A micro flow injection electrochemical biosensor for organophosphorus pesticides

Neufeld, Tova; Eshkenazi, Inna; Cohen, E.; Rishpon, Judith

doi:10.1016/s0956-5663(00)00073-7

Cited by 89 publications

(23 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the last case, the electrochemical mediator can be adsorbed on the working electrode surface such as in the case of Prussian Blue [33], cobalt hexacyanoferrate [34] or it can be directly mixed to the ink used to print the working electrode, such as in the case of cobalt phthalocyanine (CoPc) [35]. In the case of Au-SPEs modified with SAM, our choice was to use the electrochemical mediator ferricyanide in solution because it is able to electrocatalyse the oxidation of thiocholine [36].…”

Section: Amperometric Thiocholine Detection At Au-spe By Means Of Fermentioning

confidence: 99%

“…Amperometric thiocholine detection at Au-SPE modified by means of ferricyanide 3.2.1. Choice of applied potential A plot of current/applied potential using a thiocholine and ferricyanide concentration of 3 × 10 −4 M and 1 mM respectively [36], was constructed in the range of the applied potential 0-800 mV vs. Ag/AgCl (data not shown). As expected, the amperometric response followed the behaviour of the oxidation current in the CV.…”

Section: Amperometric Thiocholine Detection At Au-spe By Means Of Fermentioning

confidence: 99%

“…Using the previous selected applied potential (+400 mV vs. Ag/AgCl) and a ferricyanide concentration equal to 1 mM [36], calibration curves were carried out obtaining a detection limit (S/N = 3) of 3 × 10 −6 M together with a linear range up to 1 × 10 −3 M (R 2 = 0.9964). The sensor showed also a sensitivity equal to 113 mA M −1 cm −2 , which is higher than the one shown by the SPE modified with CoPc (24 mA M −1 cm −2 ) and comparable with that obtained in the case of SPE modified with Prussian Blue (143 mA M −1 cm −2 ) [13].…”

Section: Analytical Features Of Thiocholine Measurementmentioning

confidence: 99%

See 2 more Smart Citations

Acetylcholinesterase biosensor based on self-assembled monolayer-modified gold-screen printed electrodes for organophosphorus insecticide detection

Arduini

Guidone

Amine

et al. 2013

Sensors and Actuators B: Chemical

117

View full text Add to dashboard Cite

a b s t r a c tA mono-enzymatic acetylcholinesterase (AChE) amperometric biosensor for organophosphate detection was developed immobilizing the AChE enzyme via glutaraldehyde on a preformed cysteamine selfassembled monolayer (SAM) on gold-screen printed electrodes (Au-SPEs). The enzymatic activity was monitored measuring the enzymatic product, thiocholine, at an applied potential of +400 mV vs. Ag/AgCl using ferricyanide in solution as electrochemical mediator. The electrocatalytic activity of ferricyanide towards thiocholine was investigated by using Nicholson-Shain method and finding a second order homogenous rate constant k s equal to (5.26 ± 0.65) × 10 4 M −1 s −1 . In order to develop a sensitive biosensor, the effect of cysteamine concentration, duration of SAM deposition and AChE concentration were optimized. Using paraoxon as model compound, the biosensor showed a linear range up to 40 ppb with a detection limit of 2 ppb (10% of inhibition). The biosensor was successfully challenged with drinking water sample demonstrating to be a useful analytical tool for organophosphorus insecticide detection.

show abstract

Section: Amperometric Thiocholine Detection At Au-spe By Means Of Fermentioning

confidence: 99%

Section: Amperometric Thiocholine Detection At Au-spe By Means Of Fermentioning

confidence: 99%

Section: Analytical Features Of Thiocholine Measurementmentioning

confidence: 99%

See 1 more Smart Citation

Acetylcholinesterase biosensor based on self-assembled monolayer-modified gold-screen printed electrodes for organophosphorus insecticide detection

Arduini

Guidone

Amine

et al. 2013

Sensors and Actuators B: Chemical

117

View full text Add to dashboard Cite

show abstract

“…In these cases the measurement of the residual enzyme activity resulted in a log ν linear with the incubation time. Neufeld et al (2000), Guerrieri et al (2002) have reported that the degree of inhibition also depends on the inhibitor concentration and exposure time (at a defined pH value and at inhibitor concentration which is in excess with respect to enzyme).…”

Section: Irreversible Inhibitionmentioning

confidence: 99%

Enzyme inhibition-based biosensors for food safety and environmental monitoring

Amine¹,

Mohammadi²,

Bourais³

et al. 2006

Biosensors and Bioelectronics

552

263

View full text Add to dashboard Cite

“…Organophosphorus pesticides (OP) are very toxic compounds, they can lead to a severe impairment of nerve functions of human or even death because they inhibit main metabolic pathways (Ivanov et al, 2002, Neufeld et al, 2000. The intensive use of organophosphorus pesticides in agriculture requires further progress in the development of rapid and efficient monitoring methods.…”

Section: Introductionmentioning

confidence: 99%

Study on Carrier Material of Immobilization Acetylcholinesterase For Biosensor in Detectionof Organophosphorus Pesticide Residues

Sun

Wang

Chuan-dong³

2009

Computer and Computing Technologies in Agriculture II, Volume 3

View full text Add to dashboard Cite

Abstract:A comparison between several immobilization materials of AChE on surface of glassy carbon electrode(GCE) was presented. The immobilization methods employed crosslinking method with glutaraldehyde as a cross-linking agent and bovine serum albumin(BSA) as a protectant, AChE was immobilized on different membranes including nylon membrane, cellulose nitrate membrane and chitosan membrane respectively. The enzyme membrane was then fixed on the surface of glassy carbon electrode(GCE) with O-ring to prepare an amperometric biosensor for the detection of organophosphorus pesticides. The activity of immobilization AChE was detected by measuring the oxidation current of thiocholine, the results showed that the activity of immobilization AChE were all different with different membrane as carrier material. Compared with nylon membrane and cellulose nitrate membrane, chitosan membrane was obviously good. So chitosan membrane can be selected as immobilized AChE carrier material.

show abstract

A micro flow injection electrochemical biosensor for organophosphorus pesticides

Cited by 89 publications

References 19 publications

Acetylcholinesterase biosensor based on self-assembled monolayer-modified gold-screen printed electrodes for organophosphorus insecticide detection

Acetylcholinesterase biosensor based on self-assembled monolayer-modified gold-screen printed electrodes for organophosphorus insecticide detection

Enzyme inhibition-based biosensors for food safety and environmental monitoring

Study on Carrier Material of Immobilization Acetylcholinesterase For Biosensor in Detectionof Organophosphorus Pesticide Residues

Contact Info

Product

Resources

About