A modified microstamping technique enhances polylysine transfer and neuronal cell patterning

Chang, J.C.S.; Brewer, Gregory J.; Wheeler, Bruce C.

doi:10.1016/s0142-9612(03)00116-9

Cited by 136 publications

(112 citation statements)

References 20 publications

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“…The stamps were sonicated 15 min in 70% ethanol, followed by 15 min in DI water, and 15 min in 10% SDS. Modification of the PDMS surface with SDS has been shown to enhance the transfer of poly-lysine to the substrate (Chang et al, 2003). The PDMS was quickly rinsed in DI water and blow dried with nitrogen.…”

Section: Stamping Proceduresmentioning

confidence: 99%

“…This can be achieved using an established stamping technique where a patterned PDMS stamp is used to transfer chemicals on to the substrate (Bani-Yaghoub et al, 2005;Charrier et al, 2006;Kumar et al, 1994;Offenhausser et al, 2007;Vogt et al, 2005;Wyart et al, 2002). In this study, we used poly-D-lysine (PDL), a commonly used cell attachment factor (Branch et al, 1998;Chang et al, 2003;Li and Folch, 2005). The bare surface of our chip, made of silicon nitride, is in contrast not conducive to neuronal adhesion.…”

Section: Introductionmentioning

confidence: 99%

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Cell placement and guidance on substrates for neurochip interfaces

Charrier

Martínez

Monette

et al. 2009

Biotech & Bioengineering

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Interface devices such as integrated planar patch‐clamp chips are being developed to study the electrophysiological activity of neuronal networks grown in vitro. The utility of such devices will be dependent upon the ability to align neurons with interface features on the chip by controlling neuronal placement and by guiding cell connectivity. In this paper, we present a strategy to accomplish this goal. Patterned chemical modification of SiN surfaces with poly‐d‐lysine transferred from PDMS stamps was used to promote adhesion and guidance of cryo‐preserved primary rat cortical neurons. We demonstrate that these neurons can be positioned and grown over microhole features which will ultimately serve as patch‐clamp interfaces on the chip. Biotechnol. Bioeng. 2010; 105: 368–373. © 2009 Wiley Periodicals, Inc.

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Section: Stamping Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cell placement and guidance on substrates for neurochip interfaces

Charrier

Martínez

Monette

et al. 2009

Biotech & Bioengineering

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“…[54][55][56] Furthermore, restricting neurons to patterns has been shown to enhance the cellular activity such as glutamine secretion and electrical activity as compared to random monocultures of neurons. [57,58] …”

Section: Patterned Versus Random Co-culturementioning

confidence: 99%

Primary Neuron/Astrocyte Co‐Culture on Polyelectrolyte Multilayer Films: A Template for Studying Astrocyte‐Mediated Oxidative Stress in Neurons

Kidambi

Lee

Chan

2008

Adv Funct Materials

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We engineered patterned co-cultures of primary neurons and astrocytes on polyelectrolyte multilayer (PEM) films without the aid of adhesive proteins/ligands to study the oxidative stress mediated by astrocytes on neuronal cells. A number of studies have explored engineering coculture of neurons and astrocytes predominantly using cell lines rather than primary cells owing to the difficulties involved in attaching primary cells onto synthetic surfaces. To our knowledge this is the first demonstration of patterned co-culture of primary neurons and astrocytes for studying neuronal metabolism. In our study, we used synthetic polymers, namely poly(diallyldimethylammoniumchloride) (PDAC) and sulfonated poly(styrene) (SPS) as the polycation and polyanion, respectively, to build the multilayers. Primary neurons attached and spread preferentially on SPS surfaces, while primary astrocytes attached to both SPS and PDAC surfaces. SPS patterns were formed on PEM surfaces, either by microcontact printing SPS onto PDAC surfaces or vice-versa, to obtain patterns of primary neurons and patterned co-cultures of primary neurons and astrocytes. We further used the patterned co-culture system to study the neuronal response to elevated levels of free fatty acids as compared to the response in separated monoculture by measuring the level of reactive oxygen species (ROS; a widely accepted marker of oxidative stress). The elevation in the ROS levels was observed to occur earlier in the patterned co-culture system than in the separated monoculture system. The results suggest that this technique may provide a useful tool for engineering neuronal co-culture systems, that may more accurately capture neuronal function and metabolism, and thus could be used to obtain valuable

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“…The CPEDOT NTs were attached to the APS-treated IMA surface via covalent linkages between amino groups (-NH 2 ) on the substrate surface and the carboxylic groups (-COOH) of the CPEDOT NTs. Consecutively, uniform-particle-shaped nanovesicles were immobilized on the stable CPEDOT NT substrate using poly-D-lysine (PDL), a synthetic amino acid chain widely used as a coating to enhance cell attachment by its positive charge 36,53 . The nanovesicles derived from cells have same property to cell membranes and the treatment of PDL allowed efficient immobilization process.…”

Section: Resultsmentioning

confidence: 99%

Human dopamine receptor nanovesicles for gate-potential modulators in high-performance field-effect transistor biosensors

Kwon¹

2016

Intrinsic Act

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The development of molecular detection that allows rapid responses with high sensitivity and selectivity remains challenging. Herein, we demonstrate the strategy of novel bio-nanotechnology to successfully fabricate high-performance dopamine (DA) biosensor using DA Receptor-containing uniform-particle-shaped Nanovesicles-immobilized Carboxylated poly(3,4-ethylenedioxythiophene) (CPEDOT) NTs (DRNCNs). DA molecules are commonly associated with serious diseases, such as Parkinson's and Alzheimer's diseases. For the first time, nanovesicles containing a human DA receptor D1 (hDRD1) were successfully constructed from HEK-293 cells, stably expressing hDRD1. The nanovesicles containing hDRD1 as gate-potential modulator on the conducting polymer (CP) nanomaterial transistors provided high-performance responses to DA molecule owing to their uniform, monodispersive morphologies and outstanding discrimination ability. Specifically, the DRNCNs were integrated into a liquid-ion gated field-effect transistor (FET) system via immobilization and attachment processes, leading to high sensitivity and excellent selectivity toward DA in liquid state. Unprecedentedly, the minimum detectable level (MDL) from the field-induced DA responses was as low as 10 pM in real-time, which is 10 times more sensitive than that of previously reported CP based-DA biosensors. Moreover, the FET-type DRNCN biosensor had a rapid response time (,1 s) and showed excellent selectivity in human serum.

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A modified microstamping technique enhances polylysine transfer and neuronal cell patterning

Cited by 136 publications

References 20 publications

Cell placement and guidance on substrates for neurochip interfaces

Cell placement and guidance on substrates for neurochip interfaces

Primary Neuron/Astrocyte Co‐Culture on Polyelectrolyte Multilayer Films: A Template for Studying Astrocyte‐Mediated Oxidative Stress in Neurons

Human dopamine receptor nanovesicles for gate-potential modulators in high-performance field-effect transistor biosensors

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