromatographic method was developed and validated for the simultaneous determination of amoxicillin trihydrate (AMO) and sulbactam pivoxil (SP) in oral pharmaceutical dosage forms and, eventually, for their dissolution tests. The difference in molar absorptivities of these compounds constituted an analytical challenge, especially in formulations where AMO was in a higher proportion than SP. A reverse phase C18 column was used with a mobile phase consisting of acetonitrile and water (80:20 v/v) in isocratic mode. The retention times were found to be 2.26 min and 1.34 min for SP and AMO, respectively. The linearity range was evaluated over the concentration range of 2.5 and 250.0 µg mL -1 (correlation coefficients higher than 0.9998). The method was validated according to the International Conference on Harmonization ICH guidelines regarding selectivity, linearity, accuracy, precision, limit of detection, limit of quantification, system suitability and robustness. The validated method was applied for the quantification of AMO and SP in commercial tablets and oral suspensions (AMO/SP ratio between 1:1 and 7:1), being the first method in the open literature that enables the correct quantification of both active ingredients in formulations with an AMO/SP ratio higher than 1:1. Also, the new method was successfully applied for the dissolution study of AMO/SP formulations, which was reported for the first time in the open literature. According to the obtained results, the proposed method can be applied in the quality control of pharmaceuticals containing a combination of amoxicillin and sulbactam pivoxil.

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“…The determination of Amoxicillin in the presence of the sodium salt of Sulbactam, Clavulanic acid, Floxacillin and Metronidazole was realized by using LC [52][53][54] and chemometry [55].…”

Section: Introductionmentioning

confidence: 99%

Direct and indirect spectrophotometric determination of some selected antibiotics using potassium permanganate

El‐Didamony

Saad

El-Shaprawy

2013

Main Group Chemistry

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Two spectrophotometric methods were developed for the quantitative determination of Cefoperazone (CFP) and Amoxicillin (AMX) in pure forms as well as their active pharmaceutical ingredients (API). The methods are based on a redox reaction between the selected drugs and KMnO 4 in alkaline or acid media. Direct spectrophotometry (method A) involves treating the aqueous solution of the selected drugs with KMnO 4 in alkaline medium and measuring the bluish-green product at 610 nm. In indirect spectrophotometry (method B), the drug solutions were treated with a fixed concentration of KMnO 4 in acidic medium followed by the determination of the unreacted oxidant by measuring the decrease in absorbance of methylene blue at 665 nm. The different experimental parameters affecting the development and stability of the color were carefully studied and optimized. Beer's law is obeyed over 2.0-40 (method A) and 0.8-7.2 g/mL (method B). The molar absorptivity, Sandell sensitivity, detection and quantification limits were also calculated. The intra-and inter-day relative standard deviation (RSD) values demonstrated the validity of the two methods for the drugs in pure form and as the API. Statistical comparison of the results with a well-established official method showed excellent agreement and proved that there is no significant difference in the accuracy and precision.

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A liquid chromatographic method for simultaneous determination of amoxicillin sodium and sulbactam sodium in a combination formulation

Cited by 21 publications

References 24 publications

Indirect Spectrophotometric Determination of Amoxicillin by Oxidative with Cerium ammonium Nitrate Using Arsenazo III as a Reagent

Indirect Spectrophotometric Determination of Amoxicillin by Oxidative with Cerium ammonium Nitrate Using Arsenazo III as a Reagent

A new reliable and rapid HPLC method for the simultaneous determination of amoxicillin and sulbactam pivoxil in pharmaceuticals. Application to both assay and dissolution samples

Direct and indirect spectrophotometric determination of some selected antibiotics using potassium permanganate

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