A meta cleavage pathway for 4-chlorobenzoate, an intermediate in the metabolism of 4-chlorobiphenyl by Pseudomonas cepacia P166

Arensdorf, Joseph J.; Focht, D. D.

doi:10.1128/aem.61.2.443-447.1995

Cited by 84 publications

(41 citation statements)

References 24 publications

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“…Burkholderia cepacia P166, which possesses meta-cleavage activity with 4-chlorocatechols (CCs), degrades 4-chlorobiphenyl and 4-chlorobenzoate without product inhibition, unlike its degradation of 3-chlorobiphenyl (Arensdorf and Focht 1995) (Fig. 2).…”

Section: Resultsmentioning

confidence: 99%

Efficiency of chlorocatechol metabolism in natural and constructed chlorobenzoate and chlorobiphenyl degraders

et al. 2004

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Aims: A possibility for the complementation of both ortho-and meta-cleavage pathway for chlorocatechols in one strain and its impact on degradation of chlorobenzoates accumulated during degradation of polychlorinated biphenyls was investigated. Methods and Results: Genes responsible for ortho-cleavage of chlorocatechols were subcloned into two biphenyl degraders and the activities of chlorocatechol dioxygenases responsible for ortho-and meta-cleavage in these hybrid strains were monitored spectrophotometrically and also electrochemically by ion-selective electrode. Conclusions: While strain Pseudomonas fluorescens S12/C apparently gained metabolic advantage from this gene manipulation, strain Burkholderia cepacia P166/C did not express better degradation features in comparison with the parental strain. Significance and Impact of the Study: This approach has the potential to enhance chlorocatechol metabolism in selected biphenyl degraders.

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Section: Resultsmentioning

confidence: 99%

Efficiency of chlorocatechol metabolism in natural and constructed chlorobenzoate and chlorobiphenyl degraders

et al. 2004

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“…In contrast, 2,3-extradiol cleavage of 3-CC leads to lethal production of 5-chloroformyl-2-hydroxypenta-2,4-dienoic acid, which is a potent inhibitor of meta-pyrocatechases [17]. In contrast, meta-¢ssion of 4-CC does not generate an acyl halide [4,5]. Wild-type strain M3GY could mineralize 3,4P-DCBP exclusively through the meta-¢ssion pathway if the initial dioxygenation occurred only on the meta substituted ring.…”

Section: Discussionmentioning

confidence: 98%

“…Cells were grown in chloride-free mineral salts media (MSM) which has been previously described [5]. MSM plates were prepared by adding Noble agar at 1.7%.…”

Section: Mediamentioning

confidence: 99%

“…Chloroacetate was obtained from Kodak Chemical Co. (Rochester, NY, USA) and was recrystallized twice from dichloromethane before use. Diazomethane was generated by alkali decomposition of N-nitroso-N-methylurea, Sigma Chemical Co. (St. Louis, MO, USA) [5]. 3,4P-DCBP was purchased from Accustandard (New Haven, CN, USA).…”

Section: Chemicalsmentioning

confidence: 99%

“…HPLC (high-pressure liquid chromatography) was used for detection of chlorobenzoates and performed with a System Gold model 126 pump and model 166 UV detector (both from Beckman Instruments, Inc., Fullerton, CA, USA) as previously described [5].…”

Section: Analyticalmentioning

confidence: 99%

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The use of mutants to discern the degradation pathway of 3,4′-dichlorobiphenyl in Pseudomonas acidovorans M3GY

Cullar¹

2002

FEMS Microbiology Ecology

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Pseudomonas acidovorans strain M3GY is a recombinant bacterium with the novel ability to utilize 3,4P-dichlorobiphenyl (3,4P-DCBP) as a growth substrate. This strain was previously shown to oxidize the 3P-ring and produce 4-chlorobenzoate (4-CBa) through the standard biphenyl pathway. Although 4-CBa was metabolized through the meta-fission pathway, the genes encoding the orthochlorocatechol pathway were retained. Nevertheless, neither 3-CBa nor 3-chlorocatechol (3-CC) were detected as intermediates during metabolism of 3,4P-DCBP, nor was 4-CBa utilized as a sole carbon source, by this strain. Two mutant strains were produced to resolve these anomalies. Mutant strain M3GY-9 was obtained by Tn5 insertion and selection for growth on biphenyl, and was unable to grow on 3-CBa. It accumulated 3-CC from 3,4P-DCBP when grown on biphenyl. Thus, M3GY attacks both rings, and the failure to isolate 3-CBa or 3-CC is due to rapid turnover by the enzymes of the ortho-chlorocatechol pathway in the wild-type strain. Mutant strain M3GY-1 grew on 4-CBa, unlike the wild-type strain. Washed cell suspensions of mutant strain MEGY-1 consumed 4-fluorobenzoate, 4-bromobenzoate, and, to a lesser extent 4-iodobenzoate. The mutation that resulted in the ability of mutant strain M3GY-I to effectively utilize 4-CBa as a sole carbon source was associated with a transport mechanism. ß 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.

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Burkholderia

Palleroni¹

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Burk.hol.de' ri.a . M.L. fem. n. Burkholderia named after W.H. Burkholder, American bacteriologist who discovered the etiological agent of onion rot. Proteobacteria / Betaproteobacteria / Burkholderiales / Burkholderiaceae / Burkholderia Cells single or in pairs, straight or curved rods , but not helical. Dimensions, generally 0.5–1 × 1.5–4 µm. Motile by means of one or, more commonly, several polar flagella . One species ( Burkholderia mallei ) lacks flagella and is nonmotile. Do not produce sheaths or prosthecae. No resting stages are known. Gram negative. Most species accumulate poly‐β‐hydroxybutyrate (PHB) as carbon reserve material . Chemoorganotrophs. Have a strictly respiratory type of metabolism with oxygen as the terminal electron acceptor . Some species can exhibit anaerobic respiration with nitrate. Strains of some of the species ( B. cepacia , B. vietnamiensis ) are able to fix N 2 . Catalase positive. A wide variety of organic compounds can be used as sources of carbon and energy for growth. Although hydroxylated fatty acids are present in the lipids of members of other genera of aerobic pseudomonads, species of Burkholderia are characterized by the presence of hydroxy fatty acids of 14, 16, and 18 carbon atoms (C 14:0 3OH and C 16:0 , and C 16:0 2OH , C 16:1 , and C 18:1 ) . The most characteristic of these acids is the C 16:0 3OH . Two different ornithine lipids are present in strains of some of the species. Over one‐half of the species are pathogenic for plants or animals (including humans) . The genus belongs to the ribosomal RNA similarity group II, which can be differentiated from other groups of aerobic pseudomonads by rRNA/DNA hybridization experiments or by rDNA sequencing. The mol% G + C of the DNA is : 59–69.6. Type species : Burkholderia cepacia (Palleroni and Holmes 1981) Yabuuchi, Kosako, Oyaizu, Yano, Hotta, Hashimoto, Ezaki and Arakawa 1993, 398 (Effective publication: Yabuuchi, Kosako, Oyaizu, Yano, Hotta, Hashimoto, Ezaki and Arakawa 1992, 1271) ( Pseudomonas cepacia Palleroni and Holmes 1981, 479.)

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A meta cleavage pathway for 4-chlorobenzoate, an intermediate in the metabolism of 4-chlorobiphenyl by Pseudomonas cepacia P166

Cited by 84 publications

References 24 publications

Efficiency of chlorocatechol metabolism in natural and constructed chlorobenzoate and chlorobiphenyl degraders

Efficiency of chlorocatechol metabolism in natural and constructed chlorobenzoate and chlorobiphenyl degraders

The use of mutants to discern the degradation pathway of 3,4′-dichlorobiphenyl in Pseudomonas acidovorans M3GY

Burkholderia

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