A model of experimental acute hematogenous pyelonephritis in the rat

Recent studies showed prominent antimicrobial activity of some plant extracts on some pathogenic microorganisms so we evaluated antimicrobial activity of aqueous extracts of clove and cinnamon using the agar well diffusion method. An in vivo study was carried out on 40 adult healthy male albino rats divided into four groups: Group 1: negative control group (received intragastric saline solution daily); Group 2: injected with mixed bacterial suspension of S. aureus and E.coli as a model of pyelonephritis then received intragastric saline solution daily; Group 3: injected with the same dose of mixed bacterial suspension then received intragastric clove extract 500 mg/kg/day; and Group (4): injected with mixed bacterial suspension then received intragastric cinnamon 500 mg/kg/day. Five rats from each group were sacrificed after 1 and 4 weeks. Serum and blood samples were collected for lysozymes activity and nitric oxide production, lymphocyte transformation test, as well as counting of both total and differential leukocytes and erythrocytes. Kidney samples were tested histopathologically. Both in vivo and in vitro results confirmed the efficacy of clove extract as natural antimicrobials and suggested the possibility of its use in treatment of such bacterial infections.

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“…Pyelonephritis was confirmed by differential leucocytic count and histopathological study of the kidneys. 12 …”

Section: Induction Of a Model Of Acute Hematogenous Pyelonephritis Inmentioning

confidence: 99%

Effect of clove and cinnamon extracts on experimental model of acute hematogenous pyelonephritis in albino rats: Immunopathological and antimicrobial study

Nassan

Mohamed

Abdel-Hafez

et al. 2015

Int J Immunopathol Pharmacol

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“…A less frequent pattern that resembles membranoproliferative glomerulonephritis type 1 by LM with glomerular deposits of IgM and C3 or IgG and C3 occurs in patients with ventriculo-vascular shunt infection by Staphylococcus epidermidis has also been described [1,2]. The classic glomerular pattern of injury associated with staphylococcal infection, including diffuse endocapillary proliferative and exudative glomerulonephritis on light microscopy (LM), glomerular immune deposits that stain for either IgG and C3 or C3 only on immunofluorescence (IF), and 'humpshaped' subepithelial deposits or at an early stage the presence of subendothelial or mesangial dense deposits on electron microscopy (EM) [1,2].…”

Section: Introductionmentioning

confidence: 99%

“…Classically, APIGN develops following streptococcal upper respiratory tract or skin infections [1,2]. More recently, other organisms, particularly Staphylococcus and Gram-negative bacteria, have been linked to APIGN in adults and elderly patients [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…The classic glomerular pattern of injury associated with staphylococcal infection, including diffuse endocapillary proliferative and exudative glomerulonephritis on light microscopy (LM), glomerular immune deposits that stain for either IgG and C3 or C3 only on immunofluorescence (IF), and 'humpshaped' subepithelial deposits or at an early stage the presence of subendothelial or mesangial dense deposits on electron microscopy (EM) [1,2].…”

Section: Introductionmentioning

confidence: 99%

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The Impact of Diabetes on Acute Post-Infectious Glomerulonephritis in Rats: An Experimental Study with Literature Review

Kfoury¹,

Alsuwaida²,

Alghonaim³

et al. 2017

J Clin Exp Nephrol

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Background: The concurrence of diabetes and nephritogenic infections may have a tremendous impact on the kidney. The approach to the two concurrent diseases is based on histological, immunofluorescence findings and most importantly on ultrastructural features. In this experiment our aim was to compare the pattern of injury seen in the kidney in animals with induced post-infectious glomerulonephritis with and without associated diabetes.

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“…Control animals received the same amount of saline solution. Pyelonephritis was confirmed by differential leucocytic count and histopathological study of the kidneys (15).…”

Section: Induction Ofa Model Ofacute Hematogenouspyelonephritis In Thmentioning

confidence: 99%

Immunopathological and Antimicrobial Effect of Black Pepper, Ginger and Thyme Extracts on Experimental Model of Acute Hematogenous Pyelonephritis in Albino Rats

Nassan

Mohamed

2014

Int J Immunopathol Pharmacol

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Recent studies showed prominent antimicrobial activity of various plant extracts on certain pathogenic microorganisms, therefore we prepared crude aqueous extracts of black pepper, ginger and thyme and carried out an in vitro study by measuring antimicrobial activity of these extracts using the agar well diffusion method. An in vivo study was carried out on 50 adult healthy male albino rats which were divided into 5 groups, 10 rats each. Group 1: negative control group which received saline solution intragastrically daily; Group 2: Positive control group, injected with mixed bacterial suspension of Ssaureus and Eicoli as a model of pyelonephritis, then received saline solution intragastrically daily; Group 3: injected with the same dose of mixed bacterial suspension, then received 100 mg/kg/day black pepper extract intragastrically; Group 4: injected with mixed bacterial suspension then received 500 mg/kg/day ginger extract intragastrically. Group 5: injected with mixed bacterial suspension then received 500 mg/kg/day thyme extract intragastrically. All groups were sacrificed after either 1 or 4 weeks. Serum and blood samples were collected for lysozyme activity estimation using agarose lysoplate, measurement of nitric oxide production, and lymphocyte transformation test as well as for counting both total and differential leukocytes and erythrocytes. Kidney samples were tested histopathologically. Both in vivo and in vitro results confirm the efficacy of these extracts as natural antimicrobials and suggest the possibility of using them in treatment procedures.

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A model of experimental acute hematogenous pyelonephritis in the rat

Cited by 9 publications

References 13 publications

Effect of clove and cinnamon extracts on experimental model of acute hematogenous pyelonephritis in albino rats: Immunopathological and antimicrobial study

Effect of clove and cinnamon extracts on experimental model of acute hematogenous pyelonephritis in albino rats: Immunopathological and antimicrobial study

The Impact of Diabetes on Acute Post-Infectious Glomerulonephritis in Rats: An Experimental Study with Literature Review

Immunopathological and Antimicrobial Effect of Black Pepper, Ginger and Thyme Extracts on Experimental Model of Acute Hematogenous Pyelonephritis in Albino Rats

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