A minimally invasive, low-stress method for serial blood collection in aging mice

Bjorner, Marianne; Zhu, Lida

doi:10.1080/20010001.2019.1647400

Cited by 9 publications

(6 citation statements)

References 6 publications

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“…The blood samples collected in EDTA were used to perform a manual count of white blood cells using Turk’s solution ( Bjorner and Zhu, 2019 ). A 100-cell differential leucocyte counts (DWBC) was performed manually using a blood smear stained with Romanowsky solutions ( Blumenreich, 1990 ).…”

Section: Methodsmentioning

confidence: 99%

Seasonal variation of health in Asian elephants

Santos

Berger

Cristofari

et al. 2020

Conservation Physiology

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Long-lived species are often predicted to be buffered against seasonal variation: longevity means low annual mortality and reproductive rates and annual variability in climate may therefore have a smaller impact on population growth rates of long-lived species in comparison to short-lived ones. However, little is known of the physiological mechanisms underlying such patterns in long-lived species. In this study, we investigated seasonal variation in the health of Asian elephants living in a seasonal monsoon climate. We used two complementary methods: (i) global and (ii) trait-by-trait analyses of seasonal effects on 23 health parameters of 225 individually marked elephants with known age and reproductive and health history, with repeated measures per individual over a 26-month period. The global analysis highlighted the biggest differences in health between the hot and monsoon seasons. Our trait-specific analyses identified the physiological functions underlying such health variation in different ecological settings, including haematological, immunological, muscular, kidney and liver functions, as well as protein balance and electrolytes. Overall, the results suggest that even long-lived, large mammals may experience physiological changes in response to seasonal variation that in extreme circumstances can pose a significant health risk.

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Section: Methodsmentioning

confidence: 99%

Seasonal variation of health in Asian elephants

Santos

Berger

Cristofari

et al. 2020

Conservation Physiology

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“…Türk's solution consists of acetic acid, which is responsible for the hemolysis, and crystal violet to stain the remaining leukocytes, making them easier to see and count (16,33,34). Therefore, Türk's solution concentration test appeared to be an appropriate substitute to modify Knott's technique.…”

Section: Discussionmentioning

confidence: 99%

A comparison between different laboratory methods and stains for detection microfilaremic dogs

Al-Malachi¹,

Al-Farwachi²

2023

IJVS

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For microscopical and statistical evaluation of different laboratory methods and stains for detecting circulating microfilariae of Dirofilaria immitis in ten naturally infected local stray dog's positive to canine heartworm Ag ELISA kit. All blood samples were examined by three methods modified Knott's technique, serum concentration test, and Türk's solution concentration test and three laboratory stains methylene blue, giemsa, and acridine orange stains were used to dye the precipitate of the modified Knott's technique. Results showed that the slides prepared from the serum were easy to examine (there are no erythrocytes) compared with the slides prepared from the precipitate of the modified Knott's technique. The slides prepared using the Türk's solution were more transparent and freer of cell debris. The length and width of the microfilariae detected by the three methods did not change significantly. Our conclusion from this study is that Türk's solution concentration test and the serum concentration test can be used in place of the modified Knott's technique to detect microfilaremic dogs. Acridine orange staining can also substitute for methylene blue and giemsa stains for faster results.

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“…Specifically, blood was collected after a minimal puncture at the tail vein with a needle. 35 Individual mouse urine was aspirated and feces were collected after a gentle stimulus excretion and defecation on a clean disposable plastic container. 36 After the behavioral experiment, mice were fasted for 24 hours and weighed, and then sacrificed by cervical dislocation after femoral artery blood collection.…”

Section: Methodsmentioning

confidence: 99%