A minimal growth medium for Pseudomonas azotoformans associated with insect gut

Abstract

Pseudomonas spp. can be found associated with several insect hosts. Some of these strains play a crucial role in helping their hosts overcome biotic challenges such as plant defence effectors. Defined media and optimally minimal media are instrumental in studying the physiology and metabolic pathways of microorganisms. In the case of the insect-associated Pseudomonas azotoformans isolated from Galeruca laticollis' gut, we tested six previously designed defined/minimal media. To determine the optimal medium, we compared the bacterium's growth performances on the different defined/minimal media to its growth performance on a rich medium using the Dynamic Time Warping Algorithm. The growth results at both 25C and 30C indicated the SMM as the optimal medium, with bacterial growth performances being the closest to a rich medium. Therefore, this medium would be highly suitable to study the catabolic pathways for the degradation of some xenobiotics in P. azotoformans.