A mitochondria-targeted two-photon fluorogenic probe for the dual-imaging of viscosity and H<sub>2</sub>O<sub>2</sub> levels in Parkinson's disease models

Li, Hao; Xin, Chenqi; Zhang, Gaobin; Han, Xisi; Qin, Wenjing; Zhang, Chengwu; Yu, Changmin; Jing, Su; Li, Lin; Huang, Wei

doi:10.1039/c9tb00576e

Cited by 81 publications

(45 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Li et al. synthesized a mitochondria‐targeting two‐photon fluorescent probe 60 for simultaneous imaging of viscosity and hydrogen peroxide (H 2 O 2 ) [89] . Probe 60 consisted of naphthalene as the two‐photon fluorophore, pyridinium cation as the targetable moiety, phenylboronic acid pinacol ester as the H 2 O 2 responsive unit (Figure 28).…”

Section: Small Molecular Fluorescent Probes For Viscositymentioning

confidence: 99%

“…(B) TEM imaging and fluorescence imaging of wild‐type and parkin null Drosophila brains with probe 60 . Copyright 2019, Royal Society of Chemistry [89] . (C) The chemical structure of probe 61 and its response mechanism to viscosity and H 2 S. (D) Two‐photon fluorescence imaging of viscosity and H 2 S in Drosophila brain tissues.…”

Section: Small Molecular Fluorescent Probes For Viscositymentioning

confidence: 99%

See 1 more Smart Citation

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Xiao

Tang

2021

Chemistry A European J

117

115

View full text Add to dashboard Cite

Viscosity, as a vital microenvironment parameter, is tightly associated with multitudinous cellular processes and diseases. Recently, precise visualization of viscosity has started to arouse more and more interest. However, owing to the complicated character, it is still a huge challenge to directly observe viscosity in living systems. In this regard, mounting fluorescence probes are being increasingly fabricated to map viscosity inside live cells and small animals. In this minireview, the viscosity‐sensitive small molecular fluorescent probes used in bioimaging are comprehensively summarized, mainly focusing on the last three years. Moreover, the current challenges and opportunities for the development of viscosity‐specific fluorescent probes will be discussed.

show abstract

Section: Small Molecular Fluorescent Probes For Viscositymentioning

confidence: 99%

Section: Small Molecular Fluorescent Probes For Viscositymentioning

confidence: 99%

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Xiao

Tang

2021

Chemistry A European J

117

115

View full text Add to dashboard Cite

show abstract

“…[ 231 ] In 2019, Li et al designed the two‐photon fluorescent probe 146 (Mito‐LX, Figure 23) for the detection of mitochondrial H 2 O 2 level and viscosity ( Figure A). [ 246 ] Absorption of probe 146 was not susceptible to solutions of different viscosities, such as methanol and glycerin. No fluorescence was observed in methanol, whereas, it displayed a strong fluorescence signal with a high proportion of glycerol.…”

Section: Parkinson's Diseasementioning

confidence: 99%

Section: Parkinson's Diseasementioning

confidence: 99%

Fluorescent Diagnostic Probes in Neurodegenerative Diseases

et al. 2020

View full text Add to dashboard Cite

Both chronic and acute neurodegenerative diseases have been associated with high morbidity and mortality, along with the death of neurons in different areas of the brain, and there are few or no effective curative therapy options for treatment of patients. [3,4] These disorders are a major cause of concern for the health and quality of life in the aging society, since age is the greatest risk factor for neurodegenerative disease. [5] The World Health Organization has predicted that in next 20 years neurodegenerative disease will become the second most common cause of mortality after cardiovascular disease. [6] Neurodegenerative processes begin long before their clinical symptoms are evident, and evolve for years slowly and irreversibly. Hence, there is an urgent need to diagnose neurodegenerative disease as early as possible and to distinguish between different neurodegenerative disorders with shared and unique symptoms to facilitate decision making regarding choice of treatment. Timely diagnosis is important for the adoption of therapeutic modalities in clinical trials prior to moderate dementia exhibition, to appraise and apply antidotes to maximally preserve the cognitive functions or to slow down the course of these disorders. The ability to analyze and identify risk factors for cognitive deficit would represent an extraordinary advancement in the field of dementia. [5] The main modern diagnostic technologies for the early examination of neurodegenerative disease pathology, neuroimaging techniques, include radioligandsbased positron emission tomography (PET), 3D single-photon emission-computed tomography (SPECT), and structural magnetic resonance imaging (MRI). Although PET and SPECT are the most used imaging techniques for neurodegenerative diseases, their application is limited by high cost, involvement of hazardous radiolabeled compounds, need for sophisticated instruments, and application of complex data acquisition and analysis protocols. The MRI technique has relatively low spatial resolution as well as intrinsically poor sensitivity to distinguish morphological differences between disease biomarkers and the surrounding tissue. Recently, fluorescence diagnosis technology has become an attractive and potential alternative to diagnose and probe the progression of neurodegenerative diseases, because of being rapid, noninvasive, sensitive, simple, real-time, low-cost, and high-resolution in nature. [7] There are several uses for Neurodegenerative diseases are debilitating disorders that feature progressive and selective loss of function or structure of anatomically or physiologically associated neuronal systems. Both chronic and acute neurodegenerative diseases are associated with high morbidity and mortality along with the death of neurons in different areas of the brain; moreover, there are few or no effective curative therapy options for treating these disorders. There is an urgent need to diagnose neurodegenerative disease as early as possible, and to distinguish between different disorders with overlap...

show abstract

“…[ 28 ] Very recently, Liu and coworkers reported a single NIR fluorescent probe with a large Stokes shift for simultaneous imaging of mitochondrial viscosity and H 2 O 2 in Alzheimer's disease models. [ 31 ] To the best of our knowledge, only these three dual‐imaging fluorescent probes have been developed for monitoring both mitochondrial viscosity and H 2 O 2 , and the limited diseases models prevent us from delving into the intrinsic relationship between viscosity/H 2 O 2 and mitochondria‐ associated diseases due to the lack of suitable fluorescent probes and research on multiple diseases models, such as inflammation and malignant tumor models.…”

Section: Background and Originality Contentmentioning

confidence: 99%

A Mitochondria‐Specific Orange/Near‐Infrared‐Emissive Fluorescent Probe for Dual‐Imaging of Viscosity and H₂O₂ in Inflammation and Tumor Models

et al. 2021

View full text Add to dashboard Cite

Main observation and conclusion Elucidating the intrinsic relationship between viscosity/H2O2 and mitochondria‐associated diseases remains a great challenge owing to the lack of research on multiple diseases models, such as inflammation and malignant tumor models. In this work, we have developed a mitochondria‐specific orange/near‐infrared‐emissive fluorescent probe TTPB, for dual‐imaging of viscosity and H2O2 levels in two different channels. The probe exhibited a remarkable response to viscosity with NIR emission round 666 nm, and was highly sensitive to H2O2 in orange channel with emission peak at 586 nm. Moreover, TTPB has good mitochondria‐specific ability and permits individual detecting of viscosity in NIR channels and H2O2 levels in orange channel in living cells. More notably, TTPB was successfully applied to simultaneously image the viscosity and H2O2 levels in inflammation and cancer models.

show abstract

A mitochondria-targeted two-photon fluorogenic probe for the dual-imaging of viscosity and H₂O₂ levels in Parkinson's disease models

Abstract: Novel two-photon fluorogenic probe could simultaneously monitor changes in the mitochondrial viscosity and H2O2 levels using two different channels.

Cited by 81 publications

References 42 publications

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Fluorescent Diagnostic Probes in Neurodegenerative Diseases

A Mitochondria‐Specific Orange/Near‐Infrared‐Emissive Fluorescent Probe for Dual‐Imaging of Viscosity and H₂O₂ in Inflammation and Tumor Models

Contact Info

Product

Resources

About

A mitochondria-targeted two-photon fluorogenic probe for the dual-imaging of viscosity and H2O2 levels in Parkinson's disease models

Abstract: Novel two-photon fluorogenic probe could simultaneously monitor changes in the mitochondrial viscosity and H2O2 levels using two different channels.

Cited by 81 publications

References 42 publications

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Fluorescent Diagnostic Probes in Neurodegenerative Diseases

A Mitochondria‐Specific Orange/Near‐Infrared‐Emissive Fluorescent Probe for Dual‐Imaging of Viscosity and H2O2 in Inflammation and Tumor Models

Contact Info

Product

Resources

About

A mitochondria-targeted two-photon fluorogenic probe for the dual-imaging of viscosity and H₂O₂ levels in Parkinson's disease models

A Mitochondria‐Specific Orange/Near‐Infrared‐Emissive Fluorescent Probe for Dual‐Imaging of Viscosity and H₂O₂ in Inflammation and Tumor Models