A macroscopic and stereological imaging dataset of Pleuronectes platessa ovaries

Abstract

the North Sea plaice, Pleuronectes platessa (Linnaeus, 1758), is a commonly studied commercial flatfish with poorly known ovarian histology. The following dataset is a collection of female plaice gonad images and their corresponding histological slides, collected during a complete season of the plaice's reproduction cycle. Stereology was used to determine the percentage of different structures found throughout the ovaries. Inter-agent calibrations were accomplished in order to harmonize the stereological readings, and were based on a comprehensive reading protocol and histological lexicon that were specifically written for the plaice's ovaries. The distribution and homogeneity of the different cell types found throughout the ovaries were also evaluated. This dataset can be used to automate the stereological reading process (through statistical learning methods for example) or to objectively determine the plaice's maturity phase, and link that information to either macroscopic measurements or through image analysis of the full ovaries.

A Quantitative Histologic Analysis of Oogenesis in the Flatfish Species Pleuronectes platessa as a Tool for Fisheries Management

“…The aim of this study was to establish an accurate description of the oogenesis cycle for this pleuronectid as well as underline the main criteria linked to the spawning period of mature individuals. For this, a histological approach, enhanced through the use of stereology, was used [19].…”

“…The procedure for quantitative analysis was previously described [19]. Using a histology slide scanner (Aperio CS, Scan Scope Console software, v.10.2.0.2352), the slides were scanned and analyzed with the Aperio software (v12.1.0.5029).…”

“…Inter-agent calibration with a reading protocol [22] was used to validate this stereological approach. Cellular homogeneity inter-gonad (ventral and dorsal) and intra-gonad (anterior median and posterior sections) was also checked [19]. Once cellular homogeneity was demonstrated, the reading results of the ventral ovary were used.…”