A MINIATURIZED ENRICHMENT SEROLOGY METHOD FOR RAPID DETECTION of <i>SALMONELLA</i> FROM POULTRY MEAT and REARING FARMS ENVIRONMENT

Salvat, Gilles; Humbert, F.; Lalande, Françoise; Colin, P.; Lahellec, C.

doi:10.1111/j.1745-4581.1992.tb00088.x

Cited by 2 publications

(1 citation statement)

References 4 publications

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“…30 m l from each tube was inoculated into the corresponding tube in a fresh microplate containing 1·8 ml of M Broth and incubated overnight at 41°C. The following day, M broth tubes were used to perform the miniaturised enrichment serology (ES) technique adapted (Salvat et al, 1992) from the original description of Sperber and Deibel (1969).…”

Section: Numeration Method: Miniaturised Most Probable Number Methods mentioning

confidence: 99%

Antimicrobial efficacy of AvGard carcase wash under industrial processing conditions

Coppen¹,

Fenner²,

Salvat³

1998

British Poultry Science

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1. The efficacy of the AvGard Trisodium Phosphate (TSP) immersion carcase wash process was evaluated during 5 industrial trials against Salmonella, Enterobacteriaceae, thermotolerant coliforms and total aerobic count. The effect against Pseudomonas was also studied in the first 3 trials. 2. Dramatic reductions in Salmonella incidence were seen using a whole carcase rinse method. In 4 of the 5 trial sites, only one positive sample was found after AvGard treatment (average 0.5% incidence), in spite of an average control incidence of 57.7%. In the 5th site, a water-chilled broiler plant, an average control incidence of 74.0% was reduced to 9.4% after AvGard treatment. 3. In the latter case, Most Probable Number (MPN) analyses were performed on some of the Salmonella positive samples taken from the control and post-treatment series; the average MPN count per carcase on controls was 115, whereas for AvGard treated birds the figure was only 0.6 per carcase, a greater than 2 log reduction. 4. In addition, AvGard treatment gave average log reductions for all trials of: Enterobacteriaceae; 2.5 log; Coliforms; 2.7 log, and Total Aerobic Count; 1.1 log, leading to carcases substantially free of Gram negative pathogens. 5. Pseudomonas was reduced by an average of 1.7 log in the first 3 trials, dramatically reducing the carcase loading of this important spoilage organism.

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Section: Numeration Method: Miniaturised Most Probable Number Methods mentioning

confidence: 99%

Antimicrobial efficacy of AvGard carcase wash under industrial processing conditions

Coppen¹,

Fenner²,

Salvat³

1998

British Poultry Science

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Effects of avgard™ treatment on the microbiological flora of poultry carcases

Salvat¹,

Coppen²,

Allo³

et al. 1997

British Poultry Science

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1. The efficiency of the AvGard (or Assur-Rince in the USA) trisodium phosphate poultry carcase decontamination process was evaluated during both manual and industrial trials against total aerobic mesophilic count (TAMC), thermotolerant coliforms, Pseudomonas, Enterobacteriaceae, Campylobacter, Listeria monocytogenes and Salmonella. 2. The TSP treatment proved to have significant effects on the bacterial decontamination of poultry neck skin, lowering the contamination by a factor of about 10 for TAMC and of 100 for Coliform and Pseudomonas. 3. Numeration of Salmonella with an innovative miniaturised most probable number method has proved that the effect upon these micro-organisms was also close to 2 log10 reduction. 4. The effect of TSP treatment on the ecological balance of psychrotrophic bacterial flora was also investigated to study the origin of the shelf-life flora of treated carcases (Pseudomonas being reduced to the limit of detection) and to ascertain whether L. monocytogenes might gain a competitive advantage. In fact AvGard reduced the number of L. monocytogenes on poultry carcases. 5. As a consequence of the virtual elimination of the Pseudomonas usually present, Brochothrix thermosphacta became the main species responsible for putrefaction. 6. Because the growth rate of Brochothrix thermosphacta was greater than that of L. monocytogenes at refrigeration temperature, it was considered that putrefaction would occur before the emergence of large numbers of L. monocytogenes.

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A MINIATURIZED ENRICHMENT SEROLOGY METHOD FOR RAPID DETECTION of SALMONELLA FROM POULTRY MEAT and REARING FARMS ENVIRONMENT

Cited by 2 publications

References 4 publications

Antimicrobial efficacy of AvGard carcase wash under industrial processing conditions

Antimicrobial efficacy of AvGard carcase wash under industrial processing conditions

Effects of avgard™ treatment on the microbiological flora of poultry carcases

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