A method for assessing mitochondrial physiology using mechanically permeabilized flight muscle of Aedes aegypti mosquitoes

Gaviraghi, A.; Oliveira, Marcus F.

doi:10.1016/j.ab.2019.04.005

Cited by 16 publications

(16 citation statements)

References 52 publications

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“…ATP production derived through oxidative phosphorylation (OXPHOS) provides the energy needed to support flight activity. To assess the potential impact of the diet and exercise interventions on the OXPHOS capacity of flies, mechanically permeabilized flight muscles were prepared as previously described 29 and used to measure the mitochondrial respiratory capacity in single fruit flies by high‐resolution respirometry. To validate this model, we performed experiments to evaluate flight muscle permeabilization and the integrity of mitochondrial membranes to different substrates.…”

Section: Resultsmentioning

confidence: 99%

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Preference and detrimental effects of high fat, sugar, and salt diet in wild‐caught Drosophila simulans are reversed by flight exercise

et al. 2020

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Section: Resultsmentioning

confidence: 99%

“…To assess the oxidative phosphorylation capacity of flies, mechanically permeabilized flight muscles were prepared as previously described 29 . Briefly, flies were immobilized by cooling on cold block 0ºC and dissected by removing sequentially head, wings, legs, and abdomen.…”

Section: Methodsmentioning

confidence: 99%

Preference and detrimental effects of high fat, sugar, and salt diet in wild‐caught Drosophila simulans are reversed by flight exercise

et al. 2020

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“…For example, while blood-sucking insects like Anopheles , Aedes and tse-tse fly use proline, some other insects, such as locust and, cockroaches use trehalose instead [ 151 – 154 ]. In Aedes , a combination of proline and pyruvate (pyruvate can be obtained from trehalose metabolism since trehalose is the major sugar in insect hemolymph) provided the highest energy needed for flight [ 155 ]. Similarly, Anopheles can use proline and pyruvate to fuel flight [ 154 ].…”

Section: Metabolic Proteins Blood Digestion Immune Response and mentioning

confidence: 99%

Anopheles metabolic proteins in malaria transmission, prevention and control: a review

et al. 2020

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The increasing resistance to currently available insecticides in the malaria vector, Anopheles mosquitoes, hampers their use as an effective vector control strategy for the prevention of malaria transmission. Therefore, there is need for new insecticides and/or alternative vector control strategies, the development of which relies on the identification of possible targets in Anopheles. Some known and promising targets for the prevention or control of malaria transmission exist among Anopheles metabolic proteins. This review aims to elucidate the current and potential contribution of Anopheles metabolic proteins to malaria transmission and control. Highlighted are the roles of metabolic proteins as insecticide targets, in blood digestion and immune response as well as their contribution to insecticide resistance and Plasmodium parasite development. Furthermore, strategies by which these metabolic proteins can be utilized for vector control are described. Inhibitors of Anopheles metabolic proteins that are designed based on target specificity can yield insecticides with no significant toxicity to non-target species. These metabolic modulators combined with each other or with synergists, sterilants, and transmission-blocking agents in a single product, can yield potent malaria intervention strategies. These combinations can provide multiple means of controlling the vector. Also, they can help to slow down the development of insecticide resistance. Moreover, some metabolic proteins can be modulated for mosquito population replacement or suppression strategies, which will significantly help to curb malaria transmission.

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“…The respiratory activity of mechanically permeabilized flight muscle and isolated mitochondria from A. aegypti was carried out in a two-channel titration injection respirometer (Oxygraph-2k, Oroboros Instruments, Innsbruck, Austria) at 27.5 °C using methods previously established by our group [11,74]. Two distinct substrate combinations were used in our experiments as following: pyruvate + proline (Pyr+Pro) or sn-glycerol 3-phosphate (G3P) in presence and absence of ADP.…”

Section: Respirometry Analysesmentioning

confidence: 99%

Mitochondrial glycerol phosphate oxidation is modulated by adenylates through allosteric regulation of cytochromecoxidase activity in mosquito flight muscle

Gaviraghi

Soares

Mignaco

et al. 2019

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The huge energy demand posed by insect flight activity is met by an efficient oxidative phosphorylation process that takes place within flight muscle mitochondria. In the major arbovirus vector Aedes aegypti, mitochondrial oxidation of pyruvate, proline and glycerol 3 phosphate (G3P) represent the major energy sources of ATP to sustain flight muscle energy demand. Although adenylates exert critical regulatory effects on several mitochondrial enzyme activities, the potential consequences of altered adenylate levels to G3P oxidation remains to be determined. Here, we report that mitochondrial G3P oxidation is controlled by adenylates through allosteric regulation of cytochrome c oxidase (COX) activity in A. aegypti flight muscle. We observed that ADP significantly activated respiratory rates linked to G3P oxidation, in a protonmotive force-independent manner. Kinetic analyses revealed that ADP activates respiration through a slightly cooperative mechanism. Despite adenylates caused no effects on G3P-cytochrome c oxidoreductase activity, COX activity was allosterically activated by ADP. Conversely, ATP exerted powerful inhibitory effects on respiratory rates linked to G3P oxidation and on COX activity. We also observed that high energy phosphate recycling mechanisms did not contribute to the regulatory effects of adenylates on COX activity or G3P oxidation. We conclude that mitochondrial G3P oxidation by A. aegypti flight muscle is regulated by adenylates essentially through the allosteric modulation of COX activity, underscoring the bioenergetic relevance of this novel mechanism and the potential consequences for mosquito dispersal.

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A method for assessing mitochondrial physiology using mechanically permeabilized flight muscle of Aedes aegypti mosquitoes

Cited by 16 publications

References 52 publications

Preference and detrimental effects of high fat, sugar, and salt diet in wild‐caught Drosophila simulans are reversed by flight exercise

Preference and detrimental effects of high fat, sugar, and salt diet in wild‐caught Drosophila simulans are reversed by flight exercise

Anopheles metabolic proteins in malaria transmission, prevention and control: a review

Mitochondrial glycerol phosphate oxidation is modulated by adenylates through allosteric regulation of cytochromecoxidase activity in mosquito flight muscle

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