A Microbial Electrochemical Technology to Detect and Degrade Organophosphate Pesticides

Karbelkar, Amruta A.; Reynolds, Erin. E.; Ahlmark, Rachel; Furst, Ariel L.

doi:10.1021/acscentsci.1c00931

Cited by 37 publications

(20 citation statements)

References 76 publications

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“…The synthesized compounds outperformed the organophosphorus pesticide that was prescribed. 21 Insects find flu oncoated walls extremely slippery, as aggregates of PTFE particles detach from the surfaces and adhere to their pads. Climbing ants, on the other hand, can remove Fluon coatings from the walls of their nest containers (A.F.…”

Section: Introductionmentioning

confidence: 99%

Preparation of polyurethane coating formulation based on dihydropyridine derivatives as an insecticide and antifungal additives for surface coating applications

et al. 2022

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Pyridine derivatives are prepared and evaluated before being incorporated into polyurethane coating formulations to create antifungal and insecticidal coating compositions. Different analyses, including Fourier transform infrared (FTIR), mass, proton nuclear magnetic resonance (1HNMR), and carbon-13 nuclear magnetic resonance (13C NMR) spectra, were used to confirm the synthesized compounds. The material has been coated using a polyurethane coating mixture. Gloss, scratch resistance, flexibility, and adhesion are some of the coating attributes investigated; mechanical capabilities include impact resistance and shore hardness, and physicochemical properties such as chemical resistance of coated polyurethane (PU) samples are also investigated. PU coatings were applied to substrates to measure coating properties. The mechanical properties of the PU cast films were measured. The results of the experiments revealed that all PU coatings based on dihydropyridine derivatives had good scratch resistance which varied from > 1.5 to > 2 kg. While reducing gloss value varied from 65 to 85, there is no effect of the prepared compounds in the other mechanical test. These PU coatings have excellent chemical resistance except the alkali resistance as evidenced by their physicochemical properties. The observed antifungal and insecticide activities indicated that dry wood coated with PU based on dihydropyridine derivatives is promising for resistance to these insects and fungi, in comparison with the paint as blank. The results revealed that the inhibition zones diameter by compound 2 were 25.1 ± 0.69, 23.2 ± 0.94, 20.16 ± 0.62, 20 ± 0.80, and 18 ± 0.81 mm against A. terreus, A. niger, A. flavus, C. albicans, and A. fumigatus, respectively, whereas the inhibition zones (IZ) diameter by compound 3 were 22.56 ± 0.30, 21.03 ± 0.49, 21.03 ± 0.61, 21 ± 0.66, and 20 ± 0.78 mm versus A. niger, A. fumigatus A. flavus, C. albicans, and A. terreus, respectively. The ordering activity against insects increased as the dose concentration of the pyridine derivatives was increased.

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Section: Introductionmentioning

confidence: 99%

Preparation of polyurethane coating formulation based on dihydropyridine derivatives as an insecticide and antifungal additives for surface coating applications

et al. 2022

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“…Many microbes are of interest for bioelectrochemical systems to perform sustainable transformations including wastewater treatment and carbon capture as well as to act as bioengineered electrochemical sensors. 67 However, these species can require significant lengths of time to colonize electrodes and form biofilms. 68 One species, in particular, Shewanella oneidensis, is considered a model organism for cells interfacing with electrodes.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Carbon Electrode-Based Biosensing Enabled by Biocompatible Surface Modification with DNA and Proteins

et al. 2023

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Modification of electrodes with biomolecules is an essential first step for the development of bioelectrochemical systems, which are used in a variety of applications ranging from sensors to fuel cells. Gold is often used because of its ease of modification with thiolated biomolecules, but carbon screen-printed electrodes (SPEs) are gaining popularity due to their low cost and fabrication from abundant resources. However, their effective modification with biomolecules remains a challenge; the majority of work to-date relies on nonspecific adhesion or broad amide bond formation to chemical handles on the electrode surface. By combining facile electrochemical modification to add an aniline handle to electrodes with a specific and biocompatible oxidative coupling reaction, we can readily modify carbon electrodes with a variety of biomolecules. Importantly, both proteins and DNA maintain bioactive conformations following coupling. We have then used biomolecule-modified electrodes to generate microbial monolayers through DNA-directed immobilization. This work provides an easy, general strategy to modify inexpensive carbon electrodes, significantly expanding their potential as bioelectrochemical systems.

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“…Employing live cellular actuators in synthetic polymer networks allows the cross-linking chemistry to remain independent of the input signal and logical architecture. This orthogonality vastly broadens the scope of inputs and computations that can regulate a cross-linking event, including signals such as specific DNA/RNA sequences 50,51 , clinical biomarkers 56,57 , or environmental contaminants 58 . Applying recent advancements in genetic circuit standardization, such as gate matching 37 , could further tune network performance and sensitivity to tailor the cross-linking response from signal concentration-dependent (analog) to binary (digital).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional Regulation of Synthetic Polymer Networks

Graham

Dundas

Partipilo

et al. 2021

Preprint

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Individual cells direct non-equilibrium processes through coordinated signal transduction and gene expression, allowing for dynamic control over multicellular, system-wide behavior. This behavior extends to remodeling the extracellular polymer matrix that encases biofilms and tissues, where constituent cells dictate spatiotemporal network properties including stiffness, pattern formation, and transport properties. The majority of synthetic polymer networks cannot recreate these phenomena due to their lack of autonomous centralized actuators (i.e., cells). In addition, non-living polymer networks that perform computation are generally restricted to a few inputs (e.g., light, pH, enzymes), limiting the logical complexity available to a single network chemistry. Toward synergizing the advantages of living and synthetic systems, engineered living materials leverage genetic and metabolic programming to establish control over material-wide properties. Here we demonstrate that a bacterial metal respiration mechanism, extracellular electron transfer (EET), can control metal-catalyzed radical cross-linking of polymer networks. Linking metabolic electron flux to a synthetic redox catalyst allows dynamic, tunable, and predictable control over material formation and bulk polymer network mechanics using genetic circuits. By programming key EET genes with transcriptional Boolean logic, we rationally design computational networks that sense-and-respond to multiple inputs in biological contexts. Finally, we capitalize on the wide reactivity of EET and redox catalyses to predictably control another class of living synthetic materials using copper(I) alkyne-azide cycloaddition click chemistry. Our results demonstrate the utility of EET as a bridge for controlling abiotic materials and how the design rules of synthetic biology can be applied to emulate physiological behavior in polymer networks.

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A Microbial Electrochemical Technology to Detect and Degrade Organophosphate Pesticides

Cited by 37 publications

References 76 publications

Preparation of polyurethane coating formulation based on dihydropyridine derivatives as an insecticide and antifungal additives for surface coating applications

Preparation of polyurethane coating formulation based on dihydropyridine derivatives as an insecticide and antifungal additives for surface coating applications

Carbon Electrode-Based Biosensing Enabled by Biocompatible Surface Modification with DNA and Proteins

Transcriptional Regulation of Synthetic Polymer Networks

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