As the density of the high-speed railway network continues to increase, the problem of electromagnetic interference on adjacent lines has become increasingly prominent. This paper focuses on the electromagnetic interference of adjacent lines caused by rail and line in the signal transmission process of the high-speed rail track circuit. Firstly, complete the establishment of the four-terminal network model of the ZPW-2000A track circuit system and the cab signal entry current crosstalk model, calculation of interference voltage under different parallel length of signal frequency. Then the interference factors and coupling mechanism of adjacent lines are analysed to realize calculation of interference amount. Finally, according to the sensitivity index of the cab signal, the maximum parallel length of adjacent sections is given respect, and the interference protection suggestions of adjacent lines are put forward. The research work of this paper provides a theoretical basis for suppressing the interference of adjacent lines and guarantees the safe and efficient operation of high-speed trains.