A microassay for quantification of 2,2-diphenyl-1-picrylhydracyl (DPPH) free radical scavenging

MelÃ©ndez, Norma P.; NevÃ¡rez-MoorillÃ3n, Virginia; RodrÃ­guez-Herrera, RaÃol; Espinoza, JosÃ© C.; Aguilar, CristÃ3bal NoÃ©

doi:10.5897/ajbr2013.0669

Cited by 26 publications

(5 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Antioxidant activity was investigated using DPPH microassay as it was described by Melendez et al with some modifications [31]. Diluted samples and standard solutions (7 µL) were allowed to react with 193 µL of the DPPH radical solution (1.86 × 10 −4 mol L −1 DPPH in ethanol, prepared ex tempore) in 96-well microplate in triplicate.…”

Section: 2'-diphenyl-1-picrylhydrazyl (Dpph) Radical Scavenging Microassaymentioning

confidence: 99%

Impact of Deep Eutectic Solvents on Extraction of Polyphenols from Grape Seeds and Skin

et al. 2020

View full text Add to dashboard Cite

In the past few years, research efforts have focused on plant exploitation for deriving some valuable compounds. Extraction has been usually performed using petrochemical and volatile organic solvents, but nowadays, increased recognition of environmental pollution has prompted the utilization of green solvents as alternatives. Therefore, the aim of the present study was to exploit deep eutectic solvents (DES) (choline chloride: citric acid and choline chloride: glucose) as solvents for extracting valuable phenolic antioxidants from grapes. Investigation was conducted on ten grape varieties, observing seeds and skin as different matrix. Total polyphenol content (TPC) was determined by Folin-Ciocalteu spectrophotometric microassay. Antioxidant activity was investigated using four different tests and results were combined in a unique Antioxidant Composite Index (ACI) to reveal comprehensive information about this biological activity. Polyphenol compounds were identified and quantified with the aim of HPLC-diode array detector (DAD). Overall results support that DES (particularly choline chloride: citric acid) were comparable to conventional solvent, and in most cases even outperformed acidified aqueous ethanol (concerning extraction efficiency and antioxidant activity). Regardless of varietal distinctions, grape seeds have higher antioxidant capacity compared to grape skin, and such findings are according to their phenol compound concentrations.

show abstract

Section: 2'-diphenyl-1-picrylhydrazyl (Dpph) Radical Scavenging Microassaymentioning

confidence: 99%

Impact of Deep Eutectic Solvents on Extraction of Polyphenols from Grape Seeds and Skin

et al. 2020

View full text Add to dashboard Cite

show abstract

“…DPPH radical scavenging ability was estimated as has been described with some modifications [ 32 ]. Diluted samples (100 times) and standard solutions (7 µL) were mixed with 193 µL of the DPPH radical solution (1.86 × 10 −4 mol L −1 DPPH in ethanol, prepared ex tempore) in MTP wells.…”

Section: Methodsmentioning

confidence: 99%

Optimization of Extraction and HPLC–MS/MS Profiling of Phenolic Compounds from Red Grape Seed Extracts Using Conventional and Deep Eutectic Solvents

et al. 2022

View full text Add to dashboard Cite

Winemaking generates large quantities of grape waste consisting of seeds, skin and stalks. Given that grape seeds are a rich source of different bioactive compounds, the main goal of this research was to optimize grape seed phenol extraction using a Box–Behnken design. The following conditions were derived from the optimization process: sample:solvent ratio of 1:10 w/v, extraction time of 30 min and extraction temperature of 50 °C. In addition, a sustainable (green) approach for obtaining extracts was developed by comparing choline chloride:citric acid-ChCit (natural deep eutectic solvent (NADES)) and ethanol extraction methods with respect to phenol profiles and antioxidant activity. This study was conducted on seeds from eight different red grape varieties. Phenolic acids, flavan-3-ols and procyanidins were characterized using HPLC–MS/MS, and the concentration of procyanidin B1 was above 1 mg/g of dry weight in all analyzed samples. The contents of all phenol classes and antioxidant activities were found to not differ significantly between the solvents, but NADES was found to offer valuable advantages. Importantly, ChCit showed a strong affinity toward procyanidins and a strong correlation between antioxidant activity and quantified phenolic compounds.

show abstract

“…The antioxidant activity of the extracts was evaluated based on the scavenging activity of the 2,2-diphenyl-1-picrylhydrazyl (DPPH, Sigma-Aldrich ® ) free radical, as described by Meléndez et al [18]. Briefly, a reaction mixture consisting of 7 µL of the extract and 193 µL of 60 µM DPPH in absolute methanol was analyzed on a BioTek ® Microplate reader (ELx808™, Santa Clara, CA, USA) with absorbance filters for a wavelength of 520 nm.…”

Section: Analytical Analysismentioning

confidence: 99%

Improved Extraction of High Value-Added Polyphenols from Pomegranate Peel by Solid-State Fermentation

Buenrostro-Figueroa¹,

Nevárez-Moorillón

Chávez‐González

et al. 2023

Fermentation

View full text Add to dashboard Cite

Pomegranate peel is an important source of polyphenols of remarkable interest in the food, pharmaceutical and cosmetic industry. The improved extraction of total polyphenolic compounds (TPC) from pomegranate peel by solid-state fermentation (SSF) was achieved. The Box, Hunter and Hunter (BHH) followed by the central composite design (CCD) processes were performed to assess the effect of the process variables on TPC release. The statistical designs indicate that the best TPC extraction (234.85 mg GAE/gdm) by means of SSF occurs at 42 °C, 50% moisture, 5.0 pH, mineral solution (g/L): NaNO3 (3.83), KH2PO4 (1.52), MgSO4 (4.66) and KCl (1.52) at 36 h. Under the best fermentation conditions TPC (248.78 ± 1.24 mgGAE/gdm) increased 5.96-fold more than values previously reported and antioxidant activity (AA) increased 5.81-fold compared to the value obtained before the SSF optimization. High-value citric acid, α and β punicalin, α and β punicalagin, punigluconin, galloyl-HHDP hexoside and ellagic acid molecules were identified. The increased extraction of TPC by SSF provides a suitable alternative for the valorization of pomegranate peel through the recovery of molecules with high added value with potential use in the food, pharmacy and cosmetic industries; a diversification in the use of food agroindustry by-products is obtained as an approach to the circular economy model through biotechnological processes.

show abstract

A microassay for quantification of 2,2-diphenyl-1-picrylhydracyl (DPPH) free radical scavenging

Cited by 26 publications

References 15 publications

Impact of Deep Eutectic Solvents on Extraction of Polyphenols from Grape Seeds and Skin

Impact of Deep Eutectic Solvents on Extraction of Polyphenols from Grape Seeds and Skin

Optimization of Extraction and HPLC–MS/MS Profiling of Phenolic Compounds from Red Grape Seed Extracts Using Conventional and Deep Eutectic Solvents

Improved Extraction of High Value-Added Polyphenols from Pomegranate Peel by Solid-State Fermentation

Contact Info

Product

Resources

About