A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen

Wild Crop Relatives: Genomic and Breeding Resources

“…Methods to cryopreserve cassava germplasm were developed by Escobar et al (1997) using classical protocols (chemical dehydration and programmed freezing). Methods to cryopreserve cassava germplasm were developed by Escobar et al (1997) using classical protocols (chemical dehydration and programmed freezing).…”

Plant Cryopreservation: A Practical Guide

“…In carnation, not only the apex itself was superior but also the four uppermost nodal explants gave similar and better values than the nodes below ). Shoot tips were best for sweet potato (Pennycooke and Towill 2000), potato , cassava (Escobar et al 1997). This may be correlated with apical dominance effects and the resulting state and size of the buds (Pennycooke and Towill 2000).…”

“…The first publication of cassava cryopreservation was by Bajaj (1977b. Stem cuttings are used for primary explants (Kartha et al 1982;Escobar et al 1997). A minor tuber crop is the Madagascar potato [Solenostemon rotundifolius (Poir.)…”

“…Droplet freezing on aluminum foil; 15% DMSO Grout and Crisp 1995 Controlled rate cooling, various speed steps Kartha et al 1982;Escobar et al 1997Vitrification PVS2 Charoensub et al 1999Escobar et al 2000;Ng and Ng 2000 Freezing with filter paper wrapped in aluminium foil, 10% DMSO…”

“…It is sometimes combined with ABA or sorbitol (Escobar et al 1997). Generally vitrification methods start with overnight incubation of the explants either on growth medium or on increased sucrose concentrations like in mint (Towill 1988) and sometimes the incubation on high sucrose is prolonged up to 2 days.…”