A localized calcium transient and polar body abscission

Mo, Guolong; Swider, Zachary T.; Leblanc, Julie; Li, Ruizhen

doi:10.1080/15384101.2022.2092815

Cited by 2 publications

(1 citation statement)

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“…In addition to its essential function in maintaining the structural integrity of cell walls and membrane systems, it has been found to operate as an intracellular regulator in several areas of plant growth and development, including cell division and elongation and fruit formation. The Ca 2+ deficit produced by the downregulation of genes producing Ca 2+ ion transporters may be an indicator of apple lateral fruit decay, and the Ca 2+ signaling system is essential for yellow lupin pod abscission, according to previous studies (Glazińska et al, 2019;Mo et al, 2022). In addition, genes involved in the Ca 2+ signaling pathway Screening of transcription factor family regulators differentially expressed in CF and CHF.…”

Section: Discussionmentioning

confidence: 89%

Transcriptome and targeted hormone metabolome reveal the molecular mechanisms of flower abscission in camellia

et al. 2022

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IntroductionCamellia is among the most ornamentally valuable flowers and plants worldwide. Flower abscission typically causes significant financial losses by the horticultural landscape. Previous research has revealed that phytohormones, transcription factors, and other genes involved in floral development regulate the maintenance and mortality of flowersMethodsIn this study, for the first time, the transcriptomes and targeted hormone metabolomics of three developmental stages of the receptacles of two distinct camellia strains (CF: abscission strain, CHF: nonabscission strain) were analyzed to determine their roles in regulating blossom abscission in camellia.ResultsABA content was shown to be considerably upregulated throughout all phases of CF development, as were the genes implicated in the ABA production pathway and their downstream counterparts. Highly expressed genes in CF were involved in galactose metabolism, phenylpropanoid biosynthesis, amino and nucleotide sugar metabolism, pentose and glucuronate interconversions, and MAPK. Among others, highly expressed genes in CHF are associated with fructose and mannose metabolism, alpha-linolenic acid metabolism, biosynthesis of secondary metabolites, starch and sucrose metabolism, and cutin, suberin, and wax biosynthesis. A vast variety of stress response-related pathways and redox-related activities were also shown to be active in CHF. In contrast, CF dramatically activated pathways associated with lignin production, keratinogenesis, cell wall biogenesis, and ABA response. A comparative transcriptomic study of the CF and CHF pathways revealed that the downstream response pathways of hormones, including CTK, BR, IAA, ethylene, and GA, were very active in CF, indicating a significant amount of signal transduction and transcriptional regulation by CF. In addition, members of the transcription factor family, such as MYB, bHLH, MADS, and WD40, may regulate flower abscission.DiscussionA comparative transcriptome analysis of two distinct strains of camellia receptacles elucidates the molecular processes and regulatory characteristics of flower abscission and provides direction for the targeted improvement and breeding of camellia.

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Section: Discussionmentioning

confidence: 89%

Transcriptome and targeted hormone metabolome reveal the molecular mechanisms of flower abscission in camellia

et al. 2022

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Estrogen Regulates Ca2+ to Promote Mitochondrial Function Through G-Protein-Coupled Estrogen Receptors During Oocyte Maturation

Liu,

Li,

et al. 2024

Biomolecules

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Estrogen is a steroid hormone that plays a key role in regulating many physiological processes, such as follicle activation and development and oocyte maturation in mammals. Ca2+ is crucial in oogenesis, oocyte maturation, ovulation, and fertilization. However, the mechanism by which estrogen regulates Ca2+ during oocyte maturation in mice has not been reported. This study revealed that Ca2+ levels in oocytes significantly increase during the 4–12 h period in vitro. Oocytes treated with 0.1 µM estrogen and 1 µM G1, a G-protein-coupled estrogen receptor (GPER) agonist, showed significantly increased Ca2+ levels, while treatment with 1 µM G15, an antagonist of GPER, significantly decreased Ca2+ levels. Notably, estrogen regulates Ca2+ in oocytes through the GPER pathway and promotes the expression of the Ca2+-producing protein EPAC1. In addition, estrogen alleviates the inhibitory effect of the Ca2+ chelator BAPTA-AM during oocyte maturation by promoting Ca2+ production. Furthermore, estrogen can promote the expression of the mitochondrial generation-associated protein SIRT1 through the GPER pathway, alleviate mitochondrial oxidative damage caused by BAPTA-AM, and restore the mitochondrial membrane potential level. Collectively, this study demonstrates that estrogen can regulate Ca2+ through the GPER-EPAC1 pathway and promote the expression of SIRT1, which promotes oocyte mitochondrial function during oocyte maturation.

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A localized calcium transient and polar body abscission

Cited by 2 publications

References 54 publications

Transcriptome and targeted hormone metabolome reveal the molecular mechanisms of flower abscission in camellia

Transcriptome and targeted hormone metabolome reveal the molecular mechanisms of flower abscission in camellia

Estrogen Regulates Ca2+ to Promote Mitochondrial Function Through G-Protein-Coupled Estrogen Receptors During Oocyte Maturation

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