A lysosome-targeted fluorescent probe based on a BODIPY structure for Cys/Hcy detection

Zhang, Wenxuan; Wu, Binbin; Liang, Manshan; Zhang, Mengpei; Hu, Yutao; Huang, Zu-Sheng; Ye, Xiaoxia; Du, Bing; Quan, Yun-Yun; Jiang, Yongsheng

doi:10.1039/d3ay01965a

“…The existing detection methods of homocysteine include high performance liquid chromatography (HPLC), 8 fluorescence, 9 electrochemical detection, 10,11 etc . Among these methods, HPLC is regarded as the most recognized and reproducible method, but it requires complex pre-processing steps before detection, expensive instruments and skilled personnel, and fails to meet the requirements of rapid detection.…”

Section: Introductionmentioning

confidence: 99%

A lateral flow strip for on-site detection of homocysteine based on a truncated aptamer

Wu,

Huang,

Chen

et al. 2024

Anal. Methods

0

View full text Add to dashboard Cite

show abstract

A FRET Autophagy Imaging Platform by Macrocyclic Amphiphile

Jiang,

Chen,

Chen

et al. 2024

Angewandte Chemie

0

View full text Add to dashboard Cite

Autophagy is a ubiquitous process of organelle interaction in eukaryotic cells, in which various organelles or proteins are recycled and operated through the autophagy pathway to ensure nutrient and energy homeostasis. Although numerous fluorescent probes have been developed to image autophagy, these environment‐responsive probes suffer from inherent deficiencies such as inaccuracy and limited versatility. Here, we present a modular macrocyclic amphiphile Förster Resonance Energy Transfer (FRET) platform (SC6A12C/NCM, SN), constructed through the amphiphilic assembly of sulfonatocalix[6]arene (SC6A12C) with N‐cetylmorpholine (NCM) for lysosome targeting. The hydrophobic fluorophore BPEA (FRET donor) was entrapped within the inner hydrophobic phase and showed strong fluorescence emission. Attributed to the broad‐spectrum encapsulation of SC6A12C, three commercially available organelle probes (Mito‐Tracker Red, ER Tracker Red, and RhoNox‐1) were selected as SC6A12C guests (FRET acceptors). During autophagy process, the formation of intracellular host‐guest complexes leads to strong FRET signal, allowing us to visualize the fusion of mitochondria, endoplasmic reticulum, and Golgi apparatus with lysosomes, respectively. This study provides a versatile and accessible platform for imaging organelle autophagy.

show abstract

A FRET Autophagy Imaging Platform by Macrocyclic Amphiphile

Jiang,

Chen,

Chen

et al. 2024

Angew Chem Int Ed

0

View full text Add to dashboard Cite

Autophagy is a ubiquitous process of organelle interaction in eukaryotic cells, in which various organelles or proteins are recycled and operated through the autophagy pathway to ensure nutrient and energy homeostasis. Although numerous fluorescent probes have been developed to image autophagy, these environment‐responsive probes suffer from inherent deficiencies such as inaccuracy and limited versatility. Here, we present a modular macrocyclic amphiphile Förster Resonance Energy Transfer (FRET) platform (SC6A12C/NCM, SN), constructed through the amphiphilic assembly of sulfonatocalix[6]arene (SC6A12C) with N‐cetylmorpholine (NCM) for lysosome targeting. The hydrophobic fluorophore BPEA (FRET donor) was entrapped within the inner hydrophobic phase and showed strong fluorescence emission. Attributed to the broad‐spectrum encapsulation of SC6A12C, three commercially available organelle probes (Mito‐Tracker Red, ER Tracker Red, and RhoNox‐1) were selected as SC6A12C guests (FRET acceptors). During autophagy process, the formation of intracellular host‐guest complexes leads to strong FRET signal, allowing us to visualize the fusion of mitochondria, endoplasmic reticulum, and Golgi apparatus with lysosomes, respectively. This study provides a versatile and accessible platform for imaging organelle autophagy.

show abstract

A lysosome-targeted fluorescent probe based on a BODIPY structure for Cys/Hcy detection

Abstract: Cysteine (Cys) and Homocysteine (Hcy) are important biothiols in living organisms. They play important roles in a variety of physiological and pathological processes. Therefore, it is very important to design...

Cited by 8 publications

References 45 publications

A lateral flow strip for on-site detection of homocysteine based on a truncated aptamer

A lateral flow strip for on-site detection of homocysteine based on a truncated aptamer

A FRET Autophagy Imaging Platform by Macrocyclic Amphiphile

A FRET Autophagy Imaging Platform by Macrocyclic Amphiphile

Contact Info

Product

Resources

About