A method to determine shear adhesive strength of fibrin sealants

Abstract

The adhesive strength of fibrin sealants has not been rigorously evaluated to date. The adhesive strength of six different concentrations of cryoprecipitated fibrinogen as well as the commercially available fibrin tissue adhesive Tissucol was tested under controlled conditions utilizing split-thickness skin grafts as the test adherand. This test configuration permitted the modeling of bonding strength for attachment of skin grafts as well as incorporate established engineering test standards for adhesives. An increase in fibrin concentration corresponded with an increase in shear adhesive strength. No significant increases in adhesive strength were attained after 5 min of bonding for all tested concentrations, except for the commercial adhesive, which attained the adhesive strength of an equivalent concentration of cryoprecipitated adhesive after 90 min. The adhesive strength, however, was an order of magnitude less than reported values of the tensile strength of fibrin material for similar concentrations. Therefore, it is important that the surgeon use a sufficiently high fibrinogen concentration for the specific clinical indication. The method of fibrin sealant preparation and/or the compounding adjuncts appear to have an effect on the development of adhesive strength.

Failure characteristics of multiple‐component fibrin‐based adhesives

“…Gelation time prior to pressurization, which has been shown to influence adhesive strength are not reported in these studies. 12 There is little difference in the fibrin concentrations reported for commercial sealants (i.e., 60-70 mg/mL fibrinogen concentration). The Pb values reported in this study are relatively high in comparison to the published reports because of the high affinity of fibrin to dermis.…”

“…The mechanical analysis of fibrin-based biomaterials has hitherto been focused on bulk uniaxial tensile, 1,2 dynamic shear properties, [3][4][5][6][7] burst pressure, [8][9][10] and tensile and shear adhesive strength. [11][12][13] These investigations describe mechanical strength and modulus as proportional to fibrinogen concentration, and that bonding strength varies with different adherends. Furthermore, work of adhesion has been reported for fibrin-based and albumin-polymer sealants, 14,15 and adhesive energy has been reported for marine-origin substances.…”

Development of a biodegradable tissue adhesive based on functionalized 1,2‐ethylene glycol bis(dilactic acid). II.

“…7 By contrast, naturally based fibrin adhesives, while less reactive and more biocompatible, have insufficient adhesive strength. 3,4,[9][10][11] To overcome these limitations, various adhesives based on epoxides, 12 polymethacrylates, 12 gelatin-resorcinol-aldehydes, 3,13 protein-aldehydes, 14 mussel adhesive proteins 3,15,16 as well as oligoand polylactones 17,18 were developed. Additionally, isocyanate-based adhesives are under consideration.…”

Thermal gelation and tissue adhesion of biomimetic hydrogels

“…Several labs have reported on efforts to lower cytotoxicity of these systems [5][6][7]. The fibrin glues, on the other hand, are generally considered highly biocompatible and adhesive to tissue surfaces but have very low cohesive strength [8,9]. The use of blood components in the production of fibrin glues remains a concern as it introduces a risk of disease transmission.…”