A model for cyst lumen expansion and size regulation via fluid secretion

Gin, Elan; Tanaka, Elly M.; Brusch, Lutz

doi:10.1016/j.jtbi.2010.03.021

Cited by 27 publications

(34 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This proportional dependence was also found through numerical simulation of the full time course in the original cyst model by (Gin et al, 2010). Gin et al, 2007Gin et al, , 2010 considered the solute pump strength I to be a function of luminal solute concentration and degree of cell stretching. The proportionality is expected to break down for larger (possibly non-physiological) parameter ranges as the non-linear solution (Eq.…”

Section: Models Of Fluid Transport and Tissue Morphogenesis Of Cystsmentioning

confidence: 63%

“…Analysis shows that the above model lets cysts expand and stretch on a short time scale and grow through cell proliferation on a long time scale (Gin et al, 2010). However, lumen growth is not unlimited in this model but approaches a stable steady state and hence shows lumen volume homeostasis.…”

Section: Models Of Fluid Transport and Tissue Morphogenesis Of Cystsmentioning

confidence: 92%

“…It is then an interesting question: which dependencies among tissue morphogenesis and fluid transport emerge at the tissue scale and would model cysts grow indefinitely? These questions have been addressed with the help of in silico models (Engelberg et al, 2011;Kim et al, 2009;Rejniak and Anderson, 2008;Grant et al, 2006) and mathematical analysis (Gin et al, 2010). Another question arises when signals are transmitted among the cells of the cyst by means of secreting ligands into the cyst lumen and sensing their concentration as observed for zebrafish mechanosensory organ formation (Durdu et al, 2014).…”

Section: Fluid Transport Across Epitheliamentioning

confidence: 99%

“…The model by Gin et al (2010) provides such an approach and is here summarized in a simplified form with constant solute secretion flux I per cell into the cyst lumen. Their model can be understood in terms of a core à la Kedem-Katchalsky of local osmosis and solute secretion as well as a shell given by the elastic pressure and its relationship with cell density dynamics.…”

Section: Models Of Fluid Transport and Tissue Morphogenesis Of Cystsmentioning

confidence: 99%

See 3 more Smart Citations

Mathematical modelling of fluid transport and its regulation at multiple scales

Chara

Brusch

2015

Biosystems

Self Cite

View full text Add to dashboard Cite

Section: Models Of Fluid Transport and Tissue Morphogenesis Of Cystsmentioning

confidence: 63%

Section: Models Of Fluid Transport and Tissue Morphogenesis Of Cystsmentioning

confidence: 92%

Section: Fluid Transport Across Epitheliamentioning

confidence: 99%

Section: Models Of Fluid Transport and Tissue Morphogenesis Of Cystsmentioning

confidence: 99%

See 2 more Smart Citations

Mathematical modelling of fluid transport and its regulation at multiple scales

Chara

Brusch

2015

Biosystems

Self Cite

View full text Add to dashboard Cite

“…The most recent computational models [1]-[4] relied on previously published quantitative data that described a few aspects of MDCK cyst growth in collagen cultures [5]. There is limited data available on the dynamics of cell number, cyst and lumen size, and mean cell size in Matrigel cultures.…”

Section: Introductionmentioning

confidence: 99%

MDCK Cystogenesis Driven by Cell Stabilization within Computational Analogues

et al. 2011

View full text Add to dashboard Cite

The study of epithelial morphogenesis is fundamental to increasing our understanding of organ function and disease. Great progress has been made through study of culture systems such as Madin-Darby canine kidney (MDCK) cells, but many aspects of even simple morphogenesis remain unclear. For example, are specific cell actions tightly coupled to the characteristics of the cell's environment or are they more often cell state dependent? How does the single lumen, single cell layer cyst consistently emerge from a variety of cell actions? To improve insight, we instantiated in silico analogues that used hypothesized cell behavior mechanisms to mimic MDCK cystogenesis. We tested them through in vitro experimentation and quantitative validation. We observed novel growth patterns, including a cell behavior shift that began around day five of growth. We created agent-oriented analogues that used the cellular Potts model along with an Iterative Refinement protocol. Following several refinements, we achieved a degree of validation for two separate mechanisms. Both survived falsification and achieved prespecified measures of similarity to cell culture properties. In silico components and mechanisms mapped to in vitro counterparts. In silico, the axis of cell division significantly affects lumen number without changing cell number or cyst size. Reducing the amount of in silico luminal cell death had limited effect on cystogenesis. Simulations provide an observable theory for cystogenesis based on hypothesized, cell-level operating principles.

show abstract

FIP 5 phosphorylation during mitosis regulates apical trafficking and lumenogenesis

et al. 2014

View full text Add to dashboard Cite

Apical lumen formation is a key step during epithelial morphogenesis. The establishment of the apical lumen is a complex process that involves coordinated changes in plasma membrane composition, endocytic transport, and cytoskeleton organization. These changes are accomplished, at least in part, by the targeting and fusion of Rab11/FIP5-containing apical endosomes with the apical membrane initiation site (AMIS). Although AMIS formation and polarized transport of Rab11/FIP5-containing endosomes are crucial for the formation of a single apical lumen, the spatiotemporal regulation of this process remains poorly understood. Here, we demonstrate that the formation of the midbody during cytokinesis is a symmetry-breaking event that establishes the location of the AMIS. The interaction of FIP5 with SNX18, which is required for the formation of apical endocytic carriers, is inhibited by GSK-3 phosphorylation at FIP5-T276. Importantly, we show that FIP5-T276 phosphorylation occurs specifically during metaphase and anaphase, to ensure the fidelity and timing of FIP5-endosome targeting to the AMIS during apical lumen formation.

show abstract

A model for cyst lumen expansion and size regulation via fluid secretion

Cited by 27 publications

References 43 publications

Mathematical modelling of fluid transport and its regulation at multiple scales

Mathematical modelling of fluid transport and its regulation at multiple scales

MDCK Cystogenesis Driven by Cell Stabilization within Computational Analogues

FIP 5 phosphorylation during mitosis regulates apical trafficking and lumenogenesis

Contact Info

Product

Resources

About