A low-density lipoprotein receptor (LDLR) class A domain-containing C-type lectin from Litopenaeus vannamei plays opposite roles in antibacterial and antiviral responses

Liang, Zhiwei; Yang, Linwei; Zheng, Jiefu; Zuo, Hongliang; Weng, Shaoping; He, Jianguo; Xu, Xiaopeng

doi:10.1016/j.dci.2018.11.002

Cited by 39 publications

(10 citation statements)

References 41 publications

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“…However, the underlying regulatory mechanism of CTLs is complicated and we are still far from understanding it. As shown in a previous study, it was found that the silencing of LvLdlrCTL (a CTL containing a low-density lipoprotein receptor (LDLR) class A domain) from L. vannamei down-regulated the expression of 3 AMPs (crustin, ALF3, and PEN3), but 2 other AMPs (PEN2 and PEN4) were upregulated (70). Similarly, in our study, 2 AMPs (SpCrustin3 and SpALF6) were upregulated at 3 h in the hemocytes, while another AMP SpALF2 was down-regulated at 24 h in the hemocytes after rSpCTL6 treatment.…”

Section: Discussionmentioning

confidence: 64%

A New C-Type Lectin Homolog SpCTL6 Exerting Immunoprotective Effect and Regulatory Role in Mud Crab Scylla paramamosain

Qiu

Chen

et al. 2021

Front. Immunol.

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C-type lectin (CTL), a well-known immune-related molecule, has received more and more attention due to its diverse functions, especially its important role in development and host defense of vertebrate and invertebrate. Since the research on crab CTLs is still lack, we screened a new CTL homolog, named SpCTL6 from mud crab Scylla paramamosain. The full-length cDNA sequence of SpCTL6 was 738 bp with a 486 bp of ORF, and the deduced amino acids were 161 aa. SpCTL6 was predicted to have a 17 aa signal peptide and its mature peptide was 144 aa (MW 16.7 kDa) with pI value of 5.22. It had typical CTL structural characteristics, such as a single C-type lectin-like domain, 4 conserved cysteines, similar tertiary structure to that of vertebrate CTLs and a mutated Ca2+ binding motif Gln-Pro-Thr (QPT), clustering into the same branch as the crustacean CTLs. SpCTL6 was highly expressed in the entire zoeal larval stages and widely distributed in adult crab tissues with the highest transcription level in testis. During the molting process of juvenile crabs, the expression level of SpCTL6 was remarkably increased after molting. SpCTL6 could be significantly upregulated in two larval stages (Z1 and megalopa) and adult crab testis under immune challenges. Recombinant SpCTL6 (rSpCTL6) was successfully obtained from eukaryotic expression system. rSpCTL6 exhibited binding activity with PAMPs (LPS, lipoteichoic acid, peptidoglycan, and glucan) and had a broad spectrum bacterial agglutination activity in a Ca2+-dependent manner. In addition, rSpCTL6 could enhance the encapsulation activity of hemocytes and has no cytotoxic effect on hemocytes. Although rSpCTL6 had no bactericidal activity on Vibrio alginolyticus, rSpCTL6 treatment could significantly reduce the bacterial endotoxin level in vitro and greatly improved the survival of S. paramamosain under V. alginolyticus infection in vivo. The immunoprotective effect of rSpCTL6 might be due to the regulatory role of rSpCTL6 in immune-related genes and immunological parameters. Our study provides new information for understanding the immune defense of mud crabs and would facilitate the development of effective strategies for mud crab aquaculture disease control.

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Section: Discussionmentioning

confidence: 64%

A New C-Type Lectin Homolog SpCTL6 Exerting Immunoprotective Effect and Regulatory Role in Mud Crab Scylla paramamosain

Qiu

Chen

et al. 2021

Front. Immunol.

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“…PRRs can recognize pathogen‐associated molecular patterns present on the surface of microorganism but absent in the host; thus, it triggers a cascade of immune responses such as agglutination, encapsulation, nodulation, phagocytosis, the release of antimicrobial peptides and activation of the ProPO system leading to melanization and promotes the degradation and clearance of pathogenic microorganism (Liang et al, 2019; Tran et al, 2020; Wang et al, 2009). To date, 11 types of PRRs have been identified in shrimp, including β‐1,3‐glucanase‐related proteins, β‐1,3‐glucan‐binding proteins, CTLs, scavenger receptors, galectins, fibrinogen‐related proteins, thioester‐containing protein, down syndrome cell adhesion molecule, serine protease homologs, trans‐activation response RNA‐binding protein and Toll‐like receptors (Wang & Wang, 2013b).…”

Section: Discussionmentioning

confidence: 99%

Screening of candidate immune‐related unigenes from exoskeleton of Macrobrachium nipponense through transcriptome analysis

Yue

Peng

et al. 2022

Aquaculture Research

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The exoskeleton of a few crustacean species possesses additional antimicrobial activities to defend invading pathogens than merely a simple physical barrier. However, the genetic links underlying are apparently still lacking. Aiming at establishing the link between the genetic cause and the antimicrobial function, the transcriptomes of exoskeleton of Macrobrachium nipponense, coupled with or without the challenge of Aeromonas hydrophila, were sequenced and analysed. Using the Illumina RNA‐Seq method, 41,261 unigenes were obtained in total, 6540 of which were differentially expressed. All differentially expressed unigenes (DEGs) were analysed by GO and KEGG. Interestingly, a total of 131 immune‐related genes were spotted and grouped into 12 varied functions and 3 different KEGG pathways, among which the pattern recognition receptors (PRRs) make up the largest population. Furthermore, five c‐type lectins (CTLs) were selected from DEGs for further analysis and it was found that all CTLs are closely correlated with moult cycle and constitutively expressed in tested samples except haemocytes. Upon the challenge of A.hydrophila, their transcript levels (CTLs) were found highly dynamic, reduced in the beginning but increased later on. Together, our results suggest that cuticular CTLs is most likely involved in the defence of A.hydrophila, which establish the foundation for investigating immune response of crustacean exoskeleton and constructing possible strategies to prevent the spread of A. hydrophila in aquaculture of crustaceans.

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“…2014a; Liang et al . 2019) activities. The only L‐type lectin, LvLTLC1, currently reported in P. vannamei , exhibits only antibacterial activity (Tian et al .…”

Section: Antimicrobial and Antimicrobial‐like Moleculesmentioning

confidence: 99%

The expanding repertoire of immune‐related molecules with antimicrobial activity in penaeid shrimps: a review

Aweya

Zheng

et al. 2021

Reviews in Aquaculture

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Shrimps live in a complex aquatic ecosystem with numerous other biotic and abiotic factors, especially pathogenic microorganisms, which require the adaptation of prudent protective and survival strategies. As invertebrates, shrimps reply on humoral and cellular innate immune response mechanisms for protection against pathogens. One integral part of the humoral innate immune defence mechanism involves many biomolecules that are constitutively expressed or induced by pathogens. Shrimps, therefore, produce various biomolecules and secondary metabolites for physiological and pathophysiological purposes, most of which also act as effector molecules in immune-related functions. A growing number of molecules have been identified as essential constituents of the immune arsenal of biomolecules in penaeid shrimps. This review provides a synthesis of important information on penaeid shrimp-encoded immune-related molecules with antimicrobial activities, their molecular regulation and importance in shrimp innate immune response. Research gaps would be identified and proposed research themes emphasized for future studies.

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A low-density lipoprotein receptor (LDLR) class A domain-containing C-type lectin from Litopenaeus vannamei plays opposite roles in antibacterial and antiviral responses

Cited by 39 publications

References 41 publications

A New C-Type Lectin Homolog SpCTL6 Exerting Immunoprotective Effect and Regulatory Role in Mud Crab Scylla paramamosain

A New C-Type Lectin Homolog SpCTL6 Exerting Immunoprotective Effect and Regulatory Role in Mud Crab Scylla paramamosain

Screening of candidate immune‐related unigenes from exoskeleton of Macrobrachium nipponense through transcriptome analysis

The expanding repertoire of immune‐related molecules with antimicrobial activity in penaeid shrimps: a review

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