A microassay for antibody binding to tumor cell surface antigens using 125I-labelled protein a from Staphylococcus aureus

Brown, Joseph P.; Klitzman, Jack M.; Hellström, Karl Erik

doi:10.1016/0022-1759(77)90017-5

Cited by 72 publications

(27 citation statements)

References 21 publications

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“…On the other hand, specific immunity acquired by tumour excision is mediated by both cytotoxic cells (Belehradek et al, 1972) and antibody (Pilch & Riggins, 1966). By using a modification of the method described by Brown et al (1977), sera from Hf1 tumour-bearing mice and from mice immunized by tumour excision were found to possess specific antibody to the HI tumour (Kearney, to be published). However, similar antibody activity could not be found in sera of mice immunized by MCT-H 1 cells.…”

Section: Discussionmentioning

confidence: 99%

Effects of carrageenan, PVP and tumour-bearer serum on immunity induced by excision or mitomycin C-treated tumour cells in mice

Kearney¹,

Wu²,

Orr³

1979

Br J Cancer

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Summary.-Carrageenan (Cg) was tested for its effects on the growth of, and immunity to, 2 methylcholanthrene-induced syngeneic murine fibrosarcomas (HI and H2). The tumours were found not to share major tumour-specific transplantation antigens. H2 appeared more immunogenic than HI. In contrast to HI, immunity induced by H2 was not affected by Cg, nor was its growth in Cg-treated normal mice augmented. We propose that TBS does not inhibit CMI in vivo provided that macrophages remain functional, but may do so when macrophages are rendered defective by antimacrophage agents or by products of neoplastic cells. Increasing the levels of specific effector cells can over-ride the inhibiting effects of TBS, even when defective macrophages are present.

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Section: Discussionmentioning

confidence: 99%

Effects of carrageenan, PVP and tumour-bearer serum on immunity induced by excision or mitomycin C-treated tumour cells in mice

Kearney¹,

Wu²,

Orr³

1979

Br J Cancer

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“…The described methods of an Immunobead rosette assay for the detection of anti body against platelets by direct and indirect marker for IgG antibody on cell surfaces [3,8,12,22]. However, in our experiments the use of protein A to demonstrate IgG binding to platelets was unsatisfactory be cause false positive reactions occurred con sistently and this effect has been noted by others [3,8], including nonspecific binding of protein A to platelet membrane.…”

Section: Discussionmentioning

confidence: 39%

“…However, in our experiments the use of protein A to demonstrate IgG binding to platelets was unsatisfactory be cause false positive reactions occurred con sistently and this effect has been noted by others [3,8], including nonspecific binding of protein A to platelet membrane.…”

Section: Discussionmentioning

confidence: 85%

Detection of Platelet Antibody Using Rosette Technique with Anti-IgG Antibody-Coated Polyacrylamide Gel

Salmassi¹,

Yokoyama²,

Maples³

et al. 1980

Vox Sanguinis

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Paraformaldehyde-fixed platelets from normal donors were used for detection of antibody to platelet by in vitro sensitization (indirect method) utilizing rabbit anti-human IgG heavy chain specific antibody-coated polyacrylamide gels (Immunobeads). The sensitized platelets formed rosettes with Immunobeads and the positive rosette count was over 30%, while control showed less than 8% when normal sera were used. This method was also applicable for detecting antibody-sensitized platelets in vivo (direct method) in patients with autoimmune thrombocytopenia. This method is simple, rapid and reproducible for clinical use. Direct and indirect immunofluorescent antibody tests and a blocking test with anti-human serum also supported the results of Immunobead rosetting technique.

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“…Deposits of immunoglobulins were seen on the surface of cells in kerato-acanthoma (Brown et al, 1977).…”

Section: Discussionmentioning

confidence: 98%

Morphometric analysis and identification of infiltrating leucocytes in regressing and progressing shope rabbit papillomas

Okabayashi¹,

Angell²,

Christensen³

et al. 1991

Intl Journal of Cancer

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Spontaneous regressions of papillomavirus lesions frequently occur in both human and animal infections. The mechanism by which this occurs is currently unknown. Mononuclear infiltrates are found in regressing human and rabbit papillomas. To assess the potential functional role of these infiltrates in regression, we have characterized and quantitated the cell types present in regressing rabbit lesions. Forty New Zealand white rabbits were inoculated with cottontail rabbit papillomavirus (CRPV) at 2 sites on the dorsal skin. All tumors on 6 rabbits markedly decreased in volume within 6 to 8 weeks of inoculation. Tumors on 4 of these 6 regressor rabbits were studied by immunohistochemistry. Regressor papillomas had conspicuous leucocytic infiltrates, most concentrated at the epithelial basement membrane, and often obliterating the basal cells of the germinal layer. Infiltrating leucocytes were also concentrated in the subjacent dermis immediately beneath the basement membrane. The infiltrates gradually lessened at increased depths in the dermis. In contrast, progressor papillomas contained fewer leucocytes, which were randomly distributed in the dermis. The phenotype of the infiltrating leucocytes was examined in 4 regressing and 12 progressing papillomas. In regressing papillomas, infiltrating leucocytes were predominantly T cells (68.0%), with relatively few B cells (7.4%). Progressing papilloma dermis contained fewer T cells and B cells than regressing papillomas. Most of the infiltrating T cells in regressing papillomas were labelled with a rabbit MHC-class-II-specific monoclonal antibody (MAb) (2C4), in contrast to only a small number in progressing papillomas. In addition to the leucocytic infiltrates, keratinocytes in regressing, but not in progressing, papillomas, frequently exhibited strong 2C4 staining. These results demonstrate that infiltration with T cells expressing rabbit class II is characteristic of regressing Shope papillomas and strengthens the assertion that cell-mediated immunity is the mechanism of Shope papilloma regression.

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A microassay for antibody binding to tumor cell surface antigens using 125I-labelled protein a from Staphylococcus aureus

Cited by 72 publications

References 21 publications

Effects of carrageenan, PVP and tumour-bearer serum on immunity induced by excision or mitomycin C-treated tumour cells in mice

Effects of carrageenan, PVP and tumour-bearer serum on immunity induced by excision or mitomycin C-treated tumour cells in mice

Detection of Platelet Antibody Using Rosette Technique with Anti-IgG Antibody-Coated Polyacrylamide Gel

Morphometric analysis and identification of infiltrating leucocytes in regressing and progressing shope rabbit papillomas

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