“…26,27 Orthogonal experimental design (OED), as a common method to test the effectiveness of multi-factor conditions, has been used to evaluate multi-level factors and select the best combination to achieve rapid screening and optimization of experimental parameters through relatively few experiments, 28 making it an efficient, fast and economical experimental design method. 29,30 Further, in the SERS detection process, the spectral signals of uorescent substances in food and agricultural products can seriously weaken the Raman characteristics of analytes. Therefore, the uorescent substances must be eliminated before the Raman analysis is performed, to improve the accuracy for detection of the analyte.…”