A Method for Collecting Atmospheric Microbial Samples From Set Altitudes for Use With Next-Generation Sequencing Techniques to Characterize Communities

Spring, Allison M; Docherty, Kathryn M.; Domingue, K. D.; Kerber, Thomas V.; Mooney, Margaret; Lemmer, Kristina

doi:10.25384/sage.c.4183172.v1

2018

DOI: 10.25384/sage.c.4183172.v1

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A Method for Collecting Atmospheric Microbial Samples From Set Altitudes for Use With Next-Generation Sequencing Techniques to Characterize Communities

Allison M Spring

Kathryn M. Docherty

K. D. Domingue

et al.

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2020

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Cited by 1 publication

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Earth's Stratosphere and Microbial Life

DasSarma¹,

Antunes²,

Simões³

et al. 2020

Current Issues in Molecular Biology

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The Earth's atmosphere is an extremely large and sparse environment which is quite challenging for the survival of microorganisms. We have long wondered about the limits to life in the atmosphere, starting with Leeuwenhoek's observation of "animalcules" collected from the air. In the past century, significant progress has been made to capture and identify biological material from varying elevations, from a few meters above ground level, to the clouds near mountaintops, and the jet streams, the ozone layer, and even higher up in the stratosphere. Collection and detection techniques have been developed and advanced in order to assess the potential diversity of life from very high altitudes. Studies of microbial life in the stratosphere with its multiple stressors (cold, dry, irradiated, with low pressure and limited nutrients), have recently garnered considerable attention. Here, we review studies of Earth's atmosphere, with emphasis on the stratosphere, addressing implications for astrobiology, the dispersal of microbes around our planet, planetary protection, and climate change.

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Earth's Stratosphere and Microbial Life

DasSarma¹,

Antunes²,

Simões³

et al. 2020

Current Issues in Molecular Biology

View full text Add to dashboard Cite

show abstract

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A Method for Collecting Atmospheric Microbial Samples From Set Altitudes for Use With Next-Generation Sequencing Techniques to Characterize Communities

Cited by 1 publication

References 41 publications

Earth's Stratosphere and Microbial Life

Earth's Stratosphere and Microbial Life

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