A methodological study of adhesion dynamics in a batch culture of the marine microalga Nannochloropsis gaditana

Zeriouh, Ouassim; Reinoso-Moreno, J.V.; López-Rosales, L.; Sierra‐Martin, Benjamin; Cerón-García, M.C.; Sánchez-Mirón, A.; Fernández-Barbero, A.; Camacho, F. Garcı́a; Molina-Grima, E.

doi:10.1016/j.algal.2017.02.008

Cited by 21 publications

(16 citation statements)

References 43 publications

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“…The culture medium consisted of natural seawater supplemented with macro and micronutrients. The exact composition of the culture medium is described elsewhere (Zeriouh, Reinoso‐Moreno, López‐Rosales, Sierra‐Martín, et al, ). A glass bench‐scale flat‐panel photobioreactor (FP‐PBR) was used for the indoor experiments.…”

Section: Methodsmentioning

confidence: 99%

“…The cell status of the cultures was checked daily by measuring the maximum photochemical yield of the photosystem II (PSII), F v / F m , ratio with a fluorometer (Mini‐PAM‐2500, Heinz Walz, Effeltrich, Germany). The nitrate and phosphate concentrations dissolved in the supernatant and the biomass concentration in the cultures were determined spectrophotometrically as described earlier (Zeriouh et al, ; Zeriouh, Reinoso‐Moreno, López‐Rosales, Sierra‐Martín, et al, ). Additionally, flow cytometry was used to quantify the cultures’ cell density (cells per ml).…”

Section: Methodsmentioning

confidence: 99%

“…The culture flow rate that circulated continuously through the MRD was 25 L/h. In continuous culture mode, the MRD was disconnected from the FP‐PBR every 2 days, washed gently with sterile seawater and then the density of the adhered cells per unit area ( B ) on each of the coupons (cells cm −2 ) was quantified, as previously described (Zeriouh et al, ; Zeriouh, Reinoso‐Moreno, López‐Rosales, Sierra‐Martín, et al, ). These were then placed again in the MRD and reconnected to the FP‐PBR for the next measurements.…”

Section: Methodsmentioning

confidence: 99%

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New insights into developing antibiofouling surfaces for industrial photobioreactors

Zeriouh

Marco‐Rocamora

Reinoso-Moreno

et al. 2019

Biotech & Bioengineering

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The biofouling formation of the marine microalga Nannochloropsis gaditana on nontoxic surfaces was quantified on rigid materials, both coated (with fouling release coatings and nanoparticle coatings) and noncoated, to cover a wide range of surface properties from strongly hydrophobic to markedly hydrophilic under conditions similar to those prevailing in outdoor massive cultures of marine microalgae. The effect of seawater on surfaces that presented the best antibiofouling properties was also evaluated. The adhesion intensity on the different surfaces was compared with the predictions of the biocompatibility theories developed by Baier and Vogler using water adhesion tension (τ 0 ) as the quantitative parameter of surface wettability. For the most hydrophobic surfaces, τ 0 ≤ 0, the microalgae adhesion density increased linearly with τ 0 , following the Baier's theory trend. However, for the rest of the surfaces, τ 0 ≥ 0, a tendency toward minimum adhesion was observed for amphiphilic surfaces with a τ 0 = 36 mJ/m 2 , a value close to that which minimizes cell adhesion according to Vogler's theory. The understanding and combination of the two biocompatibility theories could help to design universal antibiofouling surfaces that minimize the van der Waals forces and prevent foulant adsorption by using a thin layer of hydration. K E Y W O R D Santibiofouling, biocompability theory, fouling release coatings, microalga, photobioreactor

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

New insights into developing antibiofouling surfaces for industrial photobioreactors

Zeriouh

Marco‐Rocamora

Reinoso-Moreno

et al. 2019

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…Regarding the attachment of the cells to the coupons, once the cultivation experiments were finished, the coupons were carefully detached from the vessels and transferred into a flask filled with plenty of sterile Mediterranean seawater to remove non-adherent cells and debris. The microalgae adhesion intensity on the coupons was evaluated using a procedure based on the Chla fluorescence of the attached algae cells, as previously described (Zeriouh et al, 2017a).…”

Section: Monitoring Microalgal Growth and Attachmentmentioning

confidence: 99%

“…This work aims to evaluate the biofouling susceptibility of different materials and coatings immersed in cultures of the marine microalgaNannochloropsis gaditana , a species used as model in studies on biofouling formation in photobioreactors (Zeriouh et al, 2019a;Zeriouh et al, 2019b;Zeriouh et al, 2017a). As a first step, the surfaces were tested in short-term batch adhesion experiments with the model protein BSA (Wang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Long-term biofouling formation mediated by extracellular proteins in Nannochloropsis gaditana microalga cultures at different medium N/P ratios

Soriano-Jerez¹,

López-Rosales²,

Cerón-García³

et al. 2020

Preprint

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Biofouling represents an important limitation in photobioreactor cultures. The biofouling propensity of different materials (polystyrene, borosilicate glass, polymethyl methacrylate and polyethylene terephthalate glycol-modified) and coatings (two spray-applied and nanoparticle-based superhydrophobic coatings and a hydrogel-based fouling release coating) was evaluated by means of a short-term protein test, using bovine serum albumin (BSA) as a model protein, and by the long-term culture of the marine microalga Nannochloropsis gaditana under practical conditions. The results from both methods were similar, confirming that the BSA test predicts microalgal biofouling on surfaces exposed to microalgae cultures; these secrete macromolecules, such as proteins, that have a high capacity for forming a conditioning film prior to cell adhesion. The hydrogel-based coating showed significantly reduced BSA and N. gaditana adhesion, whereas the other surfaces failed to control biofouling. Microalgal biofouling was associated with an increased concentration of sticky extracellular proteins at low N/P ratios (below 15).

show abstract

Long‐term biofouling formation mediated by extracellular proteins in Nannochloropsis gaditana microalga cultures at different medium N/P ratios

Soriano-Jerez

López-Rosales

Cerón-García

et al. 2020

Biotech & Bioengineering

View full text Add to dashboard Cite

Biofouling represents an important limitation in photobioreactor cultures. The biofouling propensity of different materials (polystyrene, borosilicate glass, polymethyl methacrylate, and polyethylene terephthalate glycol‐modified) and coatings (two spray‐applied and nanoparticle‐based superhydrophobic coatings and a hydrogel‐based fouling release coating) was evaluated by means of a short‐term protein test, using bovine serum albumin (BSA) as a model protein, and by the long‐term culture of the marine microalga Nannochloropsis gaditana under practical conditions. The results from both methods were similar, confirming that the BSA test predicts microalgal biofouling on surfaces exposed to microalgae cultures whose cells secrete macromolecules, such as proteins, with a high capacity for forming a conditioning film before cell adhesion. The hydrogel‐based coating showed significantly reduced BSA and N. gaditana adhesion, whereas the other surfaces failed to control biofouling. Microalgal biofouling was associated with an increased concentration of sticky extracellular proteins at low N/P ratios (below 15).

show abstract

A methodological study of adhesion dynamics in a batch culture of the marine microalga Nannochloropsis gaditana

Cited by 21 publications

References 43 publications

New insights into developing antibiofouling surfaces for industrial photobioreactors

New insights into developing antibiofouling surfaces for industrial photobioreactors

Long-term biofouling formation mediated by extracellular proteins in Nannochloropsis gaditana microalga cultures at different medium N/P ratios

Long‐term biofouling formation mediated by extracellular proteins in Nannochloropsis gaditana microalga cultures at different medium N/P ratios

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