A mannose binding protein is involved in the adherence of<i>Acanthamoeba</i>species to inert surfaces

Imbert-Bouyer, Sabrina; Merlaud, Annabelle; Imbert, Christophe; Daniault, Gyslaine; Rodier, Marie-HÃ©lÃ ̈ne

doi:10.1111/j.1574-6968.2004.tb09757.x

Cited by 9 publications

(3 citation statements)

References 14 publications

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“…The reproducibility of results from testing cysts in particular has been described to be difficult [ 9 ]. Part of the variability within test replications is probably caused by trophozoites and cysts sticking to plastic surfaces when preparing solutions and dilutions [ 24 ]. We therefore performed all experiments in triplicate.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Effectiveness of Soft Contact Lens Solutions Available on the Dutch Market against Acanthamoeba Species

Randag

Kroon²,

Otten³

et al. 2023

Pathogens

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Acanthamoeba keratitis is almost universally associated with contact lens (CL) use. Until today, however, CL solution manufacturing protocols lack testing of anti-amoebic activity. This study investigates the effectiveness of CL solutions available on the Dutch market against trophozoites and cysts of Acanthamoeba castellanii and Acanthamoeba polyphaga. Sixteen CL solutions were tested: 13 multiple purpose solutions (MPS), 2 hydrogen peroxidase solutions (HPS) and 1 povidone-iodine-based solution (PIS). The Spearman–Karber (SK) log reduction method and an XTT colorimetric assay were used to evaluate the effectiveness at the manufacturer’s minimum recommended disinfection time (MMRDT) and after eight hours. At the MMRDT, one MPS showed an SK mean log reduction (MLR) of >3.0 against A. castellanii trophozoites. Two additional MPS and both HPS reached this threshold after eight hours. The SK MLR values for A. polyphaga trophozoites were between 1 and 3 at all time points. Using the XTT colorimetric assay, only HPS 1 showed >99.9% reduction (equivalent to 3 log reduction) in metabolic activity of A. castellanii trophozoites after eight hours. For A. polyphaga, both HPS and PIS showed a metabolic reduction of >99.9% after eight hours. Cysts were resistant against all solutions. We conclude that following the manufacturer’s guidelines, few solutions provide sufficient effectiveness against Acanthamoeba trophozoites and none against cysts. The results underline the importance of adequate hygiene when handling CLs.

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Section: Discussionmentioning

confidence: 99%

In Vitro Effectiveness of Soft Contact Lens Solutions Available on the Dutch Market against Acanthamoeba Species

Randag

Kroon²,

Otten³

et al. 2023

Pathogens

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“…Polyclonal and monoclonal antibodies may be useful tools for identifying and characterizing both specific and conserved components in different Acanthamoeba strains and indicate the presence of molecules associated or not with pathogenicity (Imbert-Bouyer et al 2004; Garate et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Physiological, morphological, and immunochemical parameters used for the characterization of clinical and environmental isolates ofAcanthamoeba

Becker-Finco

Costa²,

Silva

et al. 2012

Parasitology

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The factors that characterize Acanthamoeba strains as harmless or potentially pathogenic have not been elucidated. Analysing the in vitro and in vivo parameters of Acanthamoeba samples, including heat tolerance at temperatures close to that of the human body, cytopathic effects, and their ability to cause infections in animals, has been proposed to identify their pathogenic potential. Another promising criterion for differentiating strains is the analysis of their biochemical and immunochemical properties. In this study, a comparative evaluation between clinical and environmental Acanthamoeba isolates was performed on the basis of physiological, morphological, and immunochemical criteria. Crude antigens were used to characterize the protein profiles by electrophoresis and immunize mice to produce polyclonal and monoclonal antibodies. The antibodies were characterized using ELISA, Western blotting, and immunofluorescence techniques. The results obtained with polyclonal antibodies suggest the presence of specific proteins for each studied isolate and co-reactive immunochemical profiles among conserved components. Ten monoclonal antibody clones were obtained; mAb3 recognized 3 out of 4 samples studied. The results of this study may help standardize criteria for identifying and characterizing Acanthamoeba strains. Taken together, our results support the view that a set of features may help differentiate Acanthamoeba species and isolates.

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“…The reason for discrepancies in results from different published reports regarding the efficacy of disinfecting solution for SCLs against Acanthamoeba may be attributed to the lack of established objective standard methods, including strain adjustment. To prevent the variability in results because of trophozoites and cysts adhering to plastic surfaces during strain preparation, 57 Randag et al 51 conducted all experiments in triplicate. Although for A. castellanii 50370 trophozoites, PVP-I (cleadew), HP system (Easysept and Avizor Everclean), and three types of MPDS (Kruidavat Opticare, Revi-taLens Ocutec, and OPTI-FREE Puremoist) (Table 1) exhibited significant disinfecting efficacy of more than a 3 log reduction, for A. castellanii 50370 cysts, A. polyphaga 30461 trophozoites, and cysts, these solutions did not reach more than a 3 log reduction.…”

Section: Anti-acanthamoeba Properties Of Povidone-iodine Solutionmentioning

confidence: 99%

Advances in Contact Lens Care Solutions: PVP-I Disinfectant and HAD Wetting Agents From Japan

Itokawa,

Yamasaki,

Suzuki

et al. 2023

Eye &Amp; Contact Lens: Science &Amp; Clinical Practice

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Half of the individuals who wear contact lenses use reusable lenses that require proper care. Improper contact lens (CL) care and using inadequate disinfecting solutions can lead to lens contamination, CL-related microbial keratitis, and Acanthamoeba keratitis. Oxidative disinfecting solutions, such as hydrogen peroxide, show higher efficacy than multipurpose solutions. Povidone–iodine (PVP-I), an oxidative disinfectant used in ophthalmic surgery, has been proven to be safe and effective. The PVP-I system, a CL disinfecting solution developed in Japan, has demonstrated excellent antimicrobial and antiviral properties. Although CL discomfort does not have a risk of ocular disorders with poor visual prognosis, such as keratitis, CL discomfort can still lead to lens dropout and thus needs to be addressed. To mitigate CL discomfort, it is essential to use disinfecting solutions containing surfactants and wetting agents that improve wettability of the lens surface. A CL solution containing hyaluronic acid derivatives (HADs) as wetting agents that permanently adhere to the lens surface to improve wettability of the lens surface was developed in Japan. There is potential for HAD to be integrated into various solutions. This article reviews the efficacy of novel PVP-I–based disinfecting solution and HAD wetting agents.

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A mannose binding protein is involved in the adherence ofAcanthamoebaspecies to inert surfaces

Cited by 9 publications

References 14 publications

In Vitro Effectiveness of Soft Contact Lens Solutions Available on the Dutch Market against Acanthamoeba Species

In Vitro Effectiveness of Soft Contact Lens Solutions Available on the Dutch Market against Acanthamoeba Species

Physiological, morphological, and immunochemical parameters used for the characterization of clinical and environmental isolates ofAcanthamoeba

Advances in Contact Lens Care Solutions: PVP-I Disinfectant and HAD Wetting Agents From Japan

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