A low-cost, accurate method for detecting reticulocytes at different maturation stages based on changes in the mitochondrial membrane potential

Du, Ruilan; Bei, Haikang; Jia, Lihong; Huang, Chunyan; Chen, Qizhu; Wang, Jinquan; Wu, Fenglin; Chen, Jun; Bo, Huaben

doi:10.1016/j.vascn.2019.106664

Cited by 4 publications

(3 citation statements)

References 26 publications

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“…Numerous mechanisms of reticulocyte maturation have been described but it has been unclear as to whether these occur simultaneously or in stages. Our data, and that of others ( Mel et al, 1977 ; Koury et al, 2005 ; Mankelow et al, 2016 ; Rhodes et al, 2016 ; Du et al, 2020 ; Mei et al, 2021 ), suggests that different mechanisms of maturation occur at different stages. Mitochondria and lysosomes predominantly being lost early probably through an autophagy and exosome release mechanism.…”

Section: Discussionsupporting

confidence: 80%

“…Studies have shown that during maturation the level of RNA, as measured by thiazole orange (TO), and TfR in the reticulocyte reduces and these levels are often used to define the different stages of maturation from R1 (nascent reticulocyte, high CD71, high RNA) to R2 (intermediate reticulocyte, low CD71, low RNA) to R3 (mature RBC, CD71 negative, RNA negative) ( Malleret et al, 2013 ). Recently, another method for classifying different stages of reticulocyte maturation has been described that uses changes in mitochondrial membrane potential ( Du et al, 2020 ). Despite all these classifications, exactly how the nascent reticulocyte matures into a RBC is not fully understood.…”

Section: Introductionmentioning

confidence: 99%

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Reticulocyte Maturation and Variant Red Blood Cells

et al. 2022

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The bone marrow produces billions of reticulocytes daily. These reticulocytes mature into red blood cells by reducing their plasma membrane by 20% and ejecting or degrading residual internal organelles, membranes and proteins not required by the mature cell. This process occurs by autophagy, protein degradation and vesiculation but is not well understood. We previously reported that Southeast Asian Ovalocytic RBCs demonstrate incomplete reticulocyte maturation and we have now extended this study to a number of other variant RBCs. By comparing the profile of a pure reticulocyte preparation of cultured red cells with these variant cells, we show that the largest of these cells, the overhydrated hereditary stomatocytosis cells, are the least mature, they barely reduced their plasma membrane and contain large amounts of proteins that should have been reduced or removed. Intermediate sized variant RBCs appear to be more mature but retain some endoplasmic reticulum and residual membrane proteins. We propose that the size and composition of these variant cell types correlate with the different stages of reticulocyte maturation and provide insight into the reticulocyte maturation process.

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Reticulocyte Maturation and Variant Red Blood Cells

et al. 2022

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“…Mitochondrial membrane potential (MMP) refers to the negative potential difference between the two sides of the inner mitochondrial membrane. It is a sensitive indicator for evaluating mitochondrial function (90)(91)(92)(93). It is closely associated with cellular homeostasis and is most commonly used to determine the metabolic state of mitochondria (93)(94)(95)(96)(97)(98).…”

Section: Determination Of Mitochondrial Membrane Potentialmentioning

confidence: 99%

Common methods in mitochondrial research (Review)

Yin

Shen

2022

Int J Mol Med

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Mitochondrial abnormalities are primarily seen in morphology, structure and function. They can cause damage to organs, including the heart, brain and muscle, by various mechanisms, such as oxidative stress, abnormal energy metabolism, or genetic mutations. Identifying and detecting pathophysiological alterations in mitochondria is the principal means of studying mitochondrial abnormalities. The present study reviewed methods in mitochondrial research and focused on three aspects: Mitochondrial extraction and purification, morphology and structure and function. In addition to classical methods, such as electron microscopy and mitochondrial membrane potential monitoring, newly developed methods, such as mitochondrial ultrastructural determination, mtdNA mutation assays, metabolomics and analyses of regulatory mechanisms, have also been utilized in recent years. These approaches enable the accurate detection of mitochondrial abnormalities and provide guidance for the diagnosis and treatment of related diseases. Contents1. Introduction 2. Extraction and purification of mitochondria 3. determination of mitochondrial morphology and structure 4. determination of mitochondrial function 5.

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Gut microbiota affects the efficacy of Danggui Buxue Tang by affecting plasma concentration of active ingredients

Chen

Huang

et al. 2021

Journal of Ethnopharmacology

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A low-cost, accurate method for detecting reticulocytes at different maturation stages based on changes in the mitochondrial membrane potential

Cited by 4 publications

References 26 publications

Reticulocyte Maturation and Variant Red Blood Cells

Reticulocyte Maturation and Variant Red Blood Cells

Common methods in mitochondrial research (Review)

Gut microbiota affects the efficacy of Danggui Buxue Tang by affecting plasma concentration of active ingredients

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