A microchip electrophoresis device with on-line microdialysis sampling and on-chip sample derivatization by naphthalene 2,3-dicarboxaldehyde/2-mercaptoethanol for amino acid and peptide analysis

Huynh, Bryan H.; Fogarty, Barbara A.; Nandi, Pradyot; Lunte, Susan M.

doi:10.1016/j.jpba.2006.05.014

Cited by 42 publications

(29 citation statements)

References 38 publications

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“…Advantages of using such devices for electrophoresis are linked to easier automation of processes, which leads to a high degree of process integration in biological systems [72]. Excellent reviews by Giles and coworkers [73], Khandurina and coworkers [74], Figeys and coworkers [75], and Guihen [76] have documented the developments in this area.…”

Section: A Microfluidic Toolbox For Ispr Developmentmentioning

confidence: 99%

A Microfluidic Toolbox for the Development of In-Situ Product Removal Strategies in Biocatalysis

Heintz¹,

Mitić²,

Ringborg³

et al. 2016

J Flow Chem

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A microfluidic toolbox for accelerated development of biocatalytic processes has great potential. This is especially the case for the development of advanced biocatalytic process concepts, where reactors and product separation methods are closely linked together to intensify the process performance, e.g., by the use of in-situ product removal (ISPR). This review provides a general overview of currently available tools in a microfluidic toolbox and how this toolbox can be applied to the development of advanced biocatalytic process concepts. Emphasis is placed on describing the possibilities and advantages of the microfluidic toolbox that are difficult to achieve with conventional batch-processbased technologies. Application of this microfluidic toolbox will potentially make it possible to intensify biocatalytic reactions and thereby facilitate the development towards novel and advanced biocatalytic processes, which in many cases have proven too difficult in conventional batch equipment.

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Section: A Microfluidic Toolbox For Ispr Developmentmentioning

confidence: 99%

A Microfluidic Toolbox for the Development of In-Situ Product Removal Strategies in Biocatalysis

Heintz¹,

Mitić²,

Ringborg³

et al. 2016

J Flow Chem

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“…Huynh et al [84] described the MCE device with on-line MD sampling for the analysis of AAs and peptide analysis, the glass chip was sealed with PDMS, using LIF detection, 12 fmol of 5 mM AA and peptide samples were introduced into the system. The AA and peptide mixtures were derivatized on-chip with naphthalene 2,3-dicarboxaldehyde and 2-mercaptoethanol for LIF detection.…”

Section: Recent Applications Of Mce In MDmentioning

confidence: 99%

Current separation and detection methods in microdialysis the drive towards sensitivity and speed

Guihen

O’Connor

2009

Electrophoresis

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This review outlines some of the analytical challenges associated with the analysis of microdialysis (MD) samples, in particular, the minute complex sample volumes that are often encountered. In MD sampling many different low-molecular-weight molecules can be collected, but the research findings are often limited by the sensitivity, specificity, and reliability of the analytical technique that is coupled to the dialysis probe. Therefore it is critical that a lot of consideration is given in selecting the most suitable analytical method including the most appropriate detector. This review aims to highlight the strengths and weaknesses of a range of commonly used analytical methods employed in MD. In Section 1, a brief overview of the MD technique is described, followed by a discussion on some of the advantages and drawbacks of this sampling technique. Sections 2 and 3 examine analytical and other technical considerations regarding analysis, with special emphasis on the factors that specifically influence analytical detection. Section 4 outlines the most commonly employed analytical techniques used in MD, including HPLC coupled with various detectors. Detail is given regarding the LOD and LOQ for many applications using each detector. As MS is of such high importance in MD, a special sub-section has been devoted to it. The importance of CE is also highlighted, with specific applications described. In addition, analytical techniques that do not appear to have found routine use in MD are discussed. Section 5 is concerned with recent innovations in chemical separation techniques, in particular MCE and ultra-performance liquid chromatography. Specific applications of the coupling of these techniques with MD are highlighted, along with technical challenges associated with miniaturization. In the Section 6, the future outlook of MD is discussed. Techniques other than electrophoretic- and chromatographic based separation methods are outside the scope of this review.

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“…The integrated rapid on-chip sample derivatization employing naphthalene 2,3-dicarboxyaldehyde (NDA) and 2-mercaptoethanol (2-ME) with an easily assembled microdialysis-chip electrophoresis was developed and tested as a prototype of micrototal analytical system for high-temporal resolution monitoring of amino acids and peptides in biological systems [77].…”

Section: Derivatizationmentioning

confidence: 99%

“…An example of a functional model of mTAS is a microchip CE device with on-line microdialysis sampling and on chipsample derivatization by NDA and 2-ME for LIF detection, which was developed to monitor peptides and amino acids in samples of biological origin, such as microdialysates from brain [77].…”

Section: Separation In Microfluidic Devicesmentioning

confidence: 99%

Recent developments in CE and CEC of peptides

Kašička

2007

Electrophoresis

118

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The article brings a comprehensive survey of recent developments and applications of high-performance capillary electromigration methods, zone electrophoresis, ITP, IEF, affinity electrophoresis, EKC, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides. New approaches to the theoretical description and experimental verification of electromigration behavior of peptides and to methodology of their separations, such as sample preparation, adsorption suppression, and detection, are presented. Novel developments in individual CE and CEC modes are shown and several types of their applications to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of CE and CEC techniques to provide important physicochemical characteristics of peptides are demonstrated.

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A microchip electrophoresis device with on-line microdialysis sampling and on-chip sample derivatization by naphthalene 2,3-dicarboxaldehyde/2-mercaptoethanol for amino acid and peptide analysis

Cited by 42 publications

References 38 publications

A Microfluidic Toolbox for the Development of In-Situ Product Removal Strategies in Biocatalysis

A Microfluidic Toolbox for the Development of In-Situ Product Removal Strategies in Biocatalysis

Current separation and detection methods in microdialysis the drive towards sensitivity and speed

Recent developments in CE and CEC of peptides

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