A mechanical evaluation of three decellularization methods in the design of a xenogeneic scaffold for tissue engineering the temporomandibular joint disc

Lumpkins, Sarah B.; Pierre, Nicolas; McFetridge, Peter S.

doi:10.1016/j.actbio.2008.01.016

Cited by 151 publications

(101 citation statements)

References 40 publications

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“…There is no study in decellularizing allogenic or xenogenic nucleus or annulus for replacement at all. Nevertheless, there is one study evaluating the mechanical properties of decellularized temporomandibular joint disc [73]. Although the immune privileged status of IVD, which is avascular, allows the application of allografts without decellularization, research efforts in decellularizing nucleus and annulus grafts from xenogenic sources for scaffolding should be encouraged because allografts are not always available.…”

Section: Scaffolding In Intervertebral Disc Tissue Engineeringmentioning

confidence: 99%

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

2008

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Scaffolds represent important components for tissue engineering. However, researchers often encounter an enormous variety of choices when selecting scaffolds for tissue engineering. This paper aims to review the functions of scaffolds and the major scaffolding approaches as important guidelines for selecting scaffolds and discuss the tissue-specific considerations for scaffolding, using intervertebral disc as an example.

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Section: Scaffolding In Intervertebral Disc Tissue Engineeringmentioning

confidence: 99%

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

2008

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“…SDS, as a decellularization agent, seems to be more effective than Triton X-100 since its effectiveness is higher, especially with dense organs such as kidney, liver or temporomandibular joints [30][31][32] . On the other hand, SDS has deleterious effects upon the amount of GAG in the ECM.…”

Section: Non-ionic Detergentsmentioning

confidence: 99%

Chemical decellularization: a promising approach for preparation of extracellular matrix

Hrebíková¹,

Díaz²,

Mokrý³

2015

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Background.A biological scaffold from extracellular matrix can be produced by a variety of decellularization methods whose caveat consists in efficiently eliminating cells from the treated tissue. This scaffold can be used in diverse applications for tissue engineering and organ regeneration. Preservation of the extracellular matrix ultrastructure is highly desirable because of its unique architecture, contained growth factors and decreased immunological response. All of these properties provide attachment sites and adequate environment for cells colonizing this scaffold, reconstituting the decellularized organ. This review briefly describes chemical decellularization methods, evaluation of these protocols and the role of ECM in tissue engineering. Conclusion. Chemical decellularization is an often used method for scaffold preparation and makes possible a wellpreserved three dimensional structure of extracellular matrix.

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“…This hypothesis is supported by the pattern of cellular depletion we noted in lymph nodes treated with CHAPS or Triton-X (i.e., mostly depleted in the cortex) as well as previous studies demonstrating that SDS is more effective than Triton X-100 for removing nuclei from dense tissues and organs. 8,24,25 An alternative method to achieve decellularization may be in vivo perfusion with detergents thus enabling bypass of the lymph node capsule. 9,20 However, this method is likely less clinically applicable due to potential difficulties in perfusionfixation of human donor tissues.…”

Section: Discussionmentioning

confidence: 99%

Decellularized Lymph Nodes as Scaffolds for Tissue Engineered Lymph Nodes

Cuzzone

Albano

Aschen

et al. 2015

Lymphatic Research and Biology

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Background: The lymphatic system is commonly injured during cancer treatment. However, despite the morbidity of these injuries, there are currently no options for replacing damaged lymphatics. The purpose of this study was to optimize methods for decellularization of murine lymph nodes (LN) and to determine if these scaffolds can be used to tissue engineer lymph node-like structures. Methods and Results: LNs were harvested from adult mice and subjected to various decellularization protocols. The degree of decellularization and removal of nuclear material was analyzed histologically and quantitatively using DNA isolation. In addition, we analyzed histological architecture by staining for matrix proteins. After the optimal method of decellularization was identified, decellularized constructs were implanted in the renal capsule of syngeneic or allogeneic recipient mice and analyzed for antigenicity. Finally, to determine if decellularized constructs could deliver lymphocytes to recipient animals, the matrices were repopulated with splenocytes, implanted in submuscular pockets, and harvested 14 days later. Decellularization was best accomplished with the detergent sodium dodecyl sulfate (SDS), resulting in negligible residual cellular material but maintenance of LN architecture. Implantation of decellularized LNs into syngeneic or allogeneic mice did not elicit a significant antigenic response. In addition, repopulation of decellularized LNs with splenocytes resulted in successful in vivo cellular delivery. Conclusions: We show, for the first time, that LNs can be successfully decellularized and that these matrices have preserved extracellular matrix architecture and the potential to deliver leukocytes in vivo. Future studies are needed to determine if tissue engineered lymph nodes maintain immunologic function.

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A mechanical evaluation of three decellularization methods in the design of a xenogeneic scaffold for tissue engineering the temporomandibular joint disc

Cited by 151 publications

References 40 publications

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

Scaffolding in tissue engineering: general approaches and tissue-specific considerations

Chemical decellularization: a promising approach for preparation of extracellular matrix

Decellularized Lymph Nodes as Scaffolds for Tissue Engineered Lymph Nodes

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