A Method for Collecting Atmospheric Microbial Samples From Set Altitudes for Use With Next-Generation Sequencing Techniques to Characterize Communities

Abstract

Dispersal of airborne microorganisms is an important ecological process, resulting in the distribution of bacteria to all habitats on Earth. Investigation of this process is limited by the ability to collect uncontaminated high-altitude microbial samples for use with next-generation sequencing approaches. Here, we describe the design of a Remote Airborne Microbial Passive sampling system. Troubleshooting experiments demonstrate that the samplers collect adequate DNA for bacterial 16S rRNA (ribosomal RNA) amplicon-based Mi-Seq sequencing at 2 and 150 m from the ground. When samplers are closed, they retain only a low number of sequences, and may be used as a negative control. We also demonstrate that the optimal amount of collection dishes to include in the sampler is 8, and that freezing collection dishes at −80°C is an alternative to immediate DNA extraction. Samplers may be used to address a variety of ecological and human health-related questions.

Investigation of Sources, Diversity, and Variability of Bacterial Aerosols in Athens, Greece: A Pilot Study

“…Therefore, speci c measures and precautions in all steps of sampling and analysis have to be taken. This issue is common across the entire microbial ecology eld and has to be considered in studies where DNA yields are expected to be low (Spring et al 2018).…”

Composição da comunidade bacteriana aérea de Santarém, Pará: influência de aves

“…Entretanto, os resultados por ponto de amostragem mostraram que o ponto P3 teve maior número de bactérias heterotróficas na primeira e terceira coletam, o ponto P2, na segunda, e o ponto P1 na quarta coleta (Figura 2). Microorganismos de várias fontes, contribuem para uma carga microbiana uniforme e diluída em uma determinada parcela de ar, e podem estar relacionados ao transporte aéreo do solo e outros reservatórios como função das condições ambientais (SPRING et al, 2018). Contudo, ao encontrar uma fonte de partículas transportadas pelo ar, é esperada que a concentração local de micro-organismos seja maior e diferente do conjunto de micro-organismos que se originaram em locais mais distantes (LYMPEROPOULOU et al, 2016).…”

“…O ar e toda diversidade de micro-organismos contida nele se conecta com os demais ecossistemas terrestres e aquáticos. Uma vez depositados na superfície, os micro-organismos são responsáveis por etapas importantes em todos os principais ciclos de nutrientes, mas muito pouco é conhecido sobre os mecanismos que controlam os efeitos microbianos atmosféricos dispersos para os ecossistemas (SPRING et al, 2018). O campo da aerobiologia é relativamente novo, carente de estudos, especialmente na Amazônia (WOMACK et al, 2010).…”

Study of metrological characteristics of low-cost digital temperature sensors for greenhouse conditions

“…In other scientific sources [10,21] it is suggested to use SHT11 sensor as a temperature detecting element when developing such systems. Similar studies on the development and simulation of systems for monitoring the physical media temperature are presented by using SHT21 [17], BMP180 [13], BME280 [2] and DHT22 sensors [8,16]. The articles [7,19] present the main approaches to the assessment and analysis of the metrological and functional characteristics of sensors, which are used in constructing modern measuring systems for nondestructive testing of parameters of physical and chemical processes.…”