A Lysosome-Targetable and Two-Photon Fluorescent Probe for Monitoring Endogenous and Exogenous Nitric Oxide in Living Cells

Abstract

A lysosome-specific and two-photon fluorescent probe, Lyso-NINO, demonstrates high selectivity and sensitivity toward NO, lower cytotoxicity, and perfect lysosomal localization. With the aid of Lyso-NINO, the first capture of NO within lysosomes of macrophage cells has been achieved using both two-photon fluorescence microscopy and flow cytometry.

Plasmonic Nanoprobes for Real‐Time Optical Monitoring of Nitric Oxide inside Living Cells

“…[8] Next, the same protocol was followed with unlabeled cells and capsules ( Figure S6). Contrary to other sensors, [13] our sensor turned out to be highly viable and compatible with the cells. Even in the presence of capsules, 97 % of cells were viable (see the Supporting Information).…”

“…[11] A variety of NO-sensitive fluorescent molecular probes have been developed to image intracellular NO under one- [12] or two-photon microspectroscopy. [13] These methods, some of which are commercial (e.g. OxiSelect, Cell Biolabs; DAR-4M AM, Calbiochem; DAF-FM DA, Sigma Aldrich), have enhanced the understanding about NO homeostasis, but the probes are in general toxic, can degrade inside the lysosomes, or suffer from photobleaching upon repetitive illumination with light when monitoring for a long time.…”

A Lysosome‐Targeting AIEgen for Autophagy Visualization

“…[ 31 ] Once entered lysosome, the morpholine will be protonated due to the high acidity of the lysosome, which endows AIE-LysoY with higher hydrophilicity and lysosomal retention. [ 32 ] In this way, fl uorescence of AIE-LysoY can only be observed in the lysosome area.…”

A reversible fluorescence chemosensor for sequentially quantitative monitoring copper and sulfide in living cells

“…The cytotoxicity of FL toward the breast carcinoma cells, MDA-MB-231 was investigated by the reduction activity of the methyl thiazolyl tetrazolium (MTT, [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]) assay [58][59][60]. As shown in Fig.…”