A liquid chromatography/electrospray ionization mass spectrometry (LC–MS/MS) assay for the determination of irinotecan (CPT-11) and its two major metabolites in human liver microsomal incubations and human plasma samples

Simultaneous Determination of Irinotecan and Its Metabolite 7-Ethyl-10-hydroxycamptothecin in Biological Fluids Using Excitation-emission Matrix Fluorescence Coupled with Second-order Calibration Method

“…ANWE 算法是本实验室提出来的一种性能优良的 算法, 诸多的研究 [19,23] 表明 ANWE 算法对复杂体系中 感兴趣组分测定的应用很成功, 而且性能要略优于 Figure 1 Core consistency values as a function of the trial number of component for the analysis of the spiked plasma and urine 中, HPLC 方法测定羊血浆中的 CPT11 和 SN38, 得 到的 LOQ 都是 5 ng/mL, 文献 [37] 中采用 HPLC-MS 联用 技术, 得到的人血浆中 CPT11 和 SN38 的 LOQ 分别是 …”

A two-step pH-dependent liquid–liquid extraction combined with HPLC-fluorescence method for the determination of 10-hydroxycamptothecin in mouse liver tissue

“…The pharmacokinetics of CPTs in plasma samples by HPLC with fluorescence detection has been well established (Guo et al, 2011a;Liu et al, 2008;Loh & Ahmed, 1990;Sun et al, 2004). The biological samples were prepared using relatively simple approaches such as protein precipitate with acetonitrile (ACN)/methanol (Fraier et al, 2000;Guo et al, 2003), SPE (D'Esposito et al, 2008;Fraier et al, 2000) or extraction with organic solvents using a conventional LLE (Koshkina et al, 1999;Liu et al, 2008;Sun et al, 2004;Zhao et al, 2006). However, the same sample preparation methods for plasma samples were used for tissue samples in most cases (Koshkina et al, 1999;Wu & Zhang, 2001).…”

In Situ Intestinal Perfusion of Irinotecan: Application to P-gp Mediated Drug Interaction and Introduction of an Improved HPLC Assay

“…Many of them required complicated and expensive equipments such as LC-MS or fluorescence detectors with the capability of switching wavelength (15)(16)(17)). UV detection is considered to be simple and most accessible for the research community.…”