A mannose receptor is involved in the anti-Vibrio defense of red swamp crayfish

Man, Xin; Pan, Xin-Tong; Zhang, Hongwei; Wang, Yue; Li, Xin-Cang; Zhang, Xiaowen

doi:10.1016/j.fsi.2018.08.021

Cited by 20 publications

(9 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with that reported in the PcMR of P. clarkii [ 11 ], LvMR was predominantly expressed in the hepatopancreas of L. vannamei . The hepatopancreas is not only a digestive gland, but also an important organ involved in the immune response of crustaceans [ 20 , 21 ].…”

Section: Discussionsupporting

confidence: 90%

“…Different from the vertebrate MRs, LvMR had only the FN II domain and two CRDs. This is similar to the reported PcMR in P. clarkii [ 11 ] and other invertebrate MRs available in GenBank, such as MRs from H. azteca , C. gigas and Lingula anatina , which had 12–15 CRDs with the absence of CR and FNII domains. The structural differences indicate that the domains of MRs are not conserved in invertebrates.…”

Section: Discussionsupporting

confidence: 89%

“…The rLvMR protein agglutinated bacteria and the fungus in the presence of Ca 2+ , indicating that LvMR is a member of the Ca 2+ -dependent mannose receptors. The similar microorganism agglutination has been found in other reported MRs. For example, in the presence of Ca 2+ , the rCTLD1-3 and rCTLD11-14 of PcMR could agglutinate most test bacteria and fungi [ 11 ], and rOnMR could agglutinate Streptococcus agalactiae and Aeromonas hydrophila [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

“…Blunt snout bream MR was reported to be involved in the phagocytosis of macrophage to bacteria, accompanied by respiratory burst, nitric oxide (NO) production and inflammatory cytokines secretion [ 8 ]. To date, invertebrate MR has only been identified in the red swamp crayfish Procambarus clarkii , which contained 14 CTLDs and a transmembrane domain [ 11 ]. Knowledge about the structure and function of MRs in invertebrates is very limited.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A Mannose Receptor from Litopenaeus vannamei Involved in Innate Immunity by Pathogen Recognition and Inflammation Regulation

Guo

Liu

Hao

et al. 2023

IJMS

View full text Add to dashboard Cite

Mannose receptor, as a member of the C-type lectin superfamily, is a non-canonical pattern recognition receptor that can internalize pathogen-associated ligands and activate intracellular signaling. Here, a mannose receptor gene, LvMR, was identified from the Pacific white shrimp Litopenaeus vannamei. LvMR encoded a signal peptide, a fibronectin type II (FN II) domain, and two carbohydrate-recognition domains (CRDs) with special EPS and FND motifs. LvMR transcripts were mainly detected in the hepatopancreas, and presented a time-dependent response after pathogen challenge. The recombinant LvMR (rLvMR) could bind to various PAMPs and agglutinate microorganisms in a Ca2+-dependent manner with strong binding ability to D-mannose and N-acetyl sugars. The knockdown of LvMR enhanced the expression of most NF-κB pathway genes, inflammation and redox genes, while it had no obvious effect on the transcription of most phagocytosis genes. Moreover, the knockdown of LvMR caused an increase in reactive oxygen species (ROS) content and inducible nitric oxide synthase (iNOS) activity in the hepatopancreas after Vibrio parahaemolyticus infection. All these results indicate that LvMR might perform as a PRR in immune recognition and a negative regulator of inflammation during bacterial infection.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Mannose Receptor from Litopenaeus vannamei Involved in Innate Immunity by Pathogen Recognition and Inflammation Regulation

Guo

Liu

Hao

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Contrary to the results obtained for Mr(T)Lec2, the C-type lectin transcripts (C-type; dual carbohydrate recognition domain type) of M. rosenbergii challenged with V. anguillarum showed an upregulation (Ren et al, 2012). Further, a mannose-receptor was found to be induced upon V. anguillarum infection in red swamp crayfish, Procambrus clarkii (Man et al, 2018). In this context, it will be interesting to note the promiscuity of Mr(T)Lec4 and Mr(T)Lec2 identified in this study with homologous sequences available in the databases.…”

Section: Mr(t)lec4contrasting

confidence: 83%