A metabolic study to decipher amino acid catabolism-directed biofuel synthesis in Acetoanaerobium sticklandii DSM 519

Sangavai, C.; Chellapandi, P.

doi:10.1007/s00726-019-02777-4

Cited by 6 publications

(3 citation statements)

References 58 publications

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“…All Terrisporobacter isolates shared a common growth pattern in H 2 + CO 2 -supplemented cultures. Initial growth took place by consuming nutrients provided by the complex substrates peptone and yeast extract in the medium, likely by performing the acetate-producing pairwise fermentation of amino acids via the Stickland reaction ( Stickland, 1935 ; Sangavai and Chellapandi, 2019 ). The trace amounts of other products besides acetate and ethanol detected by the GC-measurements likely originated from these Stickland reactions.…”

Section: Discussionmentioning

confidence: 99%

“…Similar to the genus Terrisporobacter , the biotechnological applications of bacteria from the genus Acetonaerobium were restricted to the degradation of protein-rich wastes to H 2 . A. sticklandii was shown to ferment amino acids to acetate, butyrate, acetone, butanol and ethanol as products ( Sangavai and Chellapandi, 2019 ). Similar to Terrisporobacter , Acetoanaerobium noterae is a potential electrotroph as it was enriched at the cathode of MES cells ( Wang et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of novel acetogenic strains of the genera Terrisporobacter and Acetoanaerobium

Böer,

Schüler,

Lüschen

et al. 2024

Front. Microbiol.

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Due to their metabolic versatility in substrate utilization, acetogenic bacteria represent industrially significant production platforms for biotechnological applications such as syngas fermentation, microbial electrosynthesis or transformation of one-carbon substrates. However, acetogenic strains from the genera Terrisporobacter and Acetoanaerobium remained poorly investigated for biotechnological applications. We report the isolation and characterization of four acetogenic Terrisporobacter strains and one Acetoanaerobium strain. All Terrisporobacter isolates showed a characteristic growth pattern under a H2 + CO2 atmosphere. An initial heterotrophic growth phase was followed by a stationary growth phase, where continuous acetate production was indicative of H2-dependent acetogenesis. One of the novel Terrisporobacter isolates obtained from compost (strain COMT) additionally produced ethanol besides acetate in the stationary growth phase in H2-supplemented cultures. Genomic and physiological characterizations showed that strain COMT represented a novel Terrisporobacter species and the name Terrisporobacter vanillatitrophus is proposed (=DSM 116160T = CCOS 2104T). Phylogenomic analysis of the novel isolates and reference strains implied the reclassification of the T. petrolearius/T. hibernicus phylogenomic cluster to the species T. petrolearius and of the A. noterae/A. sticklandii phylogenomic cluster to the species A. sticklandii. Furthermore, we provide first insights into active prophages of acetogens from the genera Terrisporobacter and Acetoanaerobium.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of novel acetogenic strains of the genera Terrisporobacter and Acetoanaerobium

Böer,

Schüler,

Lüschen

et al. 2024

Front. Microbiol.

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“…Interestingly, its metabolic intermediates from gelatin catabolism mediated not only the synthesis of biofuel components but also some therapeutic compounds. The biofuel‐producing efficiency of this organism has been evaluated from amino acids and gelatin, but its growth patterns for protein catabolism are not yet revealed [24]. Ossein (bone) factory waste has been exploited as a potential protein‐based biomass for biofuel production in gelatin processing industries.…”

Section: Introductionmentioning

confidence: 99%

Growth‐associated catabolic potential of Acetoanaerobium sticklandii DSM 519 on gelatin and amino acids

Sangavai

Chellapandi

2020

J Basic Microbiol

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Acetoanaerobium sticklandii DSM 519 is a hyperammonia‐producing anaerobe that catabolizes proteins and amino acids into organic solvents and volatile acids via the Stickland reactions. However, the specific growth rate and metabolic capability of this organism on proteins and amino acids are not yet known. Therefore, the present study was intended to evaluate its specific growth rate and metabolic potential on gelatin and amino acids in the experimental media. We carried out metabolic assay experiments to calculate its ability to utilize pure gelatin, single amino acids, and amino acid pairs at different growth phases. The results of this study show that complete assimilation of gelatin was achieved by its log‐phase culture. The subsequent fermentation of amino acids was much faster than gelatin hydrolysis. The rate of gelatin degradation was associated with the growth and catabolic rates of this organism. Many amino acids were not assimilated completely for its growth and energy conservation. A log‐phase culture of this organism preferably utilized l‐cysteine, l‐arginine, and l‐serine, and released more fraction of ammonia. As shown by our analysis, the catabolic rates of these amino acids were determined by the rates of respective enzymes involved in amino acid catabolic pathways and feedback repression of ammonia. The growth kinetic data indicated that at the initial growth stage, a metabolic shift in its solventogenesis and acidogenesis phases was associated with catabolism of certain amino acids. Thus, the results of this study provide a new insight to exploit its log‐phase culture as a starter for the production of biofuel components from gelatin processing industries.

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Deciphering metabolic regulatory mechanisms in vital anaerobes for enhanced biogas production from protein-rich waste

Thamanna,

Chellapandi

2024

Syst Microbiol and Biomanuf

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A metabolic study to decipher amino acid catabolism-directed biofuel synthesis in Acetoanaerobium sticklandii DSM 519

Cited by 6 publications

References 58 publications

Isolation and characterization of novel acetogenic strains of the genera Terrisporobacter and Acetoanaerobium

Isolation and characterization of novel acetogenic strains of the genera Terrisporobacter and Acetoanaerobium

Growth‐associated catabolic potential of Acetoanaerobium sticklandii DSM 519 on gelatin and amino acids

Deciphering metabolic regulatory mechanisms in vital anaerobes for enhanced biogas production from protein-rich waste

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