A Micromachined Injection Device for CZE:  Application to Correlation CZE

Moolen, J.N. van der; Poppe, and Hans; Smit, H.C.

doi:10.1021/ac970403q

Cited by 48 publications

(36 citation statements)

References 50 publications

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“…Electrokinetic injection is one of the methods that can be used to randomly inject the sample and buffer solution. For example, this multiplex injection method has been demonstrated with cross-correlation CE using microchip technology (3,4). In addition, electrokinetic injection can be applied to several detection methods-absorbance, conventional fluorescence, and electrochemical.…”

Section: H Ha Ad Da Am Ma Ar Rd D T Tr Ra An Ns Sf Fo or Rm M C Ce E mentioning

confidence: 99%

Peer Reviewed: Hadamard Transform CE

Kaneta¹

2001

Anal. Chem.

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Section: H Ha Ad Da Am Ma Ar Rd D T Tr Ra An Ns Sf Fo or Rm M C Ce E mentioning

confidence: 99%

Peer Reviewed: Hadamard Transform CE

Kaneta¹

2001

Anal. Chem.

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“…This has been demonstrated for optical detection systems but has not yet been implemented on amperometric systems. Cross-correlation methods [20,21] are based on multiple injections. This does not need special hardware; however, the number of injections is extremely high.…”

Section: General Aspectsmentioning

confidence: 99%

Modified Hadamard transform microchip electrophoresis

Guchardi

Schwarz

2005

Electrophoresis

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Sensitivity is a crucial point in the development applications for medicine or environmental samples in which the analytes are present in the nanomolar range. Besides further technical development of detection systems, the multiplex sample injection technique can be applied for enhancing the signal-to-noise ratio. Hadamard transform is easily applied to microchip electrophoresis due to the fact that sample injection is generally achieved through cross, double-tee, or tee injector structures. This paper reports the first demonstration of a modified Hadamard transform electrophoresis on a microchip by using an amperometric detector. Contrary to the previous Hadamard applications, the resolution (number of points per unit of time) of electropherograms obtained is independent of the number of injections.

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“…In this technique, multiple sample injection according to a Hadamard code sequence derived from a Hadamard matrix was employed for CZE separation. Other studies involving crosscorrelation electrophoresis [14][15][16], Shah convolution Fourier transform electrophoresis [17][18][19], and modified HT microchip electrophoresis [20] have also been reported as techniques for improving the sensitivity in CZE using analogous mathematical transformations.…”

Section: Introductionmentioning

confidence: 99%

Application of Hadamard transformation to MEKC

2007

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The Hadamard transform (HT) technique, which permits the S/N in CE to be improved, was applied to MEKC. Multiple sample injection of fluorescent analytes according to a Hadamard code sequence was performed using an optically gated sample injection technique, in which a sample plug was produced based on photodegradation by irradiation with an intense laser beam. The capillary and reservoirs were filled with a sample solution containing buffer components and SDS as a pseudostationary phase. A preliminary study confirmed that fluorescein ion could be photobleached in the presence of SDS. The optically gated sample injection technique was then applied to multiple sample injection, based on a Hadamard matrix. The S/N in the electropherogram obtained by HT-MEKC was improved substantially compared to that obtained by a single injection method. When the technique was applied to the separation of several amino acids labeled with FITC, the S/N ratio for each amino acid was enhanced, without any evidence of degradation in separation resolution. Moreover, HT-MEKC was applied to the analysis of amino acids contained in a Japanese beverage, resulting in improved S/Ns for the amino acids.

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A Micromachined Injection Device for CZE: Application to Correlation CZE

Cited by 48 publications

References 50 publications

Peer Reviewed: Hadamard Transform CE

Peer Reviewed: Hadamard Transform CE

Modified Hadamard transform microchip electrophoresis

Application of Hadamard transformation to MEKC

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