A molecular genetic analysis of insertions in the bronze locus in maize

Dooner, Hugo K.; Weck, E.; Adams, Sharon; Ralston, Edward J.; Favreau, Mitchell; English, James J.

doi:10.1007/bf00425430

Cited by 93 publications

(49 citation statements)

References 25 publications

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“…Fully colored kernels were also recovered on testcross progeny recovered from all three tester lines, indicative of germinal excision events. Ds insertions in bz1 and a2 are in exon sequences; thus, few excisions events are expected to restore gene function (Dooner et al 1985;E. Vollbrecht, personal communication).…”

Section: Resultsmentioning

confidence: 99%

Ac-Immobilized, a Stable Source of Activator Transposase That Mediates Sporophytic and Gametophytic Excision of Dissociation Elements in Maize

Conrad

Brutnell

2005

Genetics

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We have identified and characterized a novel Activator (Ac) element that is incapable of excision yet contributes to the canonical negative dosage effect of Ac. Cloning and sequence analysis of this immobilized Ac (Ac-im) revealed that it is identical to Ac with the exception of a 10-bp deletion of sequences at the left end of the element. In screens of 6800 seeds, no germinal transpositions of Ac-im were detected. Importantly, Ac-im catalyzes germinal excisions of a Ds element resident at the r1 locus resulting in the recovery of independent transposed Ds insertions in 4.5% of progeny kernels. Many of these transposition events occur during gametophytic development. Furthermore, we demonstrate that Ac-im transactivates multiple Ds insertions in somatic tissues including those in reporter alleles at bronze1, anthocyaninless1, and anthocyaninless2. We propose a model for the generation of Ac-im as an aberrant transposition event that failed to generate an 8-bp target site duplication and resulted in the deletion of Ac end sequences. We also discuss the utility of Ac-im in two-component Ac/Ds gene-tagging programs in maize.

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Section: Resultsmentioning

confidence: 99%

Ac-Immobilized, a Stable Source of Activator Transposase That Mediates Sporophytic and Gametophytic Excision of Dissociation Elements in Maize

Conrad

Brutnell

2005

Genetics

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“…Severa1 genes encoding enzymatic activities have been identified and cloned via transposon tagging. These include the structural genes for dihydroquercetin reductase (A 7) (O'Reilly et al, 1985), chalcone synthase (C2) , UDPgIucose-flavonol glucosyltransferase (bronze, Bz7 ) (Fedoroff, Furtek, and Nelson, 1984;Dooner et al, 1985), and Bronze-2 (McLaughlin and Walbot, 1987;Theres, Scheele, and Starlinger, 1987). Although no gene product has as yet been identified for Bronze-2 ( B z~) , precursor feeding studies and accumulated intermediates indicate that it acts late in the pathway (Reddy and Coe, 1962;McCormick, 1978).…”

Section: Introductionmentioning

confidence: 99%

Genetic regulation and photocontrol of anthocyanin accumulation in maize seedlings.

Taylor

Briggs

1990

Plant Cell

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The flavonoid pathway leading to anthocyanin biosynthesis in maize is controlled by multiple regulatory genes and induced by various developmental and environmental factors. We have investigated the effect of the regulatory loci R, B, and f / on anthocyanin accumulation and on the expression of four genes ( C2, A l , 621, and 822) in the biosynthetic pathway during an inductive light treatment. The results show that light-mediated anthocyanin biosynthesis is regulated solely by R; the contributions of B and f / are negligible in young seedlings. lnduction of the A1 and Bz2 genes by high fluence-rate white light requires the expression of a dominant R allele, whereas accumulation of C2 and Bzl mRNA occurs with either a dominant or recessive allele at R. A1 and 822 mRNA accumulate only in response to high fluence-rate white light, but Bzl is fully expressed in dim red light. Some C2 mRNA is induced by dim red light, but accumulation is far greater in high fluence-rate white light. Furthe'more, expression from both dominant and recessive alleles of the regulatory gene R is enhanced by high fluence-rate white light. Seedlings with a recessive allele at R produce functional chalcone synthase protein (the C2 gene product) but accumulate no anthocyanins, suggesting that, in contrast to the R-mediated coordinate regulation of C2 and Bzl observed in the aleurone, C2 expression in seedlings is independent of R and appears to be regulated by a different light-sensitive pathway.

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“…The Ds-r element contains both the GUS gene driven by a 0.8 kb fragment of the CaMV 35S promoter and parts of plasmid pA-CYC 184 (origin of replication and chloramphenicol resistance gene) between the 586 bp 5' Ac terminal sequence and the 448 bp 3' Ac terminal sequence [46]. The construction of the modified Ac element present between the borders of the T-DNA of SLJ1515 was initiated from a clone of the maize bronze allele bz-m2 (Ac) [17]. A Cla I site was inserted by oligonucleotide mutagenesis, 82 bp 3' to the mapped polyadenylation site [34].…”

Section: Plant Materialsmentioning

confidence: 99%

“…The involvement of transposable elements in the generation of internal deletions has been described for Ac [17,26,47] and for several other transposable elements of the so-called "Ac family' [10] such as the Drosophila P [41] and hobo [8] elements. Here we show that sequences not homologous to Ac but inserted between Ac terminal sequences can also be involved in deletions.…”

Section: The Generation Of Ds-r Deletion Derivativesmentioning

confidence: 99%

Transposition pattern of a modified Ds element in tomato

et al. 1993

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Several aspects of transposition of an in vitro modified Ds element are described. This Ds element, designated Ds-r, is equipped with bacterial plasmid sequences and can, therefore, be rescued from the plant genome. Our results indicate that the Ds-r element has a 'late' timing of transposition from T-DNAs. This feature of the element might be advantageous for tagging experiments because it leads to independently transposed germinally transmitted elements. Furthermore, it is shown that Ds-r transposition generates clusters of insertions, indicating that 'genes to be tagged' should be located in genomic regions covered by insertions.

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A molecular genetic analysis of insertions in the bronze locus in maize

Cited by 93 publications

References 25 publications

Ac-Immobilized, a Stable Source of Activator Transposase That Mediates Sporophytic and Gametophytic Excision of Dissociation Elements in Maize

Ac-Immobilized, a Stable Source of Activator Transposase That Mediates Sporophytic and Gametophytic Excision of Dissociation Elements in Maize

Genetic regulation and photocontrol of anthocyanin accumulation in maize seedlings.

Transposition pattern of a modified Ds element in tomato

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