A low-toxic artificial fluorescent glycoprotein can serve as an efficient cytoplasmic labeling in living cell

Si, Jiangju; Liang, Dawei; Kong, Dan; Wu, Sufang; Yuan, Lamei; Xiang, Yan; Jiang, Lei

doi:10.1016/j.carbpol.2014.08.118

Cited by 1 publication

(1 citation statement)

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“…Functional micelle, a new kind of self-assembly supramolecular system, which possesses hydrophilic and hydrophobic chemical functional groups and could simulate the active center and the hydrophobic microenvironment of enzyme, attracts many attentions of chemists and biologists [28][29][30]. In this work, a novel functional surfactant with Lhistidine (His) residue was synthesized and its micelle was used as mimic of endo-b-1,4-glucanase to catalyzed the hydrolysis of methyl-b-D-cellobioside, which is regarded as a good structural unit model of cellulose, in weakly acidic aqueous solution at relatively low temperatures (80-110°C).…”

Section: Introductionmentioning

confidence: 99%

Effective Cleavage of β-1,4-Glycosidic Bond by Functional Micelle with l-Histidine Residue

Liao¹,

Liu

Xiao³

et al. 2016

Catal Lett

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A novel functional surfactant N a -dodecyl-Lhistidine (NDH) was synthesized and its micelle was used as mimic of b-1,4-endoglucanase to catalyzed the hydrolysis of methyl-b-D-cellobioside (MCB) under relatively low temperatures (80-110°C). The results showed that the micelle of NDH displayed excellent catalytic activity for the cleavage of b-1,4-glycosidic bond and the formation ofThe micelle-catalyzed first-order reaction rate constant k m of degradation of MCB was calculated. The conversion of MCB and selectivity of reducing sugars (glucose and fructose) could reach 58.9 and 87.1 %, respectively, for a reaction time of 10 h at pH 4.0 and 110°C. In NDH micelle-catalyzed reaction sugar ester was deduced as the chief product. The reaction pathways of MCB hydrolysis were proposed and the activation energy Ea for the hydrolysis was calculated. Graphical AbstractO OCR OH OH HO HO pH4.0, 90 o C Selectivity>97% O O OH OH HO HO O OH OH OCH 3 HO Glucose Fructose + p H 1 2 . 0 , 2 5 o C O N N H NH HOOC C 12 H 25

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Section: Introductionmentioning

confidence: 99%