A method for measurement of liver iron fractions in needle biopsy specimens and some results in acute liver disease

Zuyderhoudt, F. M. J.; Hengeveld, Paul J.; Gool, J. van; Jörning, G.G.A.

doi:10.1016/0009-8981(78)90387-x

Cited by 50 publications

(35 citation statements)

References 13 publications

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“…With an average increase in [Mb] in this muscle mass from 25 to ~35mgg −1 within the fasting period (Fig.4B), about 88g of Mb may have been synthesized, which would require mobilization of 280mg of iron (given the need for one Fe for each Mb molecule and a molecular weight ratio for Fe/Mb of 55.84/17,380). The estimated decrease in liver iron content in the same period was about 182mg (Table1), which is fairly close to the estimated iron required for Mb synthesis, particularly when considering the remarkable variation in the liver iron concentration in seals, as in other species (Zuyderhoudt et al, 1978;Faa et al, 1994;Bonkovsky et al, 1999). In estimating liver iron content in young pups (newborn/weaned; Table1), we have pooled the data on liver mass and liver iron concentration for all pups aged 1-10days, as there was no significant difference in the liver data within this age group.…”

Section: Discussionsupporting

confidence: 75%

Rapid postnatal development of myoglobin from large liver iron stores in hooded seals

Geiseler

Blix

Burns

et al. 2013

Journal of Experimental Biology

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SUMMARYHooded seals (Cystophora cristata) rely on large stores of oxygen, either bound to hemoglobin or myoglobin (Mb), to support prolonged diving activity. Pups are born with fully developed hemoglobin stores, but their Mb levels are only 25-30% of adult levels. We measured changes in muscle [Mb] from birth to 1year of age in two groups of captive hooded seal pups, one being maintained in a seawater pool and one on land during the first 2months. All pups fasted during the first month, but were fed from then on. The [Mb] of the swimming muscle musculus longissimus dorsi (LD) doubled during the month of fasting in the pool group. These animals had significantly higher levels and a more rapid rise in LD [Mb] than those kept on land. The [Mb] of the shoulder muscle, m. supraspinatus, which is less active in both swimming and hauled-out animals, was consistently lower than in the LD and did not differ between groups. This suggests that a major part of the postnatal rise in LD [Mb] is triggered by (swimming) activity, and this coincides with the previously reported rapid early development of diving capacity in wild hooded seal pups. Liver iron concentration, as determined from another 25 hooded seals of various ages, was almost 10 times higher in young pups (1-34days) than in yearling animals and adults, and liver iron content of pups dropped during the first month, implying that liver iron stores support the rapid initial rise in [Mb].

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Section: Discussionsupporting

confidence: 75%

Rapid postnatal development of myoglobin from large liver iron stores in hooded seals

Geiseler

Blix

Burns

et al. 2013

Journal of Experimental Biology

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“…4 g/l protein) by heat treatment (70-75°C, 5min) and ce trifugation was performed s described by Zuyderhoudt et al (4). Ferritin iron was measured in the superaatant, diluted (1+2) with 200 mmol/1 ammonium acetate^ 30 mmol/1 nitric acid.…”

Section: Ferritin Determinationmentioning

confidence: 99%

Determination of Tissue Iron and Ferritin in Liver Pathology. Comparison of Histochemical and Biochemical Results

Deursen

Dieijen-Visser²,

Koudstaal³

et al. 1989

Clinical Chemistry and Laboratory Medicine

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Summary:Results are presented from the determination of tissue iron and ferritin in 15 pathological livers (6 with steatosis, 8 with fibrosis/cirrhosis and l with haemochromatosis). The histological assessment according to the Rowe System, after Perls* staining, was compared with the measurement of the iron content in liver homogenate by flameless atomic absoφtion spectrophotometry.The results of the flameless atomic absorption spectrophotometry were less accurate than in normal livers, but the method can still be considered reliable with satisfactory precision.As in normal livers, the r nge of chemically determined values in the histological staining grades was considerable and there was quite an overlap between consecutive grades. The chemical determination of liver iron content is to be preferred.

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“…Iron was estimated in biopsy material by a micromethod using dry ashing and bathophenanthroline reagents [16,20], The assays were performed in a microtiter plate, in a final volume of 250 pi of reaction mixture. The intensity of color was measured in a microtiter plate reader (Toyo, Japan) at 540 nm.…”

Section: Estimation O F Iron In Liver Biopsy Samplesmentioning

confidence: 99%

Induction of Dietary Iron Deficiency in Rhesus Monkeys: Sequential Changes in Serum Ferritin and Other Biochemical Indicators of Iron Status

et al. 1994

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The usefulness of serum ferritin as a measure of subclinical stages of iron deficiency has been tested in monkeys by inducing a mild iron deficiency dietarily over a period of 12 months. Various biochemical indicators of iron status were measured periodically along with analysis of liver iron and iron staining of bone marrow samples obtained by biopsy, at the end of the experiment. A mild form of iron deficiency was confirmed by bone marrow staining for iron. Of all the biochemical indicators tested, significant decreases were seen in hemoglobin and hematocrit at the 11th and 10th months, respectively. These changes were consistent with the changes later found in bone marrow grading for iron. Serum ferritin concentration and liver iron concentration did not show any significant difference between the controls and iron-deficient monkeys. Thus, these results do not support the existence of a latent stage of iron deficiency. In the mild form of iron deficiency, the functional compartment, represented by bone marrow iron and hemoglobin, is sensitive to depletion even when there were no changes in storage compartment represented by liver iron and serum ferritin.

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A method for measurement of liver iron fractions in needle biopsy specimens and some results in acute liver disease

Cited by 50 publications

References 13 publications

Rapid postnatal development of myoglobin from large liver iron stores in hooded seals

Rapid postnatal development of myoglobin from large liver iron stores in hooded seals

Determination of Tissue Iron and Ferritin in Liver Pathology. Comparison of Histochemical and Biochemical Results

Induction of Dietary Iron Deficiency in Rhesus Monkeys: Sequential Changes in Serum Ferritin and Other Biochemical Indicators of Iron Status

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