A Method for the Examination of the Substrate Mycelium of Actinomycetes by Scanning Electron Microscopy

Abstract

The polyol gel Lutrol FC127 was used to solidify culture media. This gel liquefies as the temperature drops below a critical value for the concentration used. This property was used to recover whole colonies of Thermoactinomyces sp. for examination of the substrate mycelium by scanning electron microscopy.