A miR-18a binding-site polymorphism in CDC42 3′UTR affects CDC42 mRNA expression in placentas and is associated with litter size in pigs

Liu, Ruize; Deng, Dadong; Liu, Xiangdong; Xiao, Yujing; Huang, Ji; Wang, Feiyu; Li, Xinyun; Yu, Mei

doi:10.1007/s00335-018-9788-x

Cited by 13 publications

(13 citation statements)

References 43 publications

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“…Indeed, miR-18a has already been associated with human fetal macrosomia [49] and preeclampsia [50]. Recently, it was shown that a single-nucleotide polymorphism (SNP) within a miR-18a binding site within CDC42 3 UTR lowers the ability of this miRNA to regulate and influence litter size by affecting oocyte maturation and placental development in pigs [51]. In addition, an SNP within a miR-27a gene was also found to be associated with pregnancy outcome.…”

Section: Mirnas At the Embryo-maternal Interfacementioning

confidence: 99%

MiRNAs in the Peri-Implantation Period: Contribution to Embryo–Maternal Communication in Pigs

Kaczmarek

Najmula

Guzewska

et al. 2020

IJMS

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MicroRNAs (miRNAs) constitute a large family of noncoding RNAs, approximately 22 nucleotides long, which function as guide molecules in RNA silencing. Targeting most protein-coding transcripts, miRNAs are involved in nearly all developmental and pathophysiological processes in animals. To date, the regulatory roles of miRNAs in reproduction, such as fertilization, embryo development, implantation, and placenta formation, among others, have been demonstrated in numerous mammalian species, including domestic livestock such as pigs. Over the past years, it appeared that understanding the functions of miRNAs in mammalian reproduction can substantially improve our understanding of the biological challenges of successful reproductive performance. This review describes the current knowledge on miRNAs, specifically in relation to the peri-implantation period when the majority of embryonic mortality occurs in pigs. To present a broader picture of crucial peri-implantation events, we focus on the role of miRNA-processing machinery and miRNA–mRNA infarctions during the maternal recognition of pregnancy, leading to maintenance of the corpus luteum function and further embryo implantation. Furthermore, we summarize the current knowledge on cell-to-cell communication involving extracellular vesicles at the embryo–maternal interface in pigs. Finally, we discuss the potential of circulating miRNAs to serve as indicators of ongoing embryo–maternal crosstalk.

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Section: Mirnas At the Embryo-maternal Interfacementioning

confidence: 99%

MiRNAs in the Peri-Implantation Period: Contribution to Embryo–Maternal Communication in Pigs

Kaczmarek

Najmula

Guzewska

et al. 2020

IJMS

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“…BCR plays a role in regulating small GTP-binding proteins, such as CDC42 (cell division cycle 42). CDC42 was detected to be expressed in the placenta of pigs and functions in epithelial tissue morphogenesis [74,75]. TIAM2 has been characterized to promote the cell proliferation and invasion [76,77].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and ChIP-seq Integrative Analysis Reveals Important Roles of Epigenetically Regulated lncRNAs in Placental Development in Meishan Pigs

Deng

Tan

Han

et al. 2020

Genes

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The development of the placental fold, which increases the maternal–fetal interacting surface area, is of primary importance for the growth of the fetus throughout the whole pregnancy. However, the mechanisms involved remain to be fully elucidated. Increasing evidence has revealed that long non-coding RNAs (lncRNAs) are a new class of RNAs with regulatory functions and could be epigenetically regulated by histone modifications. In this study, 141 lncRNAs (including 73 up-regulated and 68 down-regulated lncRNAs) were identified to be differentially expressed in the placentas of pigs during the establishment and expanding stages of placental fold development. The differentially expressed lncRNAs and genes (DElncRNA-DEgene) co-expression network analysis revealed that these differentially expressed lncRNAs (DElncRNAs) were mainly enriched in pathways of cell adhesion, cytoskeleton organization, epithelial cell differentiation and angiogenesis, indicating that the DElncRNAs are related to the major events that occur during placental fold development. In addition, we integrated the RNA-seq (RNA sequencing) data with the ChIP-seq (chromatin immunoprecipitation sequencing) data of H3K4me3/H3K27ac produced from the placental samples of pigs from the two stages (gestational days 50 and 95). The analysis revealed that the changes in H3K4me3 and/or H3K27ac levels were significantly associated with the changes in the expression levels of 37 DElncRNAs. Furthermore, several H3K4me3/H3K27ac-lncRNAs were characterized to be significantly correlated with genes functionally related to placental development. Thus, this study provides new insights into understanding the mechanisms for the placental development of pigs.

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“…Increasing evidence suggests that polymorphisms in the 3 UTRs of genes are associated with complex economic traits by affecting miRNA-related regulation gene expression [34][35][36]. For example, Clop et al demonstrated that a G-to-A transition in the 3 UTR of the myostatin gene generated a binding site for miR-1 and miR-206, which inhibited the translation of the myostatin gene and hence contributed to the muscular hypertrophy of Texel sheep [34].…”

Section: Discussionmentioning

confidence: 99%

A Functional 3′ UTR Polymorphism of FADS2 Affects Cow Milk Composition through Modifying Mir-744 Binding

Gao

et al. 2019

Animals

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This study determined the associations of FADS2 c.1571G>A with milk FAs content and revealed that cows with the GG genotype had improved levels of delta-6 desaturase substrates (linoleic acid, C18:2n-6; p < 0.001) and decreased levels of desaturase products (gamma-linolenic acid, C18:3n-6; p < 0.001), indicating a reduction in FADS2 expression or delta-6 desaturase activity caused by this polymorphism. Computer alignment demonstrated that c.1571G>A occurred within a potential miR-744 binding site. When the c.1571G allele was present, the luciferase activity of reporter constructs was significantly suppressed by miR-744, while no such effect was observed with the A allele. Overexpression of miR-744 in bovine mammary epithelial cells (with the 1571GG genotype) downregulated FADS2 expression at both mRNA and protein levels. In contrast, inhibition of endogenous miR-744 with a specific inhibitor dramatically upregulated FADS2 expression. Taken together, these lines of evidence indicated that the c.1571A minor allele abolished the ability of miR-744 to bind FADS2, with a consequent increase in FADS2 expression levels and synthesis of omega-6 LC-PUFAs.

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A miR-18a binding-site polymorphism in CDC42 3′UTR affects CDC42 mRNA expression in placentas and is associated with litter size in pigs

Cited by 13 publications

References 43 publications

MiRNAs in the Peri-Implantation Period: Contribution to Embryo–Maternal Communication in Pigs

MiRNAs in the Peri-Implantation Period: Contribution to Embryo–Maternal Communication in Pigs

Transcriptomic and ChIP-seq Integrative Analysis Reveals Important Roles of Epigenetically Regulated lncRNAs in Placental Development in Meishan Pigs

A Functional 3′ UTR Polymorphism of FADS2 Affects Cow Milk Composition through Modifying Mir-744 Binding

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