A Mixture Containing Fermented <i>Achyranthes japonica</i> Nakai Ameliorates Osteoarthritis in Knee Joints of Monoiodoacetate-Injected Rats

Kim, Dakyung; Lee, Dasom; Oh, Deuk-Ja; Jeong, Hyun Cheol; Lee, Sung‐Jin; Sohn, Jungwoo; Kim, Ok Kyung; Lee, Jeongmin

doi:10.1089/jmf.2019.4552

Cited by 8 publications

(6 citation statements)

References 20 publications

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“…The interest in evaluating MSM effects on progenitor cells should be clear, since mesenchymal stem cells are the precursors of chondrocytes and osteoblasts, the cells which build the cartilage and bone, respectively, tissues which are mainly damaged by the aging process. Nevertheless, a few studies are available, which focused on evaluating the effects of MSM on osteogenic differentiation [24,25,33,34] or on preventing cartilage degradation [35][36][37][38]. These studies showed that MSM is capable of enhancing osteogenic differentiation as well as of preventing chondrocytes degradation.…”

Section: Discussionmentioning

confidence: 99%

Methylsulfonylmethane enhances MSC chondrogenic commitment and promotes pre-osteoblasts formation

et al. 2021

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Background Methylsulfonylmethane (MSM) is a nutraceutical compound which has been indicated to counteract osteoarthritis, a cartilage degenerative disorder. In addition, MSM has also been shown to increase osteoblast differentiation. So far, few studies have investigated MSM role in the differentiation of mesenchymal stem cells (MSCs), and no study has been performed to evaluate its overall effects on both osteogenic and chondrogenic differentiation. These two mutually regulated processes share the same progenitor cells. Methods Therefore, with the aim to evaluate the effects of MSM on chondrogenesis and osteogenesis, we analyzed the expression of SOX9, RUNX2, and SP7 transcription factors in vitro (mesenchymal stem cells and chondrocytes cell lines) and in vivo (zebrafish model). Real-time PCR as well Western blotting, immunofluorescence, and specific in vitro and in vivo staining have been performed. Student’s paired t test was used to compare the variation between the groups. Results Our data demonstrated that MSM modulates the expression of differentiation-related genes both in vitro and in vivo. The increased SOX9 expression suggests that MSM promotes chondrogenesis in treated samples. In addition, RUNX2 expression was not particularly affected by MSM while SP7 expression increased in all MSM samples/model analyzed. As SP7 is required for the final commitment of progenitors to preosteoblasts, our data suggest a role of MSM in promoting preosteoblast formation. In addition, we observed a reduced expression of the osteoclast-surface receptor RANK in larvae and in scales as well as a reduced pERK/ERK ratio in fin and scale of MSM treated zebrafish. Conclusions In conclusion, our study provides new insights into MSM mode of action and suggests that MSM is a useful tool to counteract skeletal degenerative diseases by targeting MSC commitment and differentiation.

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Section: Discussionmentioning

confidence: 99%

Methylsulfonylmethane enhances MSC chondrogenic commitment and promotes pre-osteoblasts formation

et al. 2021

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“…So far, researchers have focused only on the extract ingredients of AJN, which possess inhibitory effects against arthritis [ 53 , 54 ]. Moreover, the animal model they used was not a post-traumatic model such as monosodium iodoacetate-induced osteoarthritis animal model [ 55 ] or rabbit CIA model [ 56 ]. Furthermore, there is a lack of available information on the active compounds of AJN.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effects of IL-6-Mediated Matrix Metalloproteinase-3 and -13 by Achyranthes japonica Nakai Root in Osteoarthritis and Rheumatoid Arthritis Mice Models

et al. 2021

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Achyranthes japonica Nakai root (AJNR) is used to treat osteoarthritis (OA) and rheumatoid arthritis (RA) owing to its anti-inflammatory and antioxidant effects. This study investigated the inhibitory effects of AJNR on arthritis. AJNR was extracted using supercritical carbon dioxide (CO2), and its main compounds, pimaric and kaurenoic acid, were identified. ANJR’s inhibitory effects against arthritis were evaluated using primary cultures of articular chondrocytes and two in vivo arthritis models: destabilization of the medial meniscus (DMM) as an OA model, and collagenase-induced arthritis (CIA) as an RA model. AJNR did not affect pro-inflammatory cytokine (IL-1β, TNF-α, IL-6)-mediated cytotoxicity, but attenuated pro-inflammatory cytokine-mediated increases in catabolic factors, and recovered pro-inflammatory cytokine-mediated decreases in related anabolic factors related to in vitro. The effect of AJNR is particularly specific to IL-6-mediated catabolic or anabolic alteration. In a DMM model, AJNR decreased cartilage erosion, subchondral plate thickness, osteophyte size, and osteophyte maturity. In a CIA model, AJNR effectively inhibited cartilage degeneration and synovium inflammation in either the ankle or knee and reduced pannus formation in both the knee and ankle. Immunohistochemistry analysis revealed that AJNR mainly acted via the inhibitory effects of IL-6-mediated matrix metalloproteinase-3 and -13 in both arthritis models. Therefore, AJNR is a potential therapeutic agent for relieving arthritis symptoms.

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“…Like other botanical drug experiments, the studies have focused on changes in the expression of collagen, aggrecan, MMP-3, MMP-9, IL-1β, etc. and histological changes in the knee joints of rats and rabbits [ 56 - 58 ]. However, there has been no clinical trial on the effect of Achyranthes on OA.…”

Section: Functional Ingredients Of Health Functional Foods For Oa In ...mentioning

confidence: 99%

Medical treatment of osteoarthritis: botanical pharmacologic aspect

Park,

Lee

2024

J Rheum Dis

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Osteoarthritis (OA) is the most common form of arthritis, and its prevalence is expected to further increase as our society ages. Despite many approaches to cure OA, no drugs are currently proven to modulate the progression of OA. Nowadays, new OA treatment options are holistically developed and one of the approaches of treatment option is botanical drugs. Some botanical drugs for OA have shown both therapeutic effect comparable to refined drugs in small studies and fewer side effects. Hence, there are various health functional foods which are known to relieve symptoms of OA. However, since there are many botanical products, clinicians are not familiar to the efficacy of each botanical product, making it challenging to use them appropriately in clinical practice. Here, we summarize the botanical products available for treating OA, including prescription botanical drugs and health functional foods available in Korea. Further studies and the purification of effective molecules from botanical products will be necessary in future.

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A Mixture Containing Fermented Achyranthes japonica Nakai Ameliorates Osteoarthritis in Knee Joints of Monoiodoacetate-Injected Rats

Cited by 8 publications

References 20 publications

Methylsulfonylmethane enhances MSC chondrogenic commitment and promotes pre-osteoblasts formation

Methylsulfonylmethane enhances MSC chondrogenic commitment and promotes pre-osteoblasts formation

Inhibitory Effects of IL-6-Mediated Matrix Metalloproteinase-3 and -13 by Achyranthes japonica Nakai Root in Osteoarthritis and Rheumatoid Arthritis Mice Models

Medical treatment of osteoarthritis: botanical pharmacologic aspect

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