A low-cost and portable fluorometer based on an optical pick-up unit for chlorophyll-a detection

Chen, Xu; Du, Jing; Kanwal, Saima; Yang, Zhi-Jin; Zheng, Lu-Lu; Wang, Jian; Wen, Jing; Zhang, Da-Wei

doi:10.1016/j.talanta.2023.125447

Cited by 4 publications

(1 citation statement)

References 52 publications

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“…Moreover, the OPU features a built-in laser-diode driver, necessitating solely the input of different level voltages to specific pins. The relevant details are available in our previous work [ 32 ]. The laser power emitted by the laser diode in the OPU can be adjusted by the pulse width modulation (PWM) signal input from the external control board.…”

Section: Methodsmentioning

confidence: 99%

A Portable Fluorescent Lateral Flow Immunoassay Platform for Rapid Detection of FluA

Chen,

Huang,

Kanwal

et al. 2024

Biosensors

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The spread of the FluA virus poses significant public health concerns worldwide. Fluorescent lateral flow immunoassay (LFIA) test strips have emerged as vital tools for the early detection and monitoring of influenza infections. However, existing quantitative virus-detection methods, particularly those utilizing smartphone-based sensing platforms, encounter accessibility challenges in resource-limited areas and among the elderly population. Despite their advantages in speed and portability, these platforms often lack user-friendliness for these demographics, impeding their widespread utilization. To address these challenges, this study proposes leveraging the optical pick-up unit (OPU) sourced from commercial optical drives as a readily available fluorescence excitation module for the quantitative detection of antibodies labeled with quantum-dot fluorescent microspheres. Additionally, we utilize miniaturized and high-performance optical components and 3D-printed parts, along with a customized control system, to develop an affordable point-of-care testing (POCT) device. Within the system, a stepping motor scans the test strip from the T-line to the C-line, enabling the calculation of the fluorescence-intensity ratio between the two lines. This simple yet effective design facilitates rapid and straightforward field or at-home testing for FluA. The proposed prototype platform demonstrates promising performance, achieving a limit of detection (LOD) of 2.91 ng/mL, a total detection time of no more than 15 min, and dimensions of 151 mm × 11.2 mm × 10.8 mm3. We believe that the proposed approach holds great potential for improving access to an accurate influenza diagnosis.

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Section: Methodsmentioning

confidence: 99%

A Portable Fluorescent Lateral Flow Immunoassay Platform for Rapid Detection of FluA

Chen,

Huang,

Kanwal

et al. 2024

Biosensors

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Tailoring molecularly imprinted polymer on titanium-multiwalled carbon nanotube functionalized gold electrode for enhanced chlorophyll determination in microalgae health assessment

Ramanathan,

Lau,

Goh

et al. 2024

Microchim Acta

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Point sources for digital lensless holographic microscopy: comparative assessment

Buitrago-Duque,

Patiño-Jurado,

Garcia-Sucerquia

2024

Opt. Eng.

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The light source in digital lensless holographic microscopy (DLHM) plays a key role in the optical performance of this microscopy methodology. The underlying physics that support DLHM rely on having an outward propagating spherical wavefront illuminating the sample to record on the surface of a digital camera the intensity of the diffracted wavefield that is magnified by free-space propagation; this first stage constitutes the recording. The information of the sample is numerically retrieved by computing the diffraction that an inward propagating spherical wavefront undergoes in the recorded intensity in a stage known as reconstruction. In both stages of this lensless microscopy method, it is assumed that perfect spherical wavefronts are utilized; hence, the closer the illumination light sources to exp½AEikr ∕jr j are, the better the performance of the microscope. For the recovery of the information of the sample, the accuracy of the inward propagating spherical wavefront exp½−ikr ∕jr j is guaranteed by its correct sampling in the numerical computation of the diffraction process; thus, the main challenge is the physical production of light sources to illuminate the sample whose amplitude profile is accurately described by exp½ikr ∕jr j. In this work, the different approaches that our research group has explored to produce illuminating point sources for DLHM are evaluated. Point sources based on pinholes, coned-shaped optical fibers, optical pick-up units, and aspheric lenses are evaluated by rating their robustness, compactness, reliability, cost and easiness of production, and their performance on imaging test targets and biosamples. Some additional DLHM light sources reported in the literature are also briefly reviewed based on their available data.

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A low-cost and portable fluorometer based on an optical pick-up unit for chlorophyll-a detection

Cited by 4 publications

References 52 publications

A Portable Fluorescent Lateral Flow Immunoassay Platform for Rapid Detection of FluA

A Portable Fluorescent Lateral Flow Immunoassay Platform for Rapid Detection of FluA

Tailoring molecularly imprinted polymer on titanium-multiwalled carbon nanotube functionalized gold electrode for enhanced chlorophyll determination in microalgae health assessment

Point sources for digital lensless holographic microscopy: comparative assessment

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