A Liquid Chromatographic Method for the Simultaneous Determination of α-Tocopherol and Tocopherolquinone in Human Red Blood Cells and Other Biological Samples where Tocopherol Is Easily Oxidized during Sample Treatment

Vatassery, Govind T.; Smith, W. E.; Quach, Hung T.

doi:10.1006/abio.1993.1518

Cited by 63 publications

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“…The extraction procedure is essentially that of Vatassery et al (9) and includes a saponification of the matrix. This procedure, with addition of antioxidants, was found to avoid the oxidation of TH (12).…”

Section: Methods Developmentmentioning

confidence: 99%

“…Thus, biological samples must be handled with extreme care to preserve the true TQ/TH ratio, and the analytical procedure should assess the artifactual formation of TQ in each sample. The most commonly used method to quantify both TH and TQ in biological matrices makes use of reversephase HPLC with electrochemical detection (ECD), which allows both compounds to be detected at the 50 -100 pg level (7)(8)(9)(10). The main limitation of this technique is the lack of internal standards which can determine both the extraction efficiency of the compounds and the artifactual oxidation of TH during sample preparation (10).…”

mentioning

confidence: 99%

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Comparison of Gas Chromatography–Mass Spectrometry and Liquid Chromatography–Tandem Mass Spectrometry Methods to Quantify α-Tocopherol and α-Tocopherolquinone Levels in Human Plasma

Mottier

Gremaud

Guy

et al. 2002

Analytical Biochemistry

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Section: Methods Developmentmentioning

confidence: 99%

mentioning

confidence: 99%

Comparison of Gas Chromatography–Mass Spectrometry and Liquid Chromatography–Tandem Mass Spectrometry Methods to Quantify α-Tocopherol and α-Tocopherolquinone Levels in Human Plasma

Mottier

Gremaud

Guy

et al. 2002

Analytical Biochemistry

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“…Details of the method have been reported (Vatassery et al, 1997;Vatassery et al, 1993). Briefly, 2ml of ethanol containing 0.025% (w/v) butylated hydroxytoluene (BHT) and 0.1 ml of 30% (w/v) ascorbic acid were pipetted into tubes containing tissue homogenates.…”

Section: Biochemical Assaysmentioning

confidence: 99%

Mice lacking α-tocopherol transfer protein gene have severe α-tocopherol deficiency in multiple regions of the central nervous system

et al. 2008

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Ataxia with vitamin E deficiency is caused by mutations in α-tocopherol transfer protein (α-TTP) gene and it can be experimentally generated in mice by α-TTP gene inactivation (α-TTP-KO). This study compared α-tocopherol (α-T) concentrations of five brain regions and of four peripheral organs from 5 months old, male and female, wild-type (WT) and α-TTP-KO mice. All brain regions of female WT mice contained significantly higher α-T than those from WT males. α-T concentration in the cerebellum was significantly lower than that in other brain regions of WT mice. These sex and regional differences in brain α-T concentrations do not appear to be determined by α-TTP expression which was undetectable in all brain regions. All the brain regions of α-TTP-KO mice were severely depleted in α-T. The concentration of another endogenous antioxidant, total glutathione, was unaffected by gender but was decreased slightly but significantly in most brain regions of α-TTP-KO mice. The results show that both gender and the hepatic α-TTP, but not brain α-TTP gene expression are important in determining α-T concentrations within the brain. Interestingly, functional abnormality (ataxia) develops only very late in α-TTP-KO mice in spite of the severe α-tocopherol deficiency in brain starting at an early age.

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“…Erythrocytes previously washed three times with isotonic phosphate-buffered saline, pH 7.4, and stored at –72°C in the presence of pyrogallol, ascorbate and butylated hydroxytoluene, were used for the assay [18]. The HPLC instrumental parameters were as follows: Spherisorb ODS 2 (5 µm packing), 40 × 250 mm, Pharmacia LKB column, mobile phase: methanol/water/2-propanol (91.25:4.25:4.5) containing 2.8 g/l sodium perchlorate, flow rate: 1.5 ml/min, electrochemical detection (680 mV, Electrochemical Detector, Pharmacia).…”

Section: Methodsmentioning

confidence: 99%

Alpha-Tocopherol and NADPH in the Erythrocytes and Plasma of Multiple Sclerosis Patients

et al. 2003

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Objectives: To investigate the influence of interferon-β-1b (INF-β-1b) therapy on blood antioxidants (α-tocopherol and NADPH) in multiple sclerosis (MS). Patients and Methods: Patients with relapsing-remitting MS (n = 14) have been studied during 6 months of INF-β-1b therapy. α-Tocopherol was determined by HPLC and UV or electrochemical detection; NADPH was quantified spectrophotometrically. Results: The erythrocyte α-tocopherol level was reduced (p < 0.001) before treatment, but had regained the control level by 6 months of therapy. The plasma α-tocopherol/lipid ratios were constant during therapy. Plasma triglyceride levels were transiently increased (p = 0.0270) after 1 month of treatment. INF-β-1b had also induced a transient decrease in NADPH after 1 month, but thereafter the level returned to approximately the initial value (p = 0.0248). Conclusion: INF-β-1b seems to exert a sparing effect toward the erythrocyte α-tocopherol content. The fall in NADPH in parallel to the rise in plasma triglycerides suggests stimulation of fatty acid synthesis by INF-β-1b.

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A Liquid Chromatographic Method for the Simultaneous Determination of α-Tocopherol and Tocopherolquinone in Human Red Blood Cells and Other Biological Samples where Tocopherol Is Easily Oxidized during Sample Treatment

Cited by 63 publications

References 0 publications

Comparison of Gas Chromatography–Mass Spectrometry and Liquid Chromatography–Tandem Mass Spectrometry Methods to Quantify α-Tocopherol and α-Tocopherolquinone Levels in Human Plasma

Comparison of Gas Chromatography–Mass Spectrometry and Liquid Chromatography–Tandem Mass Spectrometry Methods to Quantify α-Tocopherol and α-Tocopherolquinone Levels in Human Plasma

Mice lacking α-tocopherol transfer protein gene have severe α-tocopherol deficiency in multiple regions of the central nervous system

Alpha-Tocopherol and NADPH in the Erythrocytes and Plasma of Multiple Sclerosis Patients

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