A methodology for analysis of tissue sulfhydryl components

Assessment of rubber tree panels under crowns resistant to South American leaf blight

“…The Mg content in the latex was assessed in the supernatant diluted in 2.5% trichloroacetic acid (Moraes & Moraes, 1997). Sucrose was determined using the anthrone method (Ashwell, 1957), while thiols (R-SH), BIL, and Pi were evaluated according to the methods described by Boyne & Ellman (1972), Ribaillier (1968), andTausski &Shorr (1953), respectively. The circumference of the trunk was measured and virgin bark samples were collected 1.3 m above ground level to determine the number of latex vessel rings, density and diameter of laticiferous vessels, bark thickness, and average distance between consecutive latex vessel rings.…”

Protective effects of compound FLZ, a novel synthetic analogue of squamosamide, on β-amyloid-induced rat brain mitochondrial dysfunction in vitro

“…The reaction in 1 mL of the incubation buffer (100 mmol/L sucrose,100 mmol/L KCl, 2 mmol/L KH 2 PO 4 , 10 μmol/L EGTA, 5 mmol/L HEPES, pH 7.4) containing 0.15 mg of mitochondria protein was initiated by continuous stirring at 30 °C. Aβ [25][26][27][28][29][30][31][32][33][34][35] was incubated with mitochondria for 5 min before the addition of succinate. Mitochondrial swelling was assayed by monitoring light scattering at 520 nm for 30 min on a spectrophotometer.…”

“…Thus, FLZ possesses a strong neuroprotective property [12] . In addition, FLZ was also shown to reduce the impairment in learning and memory and the damage to the hippocampus induced by intracerebroventricular (icv) injection of Aβ [25][26][27][28][29][30][31][32][33][34][35] in mice. In SH-SY5Y cells, FLZ was also shown to inhibit apoptosis induced by Aβ 25-35 [14, 15] .…”

“…In a recent study, FLZ was effective in protecting against LPSand MPTP-induced neurotoxicity in dopaminergic neurons and mice [16] . Based on these results, the present study examines whether FLZ can reduce Aβ [25][26][27][28][29][30][31][32][33][34][35] -induced dysfunction of isolated rat brain mitochondria.…”

La réponse à la stimulation des clones d\'une même classe métabolique varie chez <i>Hevea brasiliensis</i> Muell Arg. (Euphorbiacea)

“…La production de caoutchouc sec a été estimée, en grammes par arbre (g/a), à partir la moyenne des productions mensuelles. Les paramètres biochimiques suivants : le saccharose (Sac) (Ashwel, 1957), le phosphore inorganique (Pi) (Taussky et Shorr, 1953), l'extrait sec (ExS) (Eschbach et al, 1984), les groupements thiols (RSH) (Boyne et Ellman, 1972) ont été mesurés à partir du latex des clones étudiés. Les quinze arbres de chaque clone ont été répartis en trois groupes de cinq arbres.…”