A microfluidic nanostructured fluorescence sensor for biomolecular binding detection

Li, Xiang; Yin, Haocheng; Que, Long

doi:10.1109/icsens.2013.6688281

Cited by 3 publications

(3 citation statements)

References 13 publications

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“…acidified poly(ethylene oxide) (PEO) [11]. Nanoscale networks/structures are often integrated within microchannels for a broad range of applications, such as electrophoretic separation of biomolecules [12,13], high reaction efficiency catalytic microreactors [14,15], and enhancement of heat transfer [16,17] and sensing [18,19]. Despite the investigations of EOF reduction due to nanostructured surfaces in microchannels [20][21][22][23], a proper study on the orientation effect of nanostructures is yet to be conducted.…”

Section: Introductionmentioning

confidence: 99%

Effect of nanostructures orientation on electroosmotic flow in a microfluidic channel

Lim

Lam

et al. 2017

Nanotechnology

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Electroosmotic flow (EOF) is an electric-field-induced fluid flow that has numerous micro-/nanofluidic applications, ranging from pumping to chemical and biomedical analyses. Nanoscale networks/structures are often integrated in microchannels for a broad range of applications, such as electrophoretic separation of biomolecules, high reaction efficiency catalytic microreactors, and enhancement of heat transfer and sensing. Their introduction has been known to reduce EOF. Hitherto, a proper study on the effect of nanostructures orientation on EOF in a microfluidic channel is yet to be carried out. In this investigation, we present a novel fabrication method for nanostructure designs that possess maximum orientation difference, i.e. parallel versus perpendicular indented nanolines, to examine the effect of nanostructures orientation on EOF. It consists of four phases: fabrication of silicon master, creation of mold insert via electroplating, injection molding with cyclic olefin copolymer, and thermal bonding and integration of practical inlet/outlet ports. The effect of nanostructures orientation on EOF was studied experimentally by current monitoring method. The experimental results show that nanolines which are perpendicular to the microchannel reduce the EOF velocity significantly (approximately 20%). This flow velocity reduction is due to the distortion of local electric field by the perpendicular nanolines at the nanostructured surface as demonstrated by finite element simulation. In contrast, nanolines which are parallel to the microchannel have no effect on EOF, as it can be deduced that the parallel nanolines do not distort the local electric field. The outcomes of this investigation contribute to the precise control of EOF in lab-on-chip devices, and fundamental understanding of EOF in devices which utilize nanostructured surfaces for chemical and biological analyses.

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Section: Introductionmentioning

confidence: 99%

Effect of nanostructures orientation on electroosmotic flow in a microfluidic channel

Lim

Lam

et al. 2017

Nanotechnology

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“…Nanostructures are commonly introduced in microchannels for various purposes, e.g., electrophoretic separation of biosamples [ 26 ], high efficiency microreactors [ 27 , 28 ], facilitation of heat transfer [ 29 , 30 ] and enhancement of sensing capability [ 31 , 32 ]. The presence of nanostructures in microchannels has been known to reduce EOF, when the nanostructures are against the EOF flow direction [ 33 , 34 , 35 , 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

Electroosmotic Flow in Microchannel with Black Silicon Nanostructures

Lim

Lam

et al. 2018

Micromachines

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Although electroosmotic flow (EOF) has been applied to drive fluid flow in microfluidic chips, some of the phenomena associated with it can adversely affect the performance of certain applications such as electrophoresis and ion preconcentration. To minimize the undesirable effects, EOF can be suppressed by polymer coatings or introduction of nanostructures. In this work, we presented a novel technique that employs the Dry Etching, Electroplating and Molding (DEEMO) process along with reactive ion etching (RIE), to fabricate microchannel with black silicon nanostructures (prolate hemispheroid-like structures). The effect of black silicon nanostructures on EOF was examined experimentally by current monitoring method, and numerically by finite element simulations. The experimental results showed that the EOF velocity was reduced by 13 ± 7%, which is reasonably close to the simulation results that predict a reduction of approximately 8%. EOF reduction is caused by the distortion of local electric field at the nanostructured surface. Numerical simulations show that the EOF velocity decreases with increasing nanostructure height or decreasing diameter. This reveals the potential of tuning the etching process parameters to generate nanostructures for better EOF suppression. The outcome of this investigation enhances the fundamental understanding of EOF behavior, with implications on the precise EOF control in devices utilizing nanostructured surfaces for chemical and biological analyses.

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“…Nanoscale networks/structures are often integrated within a microchannel for a broad range of applications, such as electrophoretic separation of biomolecules , Kaji et al 2004, high reaction efficiency catalytic microreactors (Kusakabe et al 2001, Miyazaki et al 2004, and enhancement of heat transfer (Nagayama et al 2007, Li et al 2012) and sensing (Li et al 2013, Ng et al 2016. Even though there are numerous investigations on EOF reduction due to the presence of nanostructures in microchannels (Kang and Suh 2009, Messinger and Squires 2010, Yasui et al 2011, Koga et al 2013, the effect of nanostructure orientation on EOF has yet to be examined.…”

Section: Research Motivationmentioning

confidence: 99%

Electroosmotic flow hysteresis and effect of nanostructure orientation in a microfluidic channel

Lim¹

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DTU Danchip (National Center for Micro-and Nanofabrication) for the assistance with the fabrication of the injection molded micro-/nanofluidic devices used in my research. I would also like to thank Ms. Wang Shu Rui and Mr. Chin Tian Kooi, Park Systems Pte Ltd, for the characterization of the fluidic devices with atomic force microscopy (AFM).Last but not least, I would like to give special thanks to my family, girlfriend and friends for their moral support and encouragement throughout my Ph.D. study.

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A microfluidic nanostructured fluorescence sensor for biomolecular binding detection

Cited by 3 publications

References 13 publications

Effect of nanostructures orientation on electroosmotic flow in a microfluidic channel

Effect of nanostructures orientation on electroosmotic flow in a microfluidic channel

Electroosmotic Flow in Microchannel with Black Silicon Nanostructures

Electroosmotic flow hysteresis and effect of nanostructure orientation in a microfluidic channel

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