A mediator-free glucose biosensor based on glucose oxidase/chitosan/α-zirconium phosphate ternary biocomposite

Liu, Limin; Wen, Jiwu; Liu, Lijun; He, Deyong; Kuang, Ren‐Yun; Shi, Taqing

doi:10.1016/j.ab.2013.10.005

Cited by 24 publications

(9 citation statements)

References 45 publications

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“…In total: The 3D-KSC/GOD electrode could be used to detect glucose based on the changes of O 2 reduction peak current. 47 4B, the cathodic peak current decreased linearly with the increase of glucose concentration from 0.1 to 14.0 mM with a sensitivity of 1.73 μA mM −1 . The detection limit was estimated to be about 50.75 μM based on the criterion of a signal-to-noise ratio of 3.…”

Section: Electrocatalytic Properties Of 3d-ksc/god Electrodesmentioning

confidence: 94%

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A glucose biosensor based on glucose oxidase immobilized on three-dimensional porous carbon electrodes

Chen

Zhu

Huang

et al. 2015

Analyst

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A novel glucose biosensor was developed by immobilizing glucose oxidase (GOD) on a three-dimensional (3D) porous kenaf stem-derived carbon (3D-KSC) which was firstly proposed as a novel supporting material to load biomolecules for electrochemical biosensing. Here, an integrated 3D-KSC electrode was prepared by using a whole piece of 3D-KSC to load the GOD molecules for glucose biosensing. The morphologies of integrated 3D-KSC and 3D-KSC/GOD electrodes were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The SEM results revealed a 3D honeycomb macroporous structure of the integrated 3D-KSC electrode. The TEM results showed some microporosities and defects in the 3D-KSC electrode. The electrochemical behaviors and electrocatalytic performance of the integrated 3D-KSC/GOD electrode were evaluated by cyclic voltammetry and electrochemical impedance spectroscopy. The effects of pH and scan rates on the electrochemical response of the biosensor have been studied in detail. The glucose biosensor showed a wide linear range from 0.1 mM to 14.0 mM with a high sensitivity of 1.73 μA mM(-1) and a low detection limit of 50.75 μM. Furthermore, the glucose biosensor exhibited high selectivity, good repeatability and reproducibility, and good stability.

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Section: Electrocatalytic Properties Of 3d-ksc/god Electrodesmentioning

confidence: 94%

“…The 3D-KSC/GOD electrode could be used to detect glucose based on the changes of O 2 reduction peak current 47 . To exhibit the advantages of proposed biosensor, the analytical performances of developed biosensor were compared with other glucose sensors in Table 1.…”

Section: Electrochemical Behaviors Of 3d-ksc/god Electrodesmentioning

confidence: 99%

A glucose biosensor based on glucose oxidase immobilized on three-dimensional porous carbon electrodes

Chen

Zhu

Huang

et al. 2015

Analyst

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“…Cryogel is a form of a gel formed by freeze-drying a sol. The porous structure of cryogel provides a large surface area for biomolecule immobilization, which also has a significant impact on enzyme activity and reusability [71][72][73]. The parameters including CS polymer ratio, amount of cross-linker, the temperature of the cryogelation process, and stirring conditions would directly influence the resulting gel s chemistry, pore morphology, microsphere size, swelling behavior, and degradation rate [74][75][76][77][78][79][80].…”

Section: Chitosan Cryogelmentioning

confidence: 99%

Exploration of Chitinous Scaffold-Based Interfaces for Glucose Sensing Assemblies

Bagal-Kestwal

Chiang

2019

Polymers

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The nanomaterial-integrated chitinous polymers have promoted the technological advancements in personal health care apparatus, particularly for enzyme-based devices like the glucometer. Chitin and chitosan, being natural biopolymers, have attracted great attention in the field of biocatalysts engineering. Their remarkable tunable properties have been explored for enhancing enzyme performance and biosensor advancements. Currently, incorporation of nanomaterials in chitin and chitosan-based biosensors are also widely exploited for enzyme stability and interference-free detection. Therefore, in this review, we focus on various innovative multi-faceted strategies used for the fabrication of biological assemblies using chitinous biomaterial interface. We aim to summarize the current development on chitin/chitosan and their nano-architecture scaffolds for interdisciplinary biosensor research, especially for analytes like glucose. This review article will be useful for understanding the overall multifunctional aspects and progress of chitin and chitosan-based polysaccharides in the food, biomedical, pharmaceutical, environmental, and other diverse applications.

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“…Several useful applications utilizing α -ZrP have been tested including drug delivery, 15,16 catalysis, 17 ion exchange, 18 and glucose sensing. 19 Furthermore, specific metal ions were successfully used to continuously tune the binding affinity of anionic GOx with the anionic α -ZrP nanosheets, 11 revealing that metal ions that have favorable interactions with the enzyme/solid increased the binding affinities the most. For example, GOx/ α -ZrP affinity increased 380-fold in the presence of Zr(IV) and Ca(II) ions, due to their affinities for the phosphate groups of the nanosheets.…”

Section: Introductionmentioning

confidence: 99%

Tuning Enzyme/α-Zr(IV) Phosphate Nanoplate Interactions via Chemical Modification of Glucose Oxidase

et al. 2017

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Using glucose oxidase (GOx) and α-Zr(IV) phosphate nanoplates (α-ZrP) as a model system, a generally applicable approach to control enzyme–solid interactions via chemical modification of amino acid side chains of the enzyme is demonstrated. Net charge on GOx was systematically tuned by appending different amounts of polyamine to the protein surface to produce chemically modified GOx(n), where n is the net charge on the enzyme after the modification and ranged from −62 to +95 electrostatic units in the system. The binding of GOx(n) with α-ZrP nanosheets was studied by isothermal titration calorimetry (ITC) as well as by surface plasmon resonance (SPR) spectroscopy. Pristine GOx showed no affinity for the α-ZrP nanosheets, but GOx(n) where n ≥ −20 showed binding affinities exceeding (2.1 ± 0.6) × 106 M−1, resulting from the charge modification of the enzyme. A plot of GOx(n) charge vs Gibbs free energy of binding (ΔG) for n = +20 to n = +65 indicated an overall increase in favorable interaction between GOx(n) and α-ZrP nanosheets. However, ΔG is less dependent on the net charge for n > +45, as evidenced by the decrease in the slope as charge increased further. All modified enzyme samples and enzyme/α-ZrP complexes retained a significant amount of folding structure (examined by circular dichroism) as well as enzymatic activities. Thus, strong control over enzyme–nanosheet interactions via modulating the net charge of enzymes may find potential applications in biosensing and biocatalysis.

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A mediator-free glucose biosensor based on glucose oxidase/chitosan/α-zirconium phosphate ternary biocomposite

Cited by 24 publications

References 45 publications

A glucose biosensor based on glucose oxidase immobilized on three-dimensional porous carbon electrodes

A glucose biosensor based on glucose oxidase immobilized on three-dimensional porous carbon electrodes

Exploration of Chitinous Scaffold-Based Interfaces for Glucose Sensing Assemblies

Tuning Enzyme/α-Zr(IV) Phosphate Nanoplate Interactions via Chemical Modification of Glucose Oxidase

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