A metal-organic framework based PCR-free biosensor for the detection of gastric cancer associated microRNAs

Qiu, Guang; Lu, Wanzhen; Hu, Peipei; Jiang, Zhi‐Hong; Bai, Liping; Wang, Tao-Rui; Li, Minmin; Chen, Jinxiang

doi:10.1016/j.jinorgbio.2017.08.036

Cited by 29 publications

(7 citation statements)

References 48 publications

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“…Such assumption is further evidenced by the alteration of the fluorescence anisotropy (FA) of the P-DNA and ds-DNA@Hg 2+ (hybrid duplex) prior and after the introduction of MOF 1 , respectively. It is established that when a fluorophore is bound to a molecule, e.g., FAM to ss-DNA, to give P-DNA in the present case, the motion-related factors increase when the P-DNA was adsorbed by MOF 1 due to its large molecular size . Thus, FA functions as a means to estimate if P-DNA or ds-DNA@Hg 2+ is associated with the surface of MOF 1 .…”

Section: Resultsmentioning

confidence: 74%

“…We conducted all the detection process in Hepes buffer (pH 6.8–8.2) as it showed higher detection sensitivity and selectivity than in Tris-HCl (pH 7.1–9.0) and MOPS (MOPS = 3-( N -morpholino)propanesulfonic acid, pH 6.5–7.9). The construction of P-DNA@ 1 hybrid system (Hg 2+ sensor) is based on the affinity between MOF 1 and P-DNA, which has been demonstrated in our previous studies. − In order to verify the detection sensitivity of Hg 2+ and I – , we conducted the following experiments. In the first step, we established the optimal Hg 2+ sensor by mixing MOF 1 and T-rich P-DNA (50 nM) in Hepes buffer (pH 7.4, 20 mM).…”

Section: Resultsmentioning

confidence: 99%

“…This unique ligand further lined up with positively charged Cu 2+ to yield a 2D sheet structure with high surface exposure. MOF 1 is thus able to associate with the P-DNA through a variety of intermolecular forces, such as electrostatic, π-stacking, and/or hydrogen-bonding, to give a P-DNA@ 1 hybrid with quenched FAM emission. − Second, in the subsequent emission recovery step, MOF 1 plays a vital role in distinguishing single-stranded P-DNA (ss-P-DNA) from the ds-DNA@Hg 2+ duplex . Due to the absence of unpaired bases, the relatively rigid structures, and the large cross-sectional areas, the formed ds-DNA@Hg 2+ duplex fails to effectively interact with MOF 1 .…”

Section: Resultsmentioning

confidence: 99%

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Successive and Specific Detection of Hg²⁺ and I^– by a DNA@MOF Biosensor: Experimental and Simulation Studies

Liu

et al. 2018

Inorg. Chem.

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A 2D metal-organic framework (MOF) of {[Cu(Dcbb)(Bpe)]·Cl} (1, HDcbbBr = 1-(3,5-dicarboxybenzyl)-4,4'-bipyridinium bromide, Bpe = trans-1,2-bis(4-pyridyl)ethylene)) has been prepared. MOF 1 associates with the thymine-rich (T-rich), single-stranded probe DNA (ss-DNA, denoted as P-DNA) labeled with fluorophore FAM (FAM = carboxyfluorescein) and quenches the FAM emission to give a nonemissive P-DNA@1 hybrid (off state). The P-DNA in the hybrid subsequently captures the Hg to give a rigid double-stranded DNA featuring T-Hg-T motif (ds-DNA@Hg) and detach from MOF 1, triggering the recovery of the FAM fluorescence (on state). Upon subsequent addition of I, Hg was further sequestrated from the ds-DNA@Hg duplex, driven by the stronger Hg-I coordination. The released P-DNA is resorbed by MOF 1 to regain the initial P-DNA@1 hybrid (off state). The P-DNA@1 sensor thus detects Hg and I sequentially via a fluorescence "off-on-off" mechanism. The sensor is highly selective and sensitive, yielding detection limits of 3.2 and 3.3 nM, respectively. The detection process was conformed by circular dichroism (CD) and the detection mechanism was verified by fluorescence anisotropy, binding constant, and simulation of the binding free energy at each stage.

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Section: Resultsmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Successive and Specific Detection of Hg²⁺ and I^– by a DNA@MOF Biosensor: Experimental and Simulation Studies

Liu

et al. 2018

Inorg. Chem.

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“…Their team provides a very effective strategy for miRNA monitoring. Qiu bound five DNA probes to MOF1, which specifically recognize five microRNAs in gastric cancer cells [83]. After the DNA molecule is complementary to the target microRNA, the fluorescent molecule emits a signal, which provides us with immediate diagnostic information.…”

Section: Mof-based Nanotherapeutics For Gene Deliverymentioning

confidence: 99%

Metal Organic Frameworks as Drug Targeting Delivery Vehicles in the Treatment of Cancer

et al. 2020

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In recent years, metal organic frameworks (MOFs) have been widely developed as vehicles for the effective delivery of drugs to tumor tissues. Due to the high loading capacity and excellent biocompatibility of MOFs, they provide an unprecedented opportunity for the treatment of cancer. However, drugs which are commonly used to treat cancer often cause side effects in normal tissue accumulation. Therefore, the strategy of drug targeting delivery based on MOFs has excellent research significance. Here, we introduce several intelligent targeted drug delivery systems based on MOFs and their characteristics as drug-loading systems, and the challenges of MOFs are discussed. This article covers the following types of MOFs: Isoreticular Metal Organic Frameworks (IRMOFs), Materials of Institute Lavoisier (MILs), Zeolitic Imidazolate Frameworks (ZIFs), University of Oslo (UiOs), and MOFs-based core-shell structures. Generally, MOFs can be reasonably controlled at the nanometer size to effectively achieve passive targeting. In addition, different ligands can be modified on MOFs for active or physicochemical targeting. On the one hand, the targeting strategy can improve the concentration of the drugs at the tumor site to improve the efficacy, on the other hand, it can avoid the release of the drugs in normal tissues to improve safety. Despite the challenges of clinical application of MOFs, MOFs have a number of advantages as a kind of smart delivery vehicle, which offer possibilities for clinical applications.

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“…The observed cross-reactivity was negligible when using other fluoresce-labeled DNA sequences, but neither reproducibility between different batches nor recyclability studies were performed in this work. A similar biosensor scheme was proposed by Qiu and colleagues for the detection of five different gastric cancer associated micro RNAs (miRNAs) [62]. For this purpose, a 1D Cu-MOF was prepared using Cu(NO 3 ) 2 and a sodium salt of 1-(3,5-dicarboxybenzyl)-4,4′-bipyridinium bromide (H 2 dcbbBr) in water following a solvothermal synthesis.…”

Section: Mofs For Biosensingmentioning

confidence: 99%

Metal-Organic Frameworks for the Development of Biosensors: A Current Overview

Carrasco

2018

Biosensors

126

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This review focuses on the fabrication of biosensors using metal-organic frameworks (MOFs) as recognition and/or transducer elements. A brief introduction discussing the importance of the development of new biosensor schemes is presented, describing these coordination polymers, their properties, applications, and the main advantages and drawbacks for the final goal. The increasing number of publications regarding the characteristics of these materials and the new micro- and nanofabrication techniques allowing the preparation of more accurate, robust, and sensitive biosensors are also discussed. This work aims to offer a new perspective from the point of view of materials science compared to other reviews focusing on the transduction mechanism or the nature of the analyte. A few examples are discussed depending on the starting materials, the integration of the MOF as a part of the biosensor and, in a deep detail, the fabrication procedure.

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A metal-organic framework based PCR-free biosensor for the detection of gastric cancer associated microRNAs

Cited by 29 publications

References 48 publications

Successive and Specific Detection of Hg²⁺ and I^– by a DNA@MOF Biosensor: Experimental and Simulation Studies

Successive and Specific Detection of Hg²⁺ and I^– by a DNA@MOF Biosensor: Experimental and Simulation Studies

Metal Organic Frameworks as Drug Targeting Delivery Vehicles in the Treatment of Cancer

Metal-Organic Frameworks for the Development of Biosensors: A Current Overview

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A metal-organic framework based PCR-free biosensor for the detection of gastric cancer associated microRNAs

Cited by 29 publications

References 48 publications

Successive and Specific Detection of Hg2+ and I– by a DNA@MOF Biosensor: Experimental and Simulation Studies

Successive and Specific Detection of Hg2+ and I– by a DNA@MOF Biosensor: Experimental and Simulation Studies

Metal Organic Frameworks as Drug Targeting Delivery Vehicles in the Treatment of Cancer

Metal-Organic Frameworks for the Development of Biosensors: A Current Overview

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Successive and Specific Detection of Hg²⁺ and I^– by a DNA@MOF Biosensor: Experimental and Simulation Studies

Successive and Specific Detection of Hg²⁺ and I^– by a DNA@MOF Biosensor: Experimental and Simulation Studies