A membrane-associated adenylate cyclase modulates lactate dehydrogenase and creatine kinase activities required for bull sperm capacitation induced by hyaluronic acid

Fernandez, Sebastian P.; Córdoba, Mariana

doi:10.1016/j.anireprosci.2017.02.004

Cited by 5 publications

(3 citation statements)

References 51 publications

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“…Regarding the effects of different sperm separation methods on the pregnancy rate of IVF, it has been suggested that there are no significant differences between the CSW and DGC methods (as shown in Table 2 ). 44 , 48 , 49 However, many studies have reported that the other two sorting methods, MACS and HA, achieve significantly better sperm quality than CSW and DGC, despite some disadvantages. There are limited data available on microfluidic sperm separation, and there is still a lack of unified statistical analyses of clinical data on the pregnancy rate when using these different sperm sorting methods.…”

Section: Discussionmentioning

confidence: 99%

“…[42][43][44] Additionally, it remains unclear whether abnormal apoptotic processes start before or after ejaculation, inducing apoptotic markers expression in spermatozoa. [45][46][47][48][49][50][51][52][53][54][55][56][57] Therefore, it is essential to further assess the sperm morphology index in nonapoptotic and apoptotic sperm. Simultaneously, the effects of magnetic forces on sperm quality are understudied and require further investigation.…”

Section: Magnetic Activated Cell Sortingmentioning

confidence: 99%

“…It has been reported that HA is linked with several signaling pathways and induces sperm capacitation and acrosome reaction. 48 During fertilization, the combination of HA and sperm influences sperm motility and facilitates sperm penetration, promoting sperm nuclear maturation, plasma membrane remodeling, and acrosome extrusion. 49 , 50 Only mature sperm express the receptors for HA, which allow them to bind to the oocyte for fertilization.…”

Section: Hyaluronic Acidmentioning

confidence: 99%

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Effects of sperm processing techniques on IVF pregnancy rates: a mini-review

Zhao¹,

Sun²,

Zhao

2023

Clin Med�Insights�Reprod�Health

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Many factors associated with assisted reproductive technologies significantly influence the success of pregnancy after in vitro fertilization (IVF) either directly or indirectly. These factors include sperm processing techniques, egg retrieval, intrauterine artificial insemination, intracytoplasmic sperm injection, and embryo transfer. Among these technologies, sperm quality is one of the most critical factors for a successful IVF pregnancy. The method used for sperm processing plays a crucial role in determining the quality of sperm. Several widely used sorting techniques, such as conventional swim-up, density gradient centrifugation, magnetic activated cell sorting, and hyaluronic acid, have been extensively compared in various studies. Previous studies have shown that each sperm processing method causes varying degrees of sperm damage, particularly in sperm motility, concentration, morphological features, viability, and DNA integrity. However, sperm processing techniques have been developed slowly, and the impact of these methods on pregnancy rates is still unclear. Further exploration is needed. In this review, we aim to compare the results of different sperm processing techniques concerning sperm quality and IVF pregnancy rates. We will also discuss possible clinical approaches, such as microfluidics and integrated approaches, for testing and improving sperm quality.

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Section: Discussionmentioning

confidence: 99%